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Coronaviruses in Polarized Epithelial Cells

  • J. W. A. Rossen
  • C. P. J. Bekker
  • W. F. Voorhout
  • M. C. Horzinek
  • A. Van Der Ende
  • G. J. A. M. Strous
  • P. J. M. Rottier
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively.

Keywords

Basolateral Membrane Apical Side Basolateral Side Mouse Hepatitis Virus Permeable Support 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Tucker, S. P., and R. W. Compans. Virus infection of polarized epithelial cells. Adv. Virus Res. 1993;42:187–247.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • J. W. A. Rossen
    • 1
  • C. P. J. Bekker
    • 1
  • W. F. Voorhout
    • 2
  • M. C. Horzinek
    • 1
  • A. Van Der Ende
    • 3
  • G. J. A. M. Strous
    • 3
  • P. J. M. Rottier
    • 1
  1. 1.Institute of VirologyUtrecht UniversityUtrechtThe Netherlands
  2. 2.Department of Functional MorphologyUtrecht UniversityUtrechtThe Netherlands
  3. 3.Laboratory of Cell BiologyUtrecht UniversityUtrechtThe Netherlands

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