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The NIMA kinase: A mitotic regulator in Aspergillus nidulans and vertebrate cells

  • Kun Ping Lu
  • Tony Hunter
Part of the Progress in Cell Cycle Research book series (PCCR)

Abstract

CDC2 has been shown to regulate entry into mitosis in eukaryotic cells. However, in Aspergillus nidulans, activation of CDC2 itself is not sufficient to trigger mitosis if another mitotic protein kinase, NIMA, is not activated. Superficially, NIMA and CDC2 have analogous functions and are regulated in a similar manner. NIMA activity is tightly regulated during the cell cycle. Overexpression of NIMA induces germinal vesicle breakdown in Xenopus oocytes and promotes premature entry into mitosis in all eukaryotic cells examined, whereas dominant-negative mutant NIMA causes a specific G2 arrest in Aspergillus nidulans and human cells, as is the case for CDC2. However, NIMA and CDC2 have quite distinct primary sequence substrate specificities. Furthermore, the regulatory mechanisms that govern the cell cycle-dependent abundance, activity and localization are largely intramolecular for NIMA but intermolecular for CDC2. More importantly, a NIMA-like pathway is also required for the G2/M transition in vertebrate cells. Thus, NIMA may represent a new essential eukaryotic cell cycle regulator, although its homologues in other species are yet to be identified.

Keywords

HeLa Cell Xenopus Oocyte Oocyte Maturation Okadaic Acid Aspergillus Nidulans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Nurse, P. (1994) Cell 79, 547–550.PubMedCrossRefGoogle Scholar
  2. 2.
    Heichman, K.A. and Roberts, J.M. (1994) Cell 79, 557–562.PubMedCrossRefGoogle Scholar
  3. 3.
    King, R.W., Jackson, P.K. and Kirschner, M.W. (1994) Cell 79, 563–571.PubMedCrossRefGoogle Scholar
  4. 4.
    Sorger, P.K. and Murray, A.W. (1992) Nature 355, 365–368.PubMedCrossRefGoogle Scholar
  5. 5.
    Amon, A., Surana, U., Muroff, I. and Nasmyth, K. (1992) Nature 355, 368–371.PubMedCrossRefGoogle Scholar
  6. 6.
    Stueland, C.S., Lew, D.J., Cismowski, M.J. and Reed, S.I. (1993) Mol. Cell. Biol. 13, 3744–3755.PubMedGoogle Scholar
  7. 7.
    Osmani, A.H., McGuire, S.L. and Osmani, S.A. (1991) Cell 67, 283–291.PubMedCrossRefGoogle Scholar
  8. 8.
    Choi, T., Aoki, F., Mori, M., Yamashita, M., Nagahama, Y. and Kohmoto, K. (1991) Development 113, 789–795.PubMedGoogle Scholar
  9. 9.
    Jung, T., Moor, R.M. and Fulka, J.J. (1993) Int. J. Dev. Biol. 37, 595–600.PubMedGoogle Scholar
  10. 10.
    Gavin, A.C., Cavadore, J.C. and Schorderet, S.S. (1994) J. Cell Sci. 275–283.Google Scholar
  11. 11.
    Osmani, S.A., Pu, R.T. and Morris, N.R. (1988) Cell 53, 237–244.PubMedCrossRefGoogle Scholar
  12. 12.
    Osmani, S.A., May, G.S. and Morris, N.R. (1987) J. Cell Biol. 104, 1495–1504.PubMedCrossRefGoogle Scholar
  13. 13.
    Osmani, A.H., O’Donnell, K., Pu, R.T. and Osmani, S.A. (1991) EMBO J. 10, 2669–2679.PubMedGoogle Scholar
  14. 14.
    Lu, K.P. and Means, A.R. (1994) EMBO J. 13, 2103–2113.PubMedGoogle Scholar
  15. 15.
    Lu, K.P., Osmani, S.A. and Means, A.R. (1993) J. Biol. Chem. 268, 8769–8776.PubMedGoogle Scholar
  16. 16.
    Lu, K.P., Kemp, B.E. and Means, A.R. (1994) J. Biol. Chem. 269, 6603–6607.PubMedGoogle Scholar
  17. 17.
    Songyang, Z., Lu, K.P., Blechner, S., Filhol, O., Cochet, C., Soderling, T.R., Bartleson, C., Graves, D.J., Blenis, J., Hunter, T. and Cantley, L.C. (1995) manuscript submitted.Google Scholar
  18. 18.
    Nigg, E.A. (1993) Curr. Opin. Cell Biol. 5, 187–193.PubMedCrossRefGoogle Scholar
  19. 19.
    Lu, K.P. and Hunter, T. (1995) Cell 81, 413–424.PubMedCrossRefGoogle Scholar
  20. 20.
    Timberlake, W.E. and Marshall, M.A. (1989) Science 244, 1313–1317.PubMedCrossRefGoogle Scholar
  21. 21.
    Waring, R.B., May, G.S. and Morris, N.R. (1989) Gene 79, 119–130.PubMedCrossRefGoogle Scholar
  22. 22.
    Lu, K.P. and Means, A.R. (1992) Methods Mol. Genet. 2, 255–275.Google Scholar
  23. 23.
    Morris, N.R., Osmani, S.A., Engle, D.B. and Doonan, J.H. (1989) Bioessays 10, 196–201.PubMedCrossRefGoogle Scholar
  24. 24.
    Bergen, L.G. and Morris, N.R. (1983) J. Bacteriol. 156, 155–160.PubMedGoogle Scholar
  25. 25.
    Morris, N.R. (1976) Genet. Res. 26, 237–254.CrossRefGoogle Scholar
  26. 26.
    O’Connell, M.J., Osmani, A.H., Morris, N.R. and Osmani, S.A. (1992) EMBO J. 11, 2139–2149.Google Scholar
  27. 27.
    Osmani, A.H., van Peji, N., Mischke, M., O’Connell, M.J. and Osmani, S.A. (1994) J. Cell Sci. 107, 1519–1528.PubMedGoogle Scholar
  28. 28.
    Lu, K.P. and Means, A.R. (1993) Endo. Rev. 14, 40–58.Google Scholar
  29. 29.
    Oakley, B.R. and Morris, N.R. (1983) J. Cell Biol. 96, 1155–1158.PubMedCrossRefGoogle Scholar
  30. 30.
    Osmani, S.A., Eagle, D.B., Doonan, J.H. and Morris, N.R. (1988) Cell 52, 241–251.PubMedCrossRefGoogle Scholar
  31. 31.
    Hanks, S.K., Quinn, A.M. and Hunter, T. (1988) Science 241, 42–52.PubMedCrossRefGoogle Scholar
  32. 32.
    O’Connell, M.J., Norbury, C. and Nurse, P. (1994) EMBO J. 13, 4926–4937.Google Scholar
  33. 33.
    Songyang, Z., Blechner, S., Piwnica-Worms, H. and Cantley, L.C. (1994) Curr. Biol. 4, 973–982.PubMedCrossRefGoogle Scholar
  34. 34.
    Draetta, G. (1993) Trends Cell Biol. 3, 287–289.PubMedCrossRefGoogle Scholar
  35. 35.
    Rossomando, A.J., Wu, J., Michel, H., Shabanowitz, J., Hunt, D.F., Weber, M.J. and Sturgill, T.W. (1992) Proc. Natl. Acad. Sci. USA 89, 5779–5783.PubMedCrossRefGoogle Scholar
  36. 36.
    Anderson, N.G., Maller, J.L., Tonks, N.K. and Sturgill, T.W. (1990) Nature 343, 651–653.PubMedCrossRefGoogle Scholar
  37. 37.
    Marshall, C.J. (1994) Curr. Opin. Genet. Dev. 4, 82–89.PubMedCrossRefGoogle Scholar
  38. 38.
    Lu, K.P. and Means, A.R. (1995) Adv. Mol. Cell. Biol. (In press).Google Scholar
  39. 39.
    Lu, K.P., Osmani, S.A., Osmani, A.H. and Means, A.R. (1993) J. Cell Biol. 121, 621–630.PubMedCrossRefGoogle Scholar
  40. 40.
    Swenson, K.I., Farell, K.M. and Ruderman, J.V. (1986) Cell 47, 861–870.PubMedCrossRefGoogle Scholar
  41. 41.
    Murray, A.W. and Kirschner, M.W. (1989) Science 246, 614–621.PubMedCrossRefGoogle Scholar
  42. 42.
    Yew, N., Strobel, M. and Vande Woude, G.F. (1993) Curr. Opin. Genet. Dev. 3, 19–25.PubMedCrossRefGoogle Scholar
  43. 43.
    Maller, J.L. (1993) Mol. Cell Biochem. 128, 267–281.CrossRefGoogle Scholar
  44. 44.
    Norbury, C., Blow, J. and Nurse, P. (1991) EMBO J. 10, 3321–3329.PubMedGoogle Scholar
  45. 45.
    Krek, W. and Nigg, E.A. (1991) EMBO J. 10, 3331–3341.PubMedGoogle Scholar
  46. 46.
    Pickham, K.M., Meyer, A.N., Li, J. and Donoghue, D.J. (1992) Mol. Cell. Biol. 12, 3192–3203.PubMedGoogle Scholar
  47. 47.
    Muramatsu, M., Kaibuchi, K. and Arai, K. (1989) Mol. Cell. Biol. 9, 831–836.PubMedGoogle Scholar
  48. 48.
    Waldmann, R., Hanson, P.I. and Schulman, H. (1990) Biochemistry 29, 1679–1684.PubMedCrossRefGoogle Scholar
  49. 49.
    Kuang, J., Zhao, J.Y., Wright, D.A., Saunders, G.F. and Rao, P.N. (1989) Proc. Natl. Acad. Sci. USA 86, 4982–4986.PubMedCrossRefGoogle Scholar
  50. 50.
    Gavin, A.C., Vassalli, J.D., Cavadore, J.C. and Schorderet, S.S. (1992) Mol. Reprod. Dev. 33, 287–296.PubMedCrossRefGoogle Scholar
  51. 51.
    Sagata, N., Oskarsson, M., Copeland, T., Brumbaugh, J. and Vande, W.G. (1988) Nature 335, 519–525.PubMedCrossRefGoogle Scholar
  52. 52.
    Freeman, R.S., Kanki, J.P., Ballantyne, S.M., Pickham, K.M. and Donoghue, D.J. (1990) J. Cell Biol. 111, 533–541.PubMedCrossRefGoogle Scholar
  53. 53.
    Mailer, J.L. and Smith, D.S. (1985) Dev. Biol. 109, 150–156.CrossRefGoogle Scholar
  54. 54.
    Gossen, M. and Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547–5551.PubMedCrossRefGoogle Scholar
  55. 55.
    Eldredge, E.R., Chiao, P.J. and Lu, K.P. (1994) Meth. Enzymol. (In press).Google Scholar
  56. 56.
    Johnson, R.T. and Rao, P.N. (1970) Nature 226, 717–722.PubMedCrossRefGoogle Scholar
  57. 57.
    Kalderon, D., Roberts, B.L., Richardson, W.D. and Smith, A.E. (1984) Cell 39, 499–509.PubMedCrossRefGoogle Scholar
  58. 58.
    Matsumura, F. and Yamashiro, S. (1993) Curr. Opin. Cell Biol. 5, 70–76.PubMedCrossRefGoogle Scholar
  59. 59.
    Korn, E.D. and Hammer, J.A.I. (1988) Annu Rev Biophys Biophys Chem 17, 23–45.PubMedCrossRefGoogle Scholar
  60. 60.
    Titus, M.A. (1993) Curr. Opin. Cell Biol. 5, 77–81.PubMedCrossRefGoogle Scholar
  61. 61.
    Fleig, U.N., Gould, K.L. and Nurse, P. (1992) Mol. Cell. Biol. 12, 2295–2301.PubMedGoogle Scholar
  62. 62.
    Fleig, U.N. and Nurse, P. (1991) Mol. Gen. Gene.t 226, 432–440.Google Scholar
  63. 63.
    Pines, J. and Hunter, T. (1991) J. Cell Biol. 115, 1–17.PubMedCrossRefGoogle Scholar
  64. 64.
    Pines, J. and Hunter, T. (1994) EMBO J. 13, 3772–3781.PubMedGoogle Scholar
  65. 65.
    Parge, H.E., Arvai, A.S., Murtari, D.J., Reed, S.I. and Tainer, J.A. (1993) Science 262, 387–395.PubMedCrossRefGoogle Scholar
  66. 66.
    Kitagawa, M., Okabe, T., Ogino, H., Matsumoto, H., Suzuki-Takahashi, I., Kokubo, T., Higashi, H., Saitoh, S., Taya, Y., Yasuda, H., Ohba, Y., Nishimura, S., Tanaka, N. and Okuyama, A. (1993) Oncogene 8, 2425–2432.PubMedGoogle Scholar
  67. 67.
    Kitagawa, M., Higashi, H., Takahashi, S.I., Okabe, T., Ogino, H., Taya, Y., Nishimura, S. and Okuyama, A. (1994) Oncogene 9, 2549–2557.PubMedGoogle Scholar
  68. 68.
    Gale, M.J. and Parsons, M. (1993) Mol. Biochem. Parasitol. 59, 111–121.PubMedCrossRefGoogle Scholar
  69. 69.
    Baron, A.B., Davies, C.J., Hutchison, C.A. and Kabak, D.B. (1992) Gene 117, 137–140.CrossRefGoogle Scholar
  70. 70.
    Schweitzer, B. and Philippsen, P. (1992) Mol. Gen. Genet. 234, 164–167.PubMedGoogle Scholar
  71. 71.
    Letwin, K., Mizzen, L., Motro, B., Ben, D.Y., Bernstein, A. and Pawson, T. (1992) EMBO J. 10, 3521–3531.Google Scholar
  72. 72.
    Schultz, S.J. and Nigg, E.A. (1993) Cell Growth Differ. 4, 821–830.PubMedGoogle Scholar
  73. 73.
    Schultz, S.J., Fry, A.M., Sutterlin, C., Reid, T. and Nigg, E.A. (1994) Cell Growth Diff. 5, 625–635.PubMedGoogle Scholar
  74. 74.
    Levedakou, E.N., He, M., Baptist, E.W., Craven, R.J., Cance, W.G., Welcsh, P.L., Simmons, A., Naylor, S.L., Leach, R.J., Lewis, T.B., Bowcock, A. and Liu, E. T. (1994) Oncogene 9, 1977–1988.PubMedGoogle Scholar
  75. 75.
    Pines, J. and Hunter, T. (1995) in The Cell Cycle: Frontiers in Molecular Biology (Glover, D.M. and Hutchison, C., eds.) Oxford Univerisity Press, Oxford (in press).Google Scholar
  76. 76.
    Ye, X.S., Xe, G., Pu, R.T., Fincher, R.R., McGuire, S.L., Osmani, A.H. and Osmani, S.A. (1995) EMBO J. 14, 986–994.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Kun Ping Lu
    • 1
  • Tony Hunter
    • 1
  1. 1.Molecular Biology and Virology LaboratoryThe Salk InstituteLa JollaUSA

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