Subcellular Control of Oxygen Transport

  • E. Takahashi
  • K. Doi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 393)


According to the Fick’s law of diffusion, oxygen pressure of mitochondrial inner membrane (PMt) is defined by the following equation;
$${P_{Mt}} = {P_{cap}} - {\dot V_{{O_2}}} \cdot R,$$
where Pcap, \({\dot V_{{O_2}}}\),and R denote Po2 of capillary blood, flux of oxygen into mitochondria (oxygen consumption rate of the cell), and diffusion resistance of tissue, respectively. R is a lumped parameter and includes diffusion resistance of plasma, capillary wall, extracellular fluid, plasma membrane, cytosol, and mitochondrial inner membrane. Therefore, the oxygen pressure gradient between capillary blood and mitochondria is represented by \({\dot V_{{O_2}}} \cdot R\). Magnitude of the oxygen pressure gradient in vivo appears so large (> 20–25 Torr) that intracellular (cytosolic) Po2 of normal beating heart may be around P50 of myoglobin, i.e., ~3 Torr (11). Furthermore, additional oxygen pressure gradients between cytosol and mitochondrial inner membrane result in quite low Po2at mitochondrial enzymes.


Oxygen Transport Oxygen Consumption Rate Oxygen Flux Simulated Ischaemia HEPES Buffer Solution 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • E. Takahashi
    • 1
  • K. Doi
    • 1
  1. 1.Department of PhysiologyYamagata University School of MedicineYamagataJapan

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