Abstract
Gene therapy is emerging as a new and exciting therapeutic modality for cardiovascular pathology. The work reported here was carried out in the National Heart, Lung and Blood Institute (NHLBI) in Bethesda, MD, USA, where genetically engineered endothelial cells were used to seed endovascular prostheses and cell adhesion to the prostheses was tested both in vitro and in vivo. Two catheter based systems were used to deliver genes to the arterial wall cells in vivo, employing retroviral and adenoviral vectors. Efficient gene transfer to vascular cells in vivo was achieved with adenoviral vectors.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Mulligan RC. The basic science of gene therapy. Science. 1993;260:926–932.
Anderson WF. Human gene therapy. Science. 1992;256:808–813.
Swain JL. Gene therapy; anew approach to the treatment of cardiovascular disease. Circulation. 1989;80:1495–1496.
Schneider M, French BA. The advent of Adenovirus-Gene therapy for cardiovascular disease. Circulation. 1993;88:1937–1942.
Nabel EG, Plautz G, Boyce FM, Stanley JC, Nabel GJ. Recombinant gene expression in vivo within endothelial cells of the arterial wall. Science. 1989;244:1342–1344.
Flugelman MY, Leon MB, Bowman RL, Virmani R, Anderson WF, Dichek DA. Retention of seeded cells on balloon expanded stents under flow conditions. Circulation. 1990;82(Supl. III):72.
Flugelman MY, Virmani R, Leon MB, Bowman RL, Dichek DA. Genetically engineered endothelial cells remain adherent and viable after stent deployment and exposure to flow in vitro. Circ Res. 1992;70:348–354.
Flugelman MY, Rome JJ, Virmani R, Newman KD, Dichek DA. Detection of genetically engineered endothelial cells seeded on endovascular prosthesis ten days after in vivo deployment. J Mol Cell Cardiol. 1993;25 (supp I):S.38.
Rome JJ, Shayani V, Flugelman MY, Newman KD, Farb A, Virmani R, Dichek DA. Anatomic barriers determine the distribution of in vivo gene transfer into the arterial wall: modeling with microscopic tracer particles and verification with a recombinant adenoviral vector. Atherosclerosis & Thrombosis 1994;14:148–161.
Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature. 1993;362:801–809.
Flugelman MY, Virmani R, Correa R, Yu Z-X, Farb A, Leon BM, Fu YM, Casscells W, Epstein SE. Smooth muscle cell abundance and fibroblast growth factors in coronary lesions of patients with non fatal unstable angina: a clue to the mechanism of transformation from the stable to the unstable clinical state. Circulation. 1993;88:2493–2500.
Serruys PW, de Jaegere P, Kiemeneij, et al. A comparison of balloon expandable-stent implantation with balloon angioplasty in patients with coronary artery disease. N Engl J Med. 1994;331:489–495.
Fischman DL, Leon MB, Baim DS, et al. A randomized comparison of coronary-stent placement and balloon angioplasty in the treatment of coronary artery disease. N Engl J Med. 1994;331:496–501.
Flugelman MY, Jaklitsch MT, Newman KD, Casscells SW, Bratthuaer GL, Dichek DA. Low levels in vivo gene transfer into the arterial wall through a perforated balloon catheter. Circulation. 1992;85:1110–1117.
Coghlan A. Gene superclub signs up top players. New Scientist. 1994;1952:4.
Banai S, Shou M, Correa R, Jaklitsch MT, Douek PC, Booner RF, Epstein SE, Unger EF. Rabit ear model of injury induced arterial smooth muscle cell proliferation. Circ Res. 1991;69:748–756.
Lee SW, Trapnell BC, Rade JJ, Virmani R, Dichek DA. in vivo adenoviral vector-mediated gene transfer into balloon-injured rat carotid arteries. Circ Res. 1993;73:797–807.
Schulick AH, newman KD, Dichek DA. A therapeutic window for in vivo adenoviral vector mediated gene transfer. Circulation 1994;90(Supl 4):I–516.
Reissen R, Isner JM. Prospects for site-specific delivery of pharmacologic and molecular therapies. J Am Coll Cardiol. 1994;23:1234–1244.
Plante S, Dupuis G, Mongeau CJ, Durand P. Porous balloon catheters for local delivery: assessment of vascular damage in a rabbit iliac angioplasty model. J Am Coll Cardiol. 1994;24:820–824.
Feldman LJ, Steg PG, Zheng LP, Kearney M,Barry JJ, Perricaudet M, Isner JM. Percutaneous adeno-mediated gene delivery to normal and atherosclerotic arteries in vivo: a comparative study. Circulation. 1994;90(Supl 4):I–517.
Shweiki D, Itin A, soffer D, Keshet E. Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature. 1992;359:843–845.
Ferrara N, Houck K A, Jakeman L B, Winer J, Leung DW. The vascular endothelial growth factor family of polypeptides. J Cell Biochem. 1991;47:211–218.
Ohno T, Gordon D, San H, Pompili VJ, Imperiale MJ, Nabel GJ, Nabel EG. Gene therapy for vascular smooth muscle cell proliferation after arterial injury. Science. 1994;265:781–84.
Morishita R, Gibbons GH, Ellison KE, Nakajima Masatoshi, von der Leyen H, Zhang L, Kaneda Y, Ogihara T, Dzau VJ. Intimal hyperplasia after vascular injury is inhibited by antisense cdk 2 kinase oligonucleotides. J Clin Invest. 1994;93:1458–1464.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Flugelman, M.Y. (1995). Vascular Gene Therapy. In: Sideman, S., Beyar, R. (eds) Molecular and Subcellular Cardiology. Advances in Experimental Medicine and Biology, vol 382. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1893-8_27
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1893-8_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5772-8
Online ISBN: 978-1-4615-1893-8
eBook Packages: Springer Book Archive