Abstract
The aim of this study was to evaluate the expression on neoplastic cell membranes of some receptors involved in intercellular and cell-matrix adhesion. We used monoclonal antibodies (mAbs) directed against VLA-3, VLA-4, LFA-1, ICAM-1, Leu-8 and CD44 antigens, with an alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemical method, in 40 cases of cutaneous B-cell lymphomas (CBCL) and 60 cases of cutaneous T-cell lymphomas (CTCL), which had been previously characterized employing a large panel of mAbs. Our results showed, in CBCL, consistent expression of CD44, CDlla, ICAM-1 and VLA-4 molecules. LEU-8 and VLA-3 receptors were detected, respectively, in 50% and in 25% of the cases examined. No significant difference was noted between the pattern of adhesion molecules of our CBCL and of 70 cases of nodal follicular lymphomas. In CTCL, CD44 reactivity was demonstrated in all cases; VLA-4, CDlla and ICAM-1 were negative in a case of γδ T-cell lymphoma and in a case of highly malignant CD8+ lymphoma. Furthermore, anti-VLA-4 mAb did not label a case with an NK-like phenotype, nor a case of Ki-1+ lymphoma. Interestingly anti-VLA-3 mAb, normally weakly expressed on T-cell membranes, was strongly positive on blastic cells with an activated phenotype, particularly in Ki-1+ lymphomas, including lymphomatoid papulosis. Reed-Sternberg cells in 5 cases of Hodgkin disease examined were VLA-2 negative. VLA-3 receptor seems to be involved not only in cell-matrix and cell-cell adhesion, but also in lymphocyte activation.
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References
Albelda SM and Buck CA: Integrins and other cell adhesion molecules. FASEB J, 4: 2868–2880, (1990).
Berti A, A Cerri, S Cavicchini, D Delia, D Soligo, E Alessi and R Caputo, 1991. Primary cutaneous gamma-delta T-cell lymphoma presenting as disseminated pagetoid retiulosis. J. Invest. Dermatol., 96(5): 718–723.
Berti E, E Alessi and R Caputo, 1991. Reticulohistiocytoma of the dorsum (Crosti’s disease) and other B-cell lymphomas. Seminar in diagnostic pathology, 8(2): 82–90.
Berti E, R Gianotti, E Alesis and R Caputo, 1989. Primary cutaneous follicular center cell lymphoma: Immunophenotypical and immunogenotypical aspects. Curr. Probi. Dermatol., 19: 196–202.
Butcher EC, 1990. Cellular and molecular mechanism that direct leukocyte traffic. Am. J. Pathol., 136(1).
Camerini D, SP James, I Stamenkovic and B Seed, 1989. Leu8/TQ1 is the human equivalent of the MEL-14 lymph node homing receptor. Nature, 342: 78–82.
Elices MJ, LA Urry and ME Hemler, 1991. Receptor functions for the Integrin VLA3: Fibronectin, Collagen, and Laminin binding are differentially influenced by ARG-GLY-ASP peptide and by divalent cations. J. Cell Biol., 112(1): 169–181.
Haynes BF, M Telen, L Hale and S Denning, 1989. CD44: A molecule involved in leukocyte adherence and T-cell activation. Immunology Today, 10(12): 423–428.
Hynes RO, 1987. Integrins: A family of cell surface receptors. Cell, 48: 549–554.
Hynes RO, 1992. Integrins: Versatility, modulation, and signalling in cell adhesion. Cell, 69: 11–25.
Jalkanen S, H Joensuu, KO Soderstrom and P Klemi, 1991. Lymphocyte homing and clinical behavior of Non-Hodgkin’s lymphoma. J. Clin. Invest., 87: 1835–1840.
Makgoba MW, ME Sanders and S Shaw, 1989. The CD2-LFA3 and LFA-l-ICAM pathways: Relevance to T-cell recognition. Immunology Today, 10(12): 417–422.
Pals ST, CJ Meijer and T Radaszkiewicz, 1991. Expression of the human peripheral lymph node homing receptor (LECAM-1) in nodal and gastrointestinal non-Hodgkin’s lymphomas. Leukemia, 5(7): 628–631.
Picker L, J Medeiros, L Weiss, R Warnke and EC Butcher, 1988. Expression of lymphocyte homing receptor antigen in Non-Hodgkin’s Lymphoma. Am. J. Pathol., 130(3): 496–504.
Roossien FF, D de Rijk, A Bikker and E Roos, 1989. Involvement of LFA-1 in lymphoma invasion and metastasis demonstrated with LFA-1-deficient mutants. J. Cell Biol., 108: 1979-198.
Schwartz MA, 1992. Transmembrane signalling by integrins. Trends Cell Biol., 2: 304–308.
Sun Sy M, YJ Guo and I Stamenkovic, 1991. Distinct effects of two CD44 isoforms on tumor growth in vivo. J. Exp. Med., 174: 859–866.
Takada Y, EA Wayner, WG Carter and ME Hemler, 1988. Extracutaneous matrix receptors, ECMRII and ECM RI, for collagen and fibronectin, correspond to VLA-2 and VLA-3 in the VLA family of heterodimers. J. Cell Biochem., 37: 385–393.
Wayuner EA, WG Carter, RS Piotrowica and TJ Kunicki, 1988. The function of exracellular matrix receptors in mediating cell adhesion to extracellular matrix: Preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa. J. Cell Biol., 107: 1881–1891.
Willemze R, CJLM Meijer, HJ Sentis, E Scheffer, WA van Vloten, J Toonstra and C Van Der Putte, 1981. Primary cutaneous large cell lymphomas of follicular center cell origin. J. Am. Acad. Dermatol., 16: 518–526.
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© 1994 Springer Science+Business Media New York
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Berti, E., Randi, P., Roscetti, E., Delia, D. (1994). Integrins and Homing Receptors in Cutaneous Lymphomas. In: Lambert, W.C., Giannotti, B., van Vloten, W.A. (eds) Basic Mechanisms of Physiologic and Aberrant Lymphoproliferation in the Skin. NATO ASI Series, vol 265. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1861-7_11
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DOI: https://doi.org/10.1007/978-1-4615-1861-7_11
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