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Lymphokine mRNA Expression in the Human Intestinal Mucosa and PBL Determined by Quantitative RT/PCR

  • L. Braun-Elwert
  • G. E. Mullin
  • S. P. James
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 371)

Abstract

Mucosal T-lymphocytes are unique in that they express predominantly phenotypic markers of activation such as IL-2R1 and HML-12 and the “memory” cell phenotype CD45RO3-5 but lack the lymph node homing receptor Leu-8.6 After challenge with specific Ag in vitro they do not proliferate but provide help for B-lymphocytes.7 Furthermore, after in vitro activation they express IL-2, IFNγ, IL-4 and IL-58, lymphokines which are thought to be critical for host defense in the normal intestinal mucosa. Since relatively little is known about lymphokine production in the normal human intestine, we wanted to determine the in situ expression of these lymphokines. So far, most of the information has been obtained from in vitro studies of intestinal lymphocytes that were isolated from surgical specimens using enzymatic methods. In this study, colonoscopic biopsies from normal individuals were used as a source of intestinal lymphocytes. Since only a small amount of total cellular RNA was available for study, we developed a reverse transcription (RT)-PCR method to determine lymphokine mRNA that was both very sensitive and quantitative.

Keywords

Template Molecule Lymphokine Production Colonoscopic Biopsy Normal Intestinal Mucosa Human Intestinal Mucosa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • L. Braun-Elwert
    • 1
  • G. E. Mullin
    • 2
    • 3
  • S. P. James
    • 4
  1. 1.Abt. f. Innere Medizin mit Schwer-punkt Gastroenterologie, Leiter Prof. Dr. E.O. RieckenMed. Klinik der FU BerlinBerlin 45Germany
  2. 2.Mucosal Immunity Section, Laboratory of Clinical InvestigationNational Institute of Allergy and Infectious DiseasesBethesdaUSA
  3. 3.Division of Gastroenterology, Department of MedicineThe Johns Hopkins HospitalBaltimoreUSA
  4. 4.Division of GastroenterologyUniversity of Maryland at BaltimoreBaltimoreUSA

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