Cytotoxic Effects of Antifungal Drugs on Cultured Human Conjunctival Cells
We evaluated the cytotoxicity of 3 antifungal drugs using cultured human conjunctival cells. Cell growth curve, electron microscopic appearance and analysis of DNA histograms were studied after 2 to 4 min exposures to the test solution. Amphotericin B inhibited cell growth dose-dependently. Amphotericin B from 50 µg/ml to 100 µg/ml revealed loss of microvilli and a remarkable decrease in cell population. Miconazole (10 mg/ml) destroyed the cells completely. Fluconazole, at a concentration usually used (1 mg/ml), did not cause inhibition of cell growth or damage to the cell surface. None of the drugs studied resulted in abnormal pattern on DNA histograms.
KeywordsOcular Surface Antifungal Drug Ophthalmic Solution Abnormal Pattern Benzalkonium Chloride
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