Evaluation of In Vivo Free Radical Activity during Endotoxic Shock Using Scavengers, Electron Microscopy, Spin Traps, and Electron Paramagnetic Resonance Spectroscopy
Controversy exists concerning the involvement of free radical activity during sepsis and endotoxic shock as evidence has accumulated that both implicate and eliminate free radicals in the pathophysiologic events that occur during these disease processes. The majority of these data are from studies utilizing scavengers or inhibitors of lipid peroxidation to attenuate the activity resulting from excessive free radical generation and as such represent only indirect evidence of the presence of free radicals. This study employed methodology combining spin trapping techniques with electron paramagnetic resonance spectroscopy1 to directly verify the presence of in vivo free radical generation in the conscious endotoxic rat.2 Additionally, myocardial structure has been evaluated using transmission electron microscopy to assess the effect of a hydroxyl radical scavenger on endotoxin-induced injury to the myocardium.3
KeywordsElectron Paramagnetic Resonance Free Radical Generation Electron Paramagnetic Resonance Spectroscopy Hydroxyl Radical Scavenger Endotoxic Shock
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- 1.P.B. McCay and J.L. Poyer, General mechanisms of spin trapping in vitro and in vivo, in: “Handbook of Free Radicals and Antioxidants in Biomedicine” p187, CRC Press, Boca Raton.Google Scholar
- 3.D.L. Stiers, D.J. Brackett, and M.F. Wilson, Dimethyl sulfoxide protection of endotoxin-induced myocardial injury, in: “Proceedings of the 46th Annual Meeting of the Electron Microscopy Society of America” p 320, G.W. Bailey, ed., San Francisco Press, Inc., San Francisco (1988).Google Scholar