Analysis of Intestinal Intraepithelial Lymphocyte (IEL) T Cells in Mice Expressing Anti-CD8 Immunoglobulin Transgenes

  • Wilhelm K. Aicher
  • Hermann Eibel
  • Kohtaro Fujihashi
  • Tilman Boehm
  • Kenneth W. Beagley
  • Jerry R. McGhee
  • Hiroshi Kiyono
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 371)

Abstract

In mouse, intraepithelial lymphocytes (IEL) of the small intestine contain a large number of CD4-, CD8+ T cells. Smaller numbers of IEL are CD4+, CD8-, CD4-, CD8- (double negative, DN) or CD4+, CD8+ (double positive, DP). An unusual feature of IEL is their predominant expression of γ/δ TCR (up to 75%) and rather small numbers of α/β TCR+ populations.1,2 Several lines of evidence suggested that IEL develop in a thymus independent pathway (for review see3). The γ/δ TCR+ IEL express an α/α homodimer CD8 molecule, which does not react with Ly-3, an antibody that stains virtually all other peripheral CD8+ T cells, including the CD4+, CD8+ thymic T cells.4 Experiments with thymectomized and irradiated mice which were reconstituted with bone marrow or fetal liver resulted in regeneration of IEL T cells and suggested their thymus independent development.5,6

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    T. Goodman and L. Lefrancois, Nature 333: 855 (1988).PubMedCrossRefGoogle Scholar
  2. 2.
    K. Ito, M. Bonneville, Y. Takagaki, N. Nakanishi, O. Kanagawa, E. G. Krecko, and S. Tonegawa, Proc. Natl. Acad. Sci. USA 68: 631 (1989).CrossRefGoogle Scholar
  3. 3.
    L. Lefrancois, Immunol. Today 12: 436 (1991).Google Scholar
  4. 4.
    D. Guy-Grand, N. Cerf-Bensussan, B. Malissen, M. Malissen-Seris, C. Briottet, and P. Vassalli, J. Exp. Med. 173: 471 (1991).PubMedCrossRefGoogle Scholar
  5. 5.
    A. Fergusson and D. V. M. Parrott, Clin. Exp. Immunol 12: 477 (1972).Google Scholar
  6. 6.
    R. L. Mosley, D. Styre, and J. Klein, J. Immunol 145: 1369 (1990).PubMedGoogle Scholar
  7. 7.
    M. A. Oettinger, D. G. Schatz, C. Gorka, and D. Baltimore, Science 248: 1517 (1990).PubMedCrossRefGoogle Scholar
  8. 8.
    R. Mombaerts, J. Iacomini, R. S. Johnson, K. Herrup, S. Tonegawa, and V. E. R. Papaioannou, Cell 68: 869 (1992).Google Scholar
  9. 9.
    Y. Shinkai, G. Rathbun, V. Steward, M. Mendelsohn, J. Charron, M. Datta, F.Young, A.M. Stall, and F. W. Alt, Cell 68: 855 (1992).PubMedCrossRefGoogle Scholar
  10. 10.
    D. Guy-Grand, C. Vanden Broecke, C. Briottet, M. Malassis-Seris, F. Selz, and P.Vassalli, Eur. J. Immunol 22: 505 (1992).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Wilhelm K. Aicher
    • 1
    • 2
  • Hermann Eibel
    • 2
  • Kohtaro Fujihashi
    • 1
  • Tilman Boehm
    • 2
  • Kenneth W. Beagley
    • 1
  • Jerry R. McGhee
    • 1
  • Hiroshi Kiyono
    • 1
  1. 1.The University of Alabama at BirminghamBirminghamUSA
  2. 2.The University of Freiburg and MPI for Immunobiology FreiburgGermany

Personalised recommendations