Isolation and Analysis of Genomic Sequences from Mycorrhizal Fungi
The isolation of effective probes is of paramount importance in studies aimed at elucidating the molecular mechanisms underlying the development of ectomycorrhizal fungi, as well as in the creation of biotechnological tools for improving truffle harvest and species identification. In one strategy which we are using to isolate DNA probes, recombinant plasmids are randomly isolated from genomic libraries and their nucleotide sequences compared with available data banks of nucleotide and aminoacid sequences. As a first step in this strategy, we have analyzed 56 clones from a genomic library of plasmids bearing small inserts of DNA from Tuber albidum. Several clones were singled out on the basis of sequence similarity with known genes. Among these was a recombinant plasmid carrying nucleotide sequences potentially coding for a protein extremely similar to the a subunit of Fatty Acid Synthase of other fungal species. The potentially coding region appeared to be interrupted by an intron and its sequence has allowed the construction of an evolutionary tree including Tuber magnatum and three different fungal species.
KeywordsCodon Agarose Polypeptide Assimilation dNTP
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