The Leucocyte Protein L1 (Calprotectin): A Putative Nonspecific Defence Factor at Epithelial Surfaces

  • Per Brandtzaeg
  • Tor-Øivind Gabrielsen
  • Inge Dale
  • Fredrik Müller
  • Martin Steinbakk
  • Magne K. Fagerhol
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 371)

Abstract

The leucocyte antigen L1 is a calcium-binding, highly immunogenic molecule which predominantly consists of different polypeptide chains adding up to an Mr≈36 K noncovalently stabilized complex.1 Peptides I and II are virtually identical (L1H chain) whereas the smaller peptide III is structurally and antigenically in the main different (L1L chain).2 L1 was discovered more than 10 years ago as a major cytosol protein fraction (50–60%) of neutrophilic granulocytes.3 In addition to being expressed by most circulating (and emigrated) neutrophils and monocytes4, it is also found in a subset of reactive tissue macrophages and many tissue eosinophils.5 Its abundant occurrence in vivo as an Mr≈36 K peptide complex both in neutrophils and monocytes has recently been confirmed6,7.

Keywords

Zinc Migration Agar Polypeptide Cytosol 

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References

  1. 1.
    I. Dale, M. K. Fagerhol, and I. Naesgaard, Eur. J. Biochem. 134: 1 (1983).PubMedCrossRefGoogle Scholar
  2. 2.
    H. B. Berntzen and M. K. Fagerhol, Scand. J. Clin. Lab. Invest. 50: 769 (1990).PubMedCrossRefGoogle Scholar
  3. 3.
    M. K. Fagerhol, K. B. Andersson, C.-F. Naess-Andresen, P. Brandtzaeg, and I. Dale, in: “Stimulus Response Coupling: The Role of Intracellular Calcium-Binding Proteins”, N. L. Smith, and T. R. Dedman, eds, pp. 187, CRC Press, Boca Raton, Florida (1990).Google Scholar
  4. 4.
    I. Dale, P. Brandtzaeg, M. K. Fagerhol, and H. Scott, Am. J. Clin. Path. 84: 24 (1985).PubMedGoogle Scholar
  5. 5.
    P. Brandtzaeg, I. Dale, and M. K. Fagerhol, Am. J. Clin. Path. 87: 681 (1987).PubMedGoogle Scholar
  6. 6.
    S. Teigelkamp, R. S. Bhardwaj, J. Roth, G. Meinardus-Hager, M. Karast, and C. Sorg, J. Biol. Chem. 266: 13462 (1991).PubMedGoogle Scholar
  7. 7.
    J. Edgeworth, M. Gorman, R. Bennett, P. Freemont, and N. Hogg, J. Biol. Chem. 266: 7706 (1991).PubMedGoogle Scholar
  8. 8.
    K. Odink, N. Cerletti, J. Brüggen, R. G. Clerc, L. Tarcsay, G. Zwadlo, G. Gerhards, R. Schlegel, and C. Sorg, Nature 330: 80 (1987).PubMedCrossRefGoogle Scholar
  9. 9.
    J. R. Dorin, M. Novak, R. E. Hill, D. J. H. Brock, D. S. Secher, and V. van Heyningen, Nature 326: 614 (1987).PubMedCrossRefGoogle Scholar
  10. 10.
    K. B. Andersson, K. Sletten, H. B. Berntzen, I. Dale, P. Brandtzaeg, E. Jellum, and M. K. Fagerhol, Scand. J. Immunol. 28: 241 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    M. Steinbakk, C.-F. Naess-Andresen, E. Lingaas, I. Dale, P. Brandtzaeg, and M. K. Fagerhol, Lancet 336: 763 - 765 (1990).PubMedCrossRefGoogle Scholar
  12. 12.
    P. G. Sohnle, C. Collins-Lech, and J. H. Weissner, J. Infect. Dis. 163: 187 - 192 (1991).PubMedCrossRefGoogle Scholar
  13. 13.
    Editorial, Lancet 338: 855 (1991).CrossRefGoogle Scholar
  14. 14.
    P. Brandtzaeg, I. Dale, and M. K. Fagerhol, Am. J. Clin. Path. 87: 700 (1987).PubMedGoogle Scholar
  15. 15.
    T.-Ø. Gabrielsen, I. Dale, P. Brandtzaeg, P. S. Hoel, M. K. Fagerhol, T. Eeg Larsen, and P. O. Thune, J. Am. Acad. Dermatol. 15: 173 (1986).PubMedCrossRefGoogle Scholar
  16. 16.
    P. Brandtzaeg, D. B. Jones, D. J. Flavell, and M. K. Fagerhol, J. Clin. Pathol. 41: 963 (1988).PubMedCrossRefGoogle Scholar
  17. 17.
    S. E. Kelly, D. B. Jones, and S. Fleming. J. Pathol. 159: 17 (1989).PubMedCrossRefGoogle Scholar
  18. 18.
    M. M. Wilkinson, A. Busuttil, C. Hay ward, D. J. H. Brock, J. R. Dorin, and V. van Heyningen, J. Cell Sci. 91: 221 (1988).PubMedGoogle Scholar
  19. 19.
    C. Barthe, C. Figarella, J. Carrere, and O. Guy-Crotte, Biochim. Biophys. Acta 1096: 175 (1991).PubMedCrossRefGoogle Scholar
  20. 20.
    G. Zwadlo, J. Brüggen, G. Gerhards, R. Schlegel, and C. Sorg, Clin. Exp. Immunol. 72: 510 (1988).Google Scholar
  21. 21.
    J. Delabie, C. De Wolf-Peeters, J. J. van den Oord, and V. J. Desmet, Clin. Exp. Immunol. 81: 123 (1990).PubMedCrossRefGoogle Scholar
  22. 22.
    D. Kligman and D. C. Hilt, TIBS 13: 437 (1988).PubMedGoogle Scholar
  23. 23.
    S. E. Kelly, J. A. A. Hunter, D. B. Jones, B. R. Clark, and S. Fleming, Br. J. Dermatol. 124: 403 (1991).Google Scholar
  24. 24.
    P. Freemont, N. Hogg, and J. Edgeworth, Nature 339: 516 (1989).PubMedCrossRefGoogle Scholar
  25. 25.
    J. Edgeworth, P. Freemont, and N. Hogg, Nature 342: 189 (1989).PubMedCrossRefGoogle Scholar
  26. 26.
    P. G. Sohnle, C. Collins-Lech, and J. H. Wiessner, J. Infect. Dis. 164: 137 (1991).PubMedCrossRefGoogle Scholar
  27. 27.
    F. Müller, S. S. Frøland, P. Brandtzaeg, and M. Fagerhol, Clin. Infect. Dis., 16: 301 (1993).PubMedCrossRefGoogle Scholar
  28. 28.
    F. Müller, S. S. Frøland, M. Hvatum, J. Radl, and P. Brandtzaeg, Clin. Exp. Immunol. 83: 203 (1991).PubMedCrossRefGoogle Scholar
  29. 29.
    J. J. Pollock, R. P. Santarpia, H. M. Heller, L. Xu, K. Lai, J. Fuhrer, H. W. Kaufman, and R. T. Steigbigel, J. Acquir. Immune Defic. Syndr. 5: 610 (1992).PubMedGoogle Scholar
  30. 30.
    S. Murao, F. R. Collart, and E. Huberman, Cell Growth Differentiation 1: 447 (1990).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Per Brandtzaeg
    • 1
  • Tor-Øivind Gabrielsen
    • 1
    • 2
  • Inge Dale
    • 1
  • Fredrik Müller
    • 1
    • 3
  • Martin Steinbakk
    • 4
  • Magne K. Fagerhol
    • 4
  1. 1.Laboratory for Immunohistochemistry and Immunopathology (LIIPAT)Institute of PathologyNorway
  2. 2.Department of DermatologyNorway
  3. 3.Institute of BacteriologyThe National HospitalRikshospitaletNorway
  4. 4.Departments of Microbiology and ImmunologyUllevål Hospital, University of OsloOsloNorway

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