Kappa Opioid Receptors on Three Related Thymoma Cell Lines

Differences in Receptor-Effector Coupling
  • Jean M. Bidlack
  • David B. Joseph
  • Diane M. P. Lawrence
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 373)


The search for opioid binding sites on leukocytes has been difficult. The presence of opioid binding sites has been reported on human and murine leukocytes (1–3) as well as on some cell lines (4,5). However, in contrast to brain opioid receptors, these sites do not exhibit all of the classical opioid binding characteristics, such as stereoselectivity and high affinity for both alkaloids and peptides (6). In addition, agonist-induced functional responses have not been correlated with these binding sites. It seems likely that only a small population of leukocytes actually express opioid receptors, and possibly only under certain conditions. If this is the case, binding studies with mixed cell populations may not show a detectable signal-to-noise ratio, even when receptors are present.


Opioid Receptor Adenylyl Cyclase GTPase Activity Adenylyl Cyclase Activity Kappa Opioid Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. J. Madden, R. M. Donahoe, J. Zwemer-Collins, D. A. Shafer and A. Falek, Binding of naloxone to human T lymphocytes, Biochem. Pharmacol. 36: 4103–4109 (1987).PubMedCrossRefGoogle Scholar
  2. 2.
    H. Ovadia, P. Nitsan and O. Abramsky, Characterization of opiate binding sites on membranes of rat lymphocytes,.J. Neuroimmunol. 21:93–102 (1989).PubMedCrossRefGoogle Scholar
  3. 3.
    S. Roy, B.-L. Ge, S. Ramakrishnan, N. M. Lee and H. H. Loh, [3H]Morphine binding is enhanced by IL-1-stimulated thymocyte proliferation, FEBS Lett. 287: 93–96 (1991).PubMedCrossRefGoogle Scholar
  4. 4.
    E. Fiorica and S. Spector, Opioid binding site in EL-4 thymoma cell line. Life Sci. 42:199–206 (1988).PubMedCrossRefGoogle Scholar
  5. 5.
    D. J. J. Carr, B. R. DeCosta, C.-H. Kim, A. E. Jacobson, V. Guarcello, K. C. Rice and J. E. Blalock, Opioid receptors on cells of the immune system: evidence for δ- and K-classes, J. Endocrinol. 122:161–168 (1989).PubMedCrossRefGoogle Scholar
  6. 6.
    N. E. S. Sibinga and A. Goldstein, Opioid peptides and opioid receptors in cells of the immune system, Ann. Rev. Immunol. 6:219–249 (1988).CrossRefGoogle Scholar
  7. 7.
    J. M. Bidlack, L. D. Saripalli and D. M. P. Lawrence, κ-Opioid binding sites on a murine lymphoma cell line, Eur. J. Pharmacol. 227:257–265 (1992).PubMedCrossRefGoogle Scholar
  8. 8.
    D. M. P. Lawrence and J. M. Bidlack, The kappa opioid receptor expressed on the mouse R1.1 thymoma cell line is coupled to adenylyl cyclase through a pertussis toxin-sensitive guanine nucleotide-binding regulatory protein, J. Pharmacol. Exp. Ther 266:1678–1683 (1993).PubMedGoogle Scholar
  9. 9.
    P. Ralph, Retention of lymphocyte characteristics by myelomas and θ+-lymphomas: sensitivity to cortisol and phytohemagglutinin, J. Immunol. 110:1470–1475 (1973).PubMedGoogle Scholar
  10. 10.
    R. S. Zukin, M. Eghbali, D. Olive, E. M. Unterwald and A. Tempel, Characterization and visualization of rat and guinea pig brain K opioid receptors: evidence for κ1 and κ2 opioid receptors, Proc. Natl. Acad. Sci. U.S.A. 85:4061–4065 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    J. A. Clark, L. Liu, M. Price, B. Hersh, M. Edelson and G. W. Pasternak, Kappa opiate receptor multiplicity: evidence for two U50,488-sensitive κ1 subtypes and a novel κ3 subtype, J. Pharmacol. Exp. Ther 251:461–468 (1989).PubMedGoogle Scholar
  12. 12.
    G. P. Brown, K. M. Standifer and G. W. Pasternak, Biochemical characterization of κ1 opiate binding sites in R1.1 thymoma cells, Soc. Neurosci. Abstr 19:1154 (1993).Google Scholar
  13. 13.
    D. M. P. Lawrence, D. B. Joseph and J. M. Bidlack, Kappa opioid receptors expressed on three related thymoma cell lines: differences in receptor-effector coupling, Biochem. Pharmacol. 49:81–89 (1995).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Jean M. Bidlack
    • 1
  • David B. Joseph
    • 1
  • Diane M. P. Lawrence
    • 1
  1. 1.Department of PharmacologyUniversity of Rochester School of Medicine and DentistryRochesterUSA

Personalised recommendations