Abstract
More than 13 reactions are catalyzed by corrinoid-containing enzymes in prokaryotes and eukaryotes. These reactions were reviewed previously (Stadtman, 1971; Halpern, 1985) but some additional corrinoid-dependent reactions have been discovered since then. For example, the methanol conversion into methane proceeds via a corrinoid-dependent methyltransferase (van der Meijden et al., 1984a). The enzyme from Methanosarcina barkeri provides the methyl group from methanol to the specific methanogenic cofactor 2-mercaptoethanesulfonic acid (HS-CoM) after the protein is reductively activated by H2 and ATP. That enzyme revealed an α2β structure and it contained 3–4 mol loosely bound corrinoid per mole of protein. In vitro studies with mixed cell-free extracts of Methanosarcina barkeri and Eubacterium limosum indicated that the acetogenic bacterium also possesses a methylated corrinoid enzyme. This enzyme was demethylated in the presence of methylcobalamin: coenzyme M methyltransferase from the methanogenic bacterium with concomitant formation of methyl-coenzyme M (van der Meijden et al., 1984b).
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Stupperich, E. (1994). Corrinoid-Dependent Mechanism of Acetogenesis from Methanol. In: Drake, H.L. (eds) Acetogenesis. Chapman & Hall Microbiology Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1777-1_6
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DOI: https://doi.org/10.1007/978-1-4615-1777-1_6
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