Cell Trafficking Networks in Cutaneous T Cell Lymphoma
An extensive network of Thy-1+/Factor XIIIa+ dermal dendrocytes (DD) (Table 1) exists within the papillary dermis in lesions of cutaneous T cell lymphoma (CTCL). To further characterize these cells and to study the role that activation signals and adhesion molecules play in the cellular dynamics of CTCL, immunohistochemical staining of formalin fixed, paraffin embedded tissue sections was used to study expression of high endothelial cell antigen-1 (HECA-452) and proliferating cell nuclear antigen (PCNA) in lesions of CTCL as compared to those of psoriasis, Rhus dermatitis and normal skin. In diseased skin, 10–60% of epidermotropic lymphocytes were PCNA+, comparable to the numbers of Ki-67+ and human mucosal leukocyte antigen (HML)-1+ cells seen in previous studies. HECA-452 expression by dermal lymphocytes ranged from 1–42%. Only in lesions of CTCL were appreciable numbers of epidermal HECA-452+ lymphocytes present (mean = 31%+). PCNA+ lymphocytes were routinely seen only in CTCL and were limited to a subset of cells within the epidermis.
KeywordsProliferate Cell Nuclear Antigen Factor XIIIa Allergic Contact Dermatitis Mycosis Fungoides Papillary Dermis
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- Duijvestin, AM, E Horst, ST Pals, BN Rouse, AC Steere, LJ Picker, CJLM Meijer and EC Butcher, 1988. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol., 130: 147–155.Google Scholar
- Fivenson, DP, RW Dunstan, MC Douglass, BJ Nickoloff and PF Moore, 1991b. Thy-1+ dermal dendrocytes in mycosis fungoides. J. Invest. Dermatol., 96: 599A.Google Scholar
- Hansen, ER, O Baadsgaard, S Lisby, KD Cooper, K Thomsen and GL Vejlsgaard, 1990. Cutaneous T cell lymphoma lesionai epidermal cells activate autologous CD8+ T lymphocytes: Involvement of both CD1+, OKM5+ and CD1+, OKM5 antigen-presenting cells. J. Invest. Dermatol., 94: 485–491.PubMedCrossRefGoogle Scholar
- Headington, JT, 1986. The dermal dendrocyte. In Callen JP, Dahl MV, Golitz LE et al. Eds: Advances in Dermatology, Vol. 1, Chicago: Year Book Medical Publishers Inc., 159–171.Google Scholar
- Nguyen, C, M-G Mattei, J-F Mattei, MJ Santoni, C Goridis and BR Jordan, 1986. Localization of human N-CAM gene band to q23 of chromosome 11: The third gene coding for a cell interaction molecule mapped to the distal portion of the long arm of chromosome 11. J. Cell Biol., 102: 711–715.PubMedCrossRefGoogle Scholar
- Nickoloff, BJ, DP Fivenson, CEM Griffiths and R Modlin, 1991. Preferential but not unique cutaneous endothelial cell (EC) expression of ELAM-1. Clin. Res., 39: 153A.Google Scholar
- Nickoloff, BJ and CEM Griffiths, 1990b. Lymphocyte trafficking in psoriasis: A new perspective emphasizing the dermal dendrocyte with active dermal recruitment medicated via endothelial cells followed by intra-epidermal T cell activation. J. Invest. Dennatol., 95: 355–375.Google Scholar