Non-Mammalian Vertebrate Pepsinogens and Pepsins: Isolation and Characterization
Pepsinogen, the precursor of pepsin, is secreted from the gastric mucosa into the gastric lumen, where it is autocatalytically activated to pepsin under acidic conditions. It is classified into three major types, that is, pepsinogen A, pepsinogen C (or progastricsin), and prochymosin (or neonatal pepsinogen). Further, pepsinogens, especially pepsinogens A, are often composed of several isozymogens. So far, a number of pepsinogens were purified from various sources, characterized and sequenced at the protein level and/or DNA level. Thus the complete amino acid sequences of mammalian pepsinogens are known for human (1), monkey (2), porcine (3–6), and rabbit (7) pepsinogens A, human (8), monkey (9), rat (10) and guinea pig (11) pepsinogens C, and bovine prochymosin (12).
KeywordsCellulose Filtration Carbohydrate Propane Fractionation
Unable to display preview. Download preview PDF.
- 10.T. Ishihara, Y. Ichihara, T. Hayano. I. Katsura, K. Sogwa, Y Fujii-Kuriyama and K. Takahashi, Primary Strucutre and Transcriptional Regulation of Rat Pepsinogen C. Gene, J. Biol. Chem. 264: 10193–10199(1989).Google Scholar
- 11.T. Kageyama. M. Ichinose, S. Tsukada, K. Miki, K. Kurokawa, O. Koiwai, M. Tanji, E. Yakabe, S. B. P. Athauda and K. Takahashi, Gastric Prochathepsin E and Progastricsin from Guinea Pig. Purification, Molecular Cloning of cDNAs, and Characterization of Enzymatic Properties, with Special Reference to Procathepsin E, J. Biol. Chem. 267: 16450–16459 (1992).PubMedGoogle Scholar
- 17.E. Yakabe, M. Tanji, M. Ichinose, S. Goto, K. Miki, K. Kurokawa, H. Ito, T. Kageyama and K. Takahashi, Purification, Characterization, and Amino Acid Sequences of Pepsinogens and Pepsins from the Esophageal Mucosa of Bullfrog (Rana catesbeiana), J. Biol Chem. 266: 22436–22443 (1991).PubMedGoogle Scholar
- 19.S. B. P. Athauda, M. Tanji, T. Kageyama and K. Takahashi, A Comparative Study on the NH2-Terminal Amino Acid Sequences and Some Other Properties of Six Isozymic Forms of Human Pepsinogens and Pepsins, J. Biol Chem. 106: 920–927 (1989).Google Scholar
- 22.K. Takahashi and T. Kageyama, Multiplicity and Intermediates of the Activation Mechanism of Zymogens of Gastric Aspartic Proteinases, in: “Aspartic Proteinases and Their Inhibitors”, V. Kostka, ed., pp.265–282, Walter de Gruyter, Berlin and New York (1985).Google Scholar
- 23.O. Matsuzaki and K. Takahashi, Improved Purification of Slow Moving Protease from Human Gastric Mucosa and Its Action on the B Chain of Oxidized Bovine Insulin, Biomed Res. 9: 515–523 (1988).Google Scholar
- 32.B. Foltmann and V B. Pedersen, Comparison of the Primary Structures of Acidic Proteinases and of Their Zymogens, in: “Acid Proteinases” J. Tang, ed., pp. 3–22, Plenum Press, New York (1977).Google Scholar