Cytotoxic Effects of Antifungal Drugs on Cultured Human Conjunctival Cells
We evaluated the cytotoxicity of 3 antifungal drugs using cultured human conjunctival cells. Cell growth curve, electron microscopic appearance and analysis of DNA histograms were studied after 2 to 4 min exposures to the test solution. Amphotericin B inhibited cell growth dose-dependently. Amphotericin B from 50 µg/ml to 100 µg/ml revealed loss of microvilli and a remarkable decrease in cell population. Miconazole (10 mg/ml) destroyed the cells completely. Fluconazole, at a concentration usually used (1 mg/ml), did not cause inhibition of cell growth or damage to the cell surface. None of the drugs studied resulted in abnormal pattern on DNA histograms.
KeywordsPermeability Toxicity Dioxide Iodide Dehydration
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