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Protein Kinase CK2 — From Structure to Regulation pp 113–117Cite as

Mutation at the CK2 phosphorylation site on Cdc28 affects kinase activity and cell size in Saccharomyces cerevisiae

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Part of the book series: Molecular and Cellular Biochemistry ((DMCB,volume 35))

Abstract

We have recently reported that protein kinase CK2 phosphorylates both in vivo and in vitro residue serine-46 of the cell cycle regulating protein Cdc28 of budding yeast Saccharomyces cerevisiae,confirming a previous observation that the same site is phosphorylated in Cdc2/Cdkl, the human homolog of Cdc28. In addition, S. cerevisiae in which serine-46 of Cdc28 has been mutated to alanine show a decrease of 33% in both cell volume and protein content, providing the genetic evidence that CK2 is involved in the regulation of budding yeast cell division cycle, and suggesting that this regulation may be brought about in G1 phase of the mammalian cell cycle. Here, we extended this observation reporting that the mutation of serine-46 of Cdc28 to glutamic acid doubles, at least in vitro, the H1-kinase activity of the Cdc28/cyclin A complex. Since this mutation has only little effects on the cell size of the cells, we hypothesize multiple roles of yeast CK2 in regulating the G1 transition in budding yeast. (Mol Cell Biochem 227: 113-117,2001)

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Author information

Authors and Affiliations

  1. Istituto di Scienze dell’Alimentazione, Consiglio Nazionale delle Ricerche, Avellino, Italy

    Gian Luigi Russo

  2. Martek Biosciences Inc., Columbia, MD, USA

    Christian van den Bos

  3. Cambrex Biosciences Group, Walkersville, MD, USA

    Daniel R. Marshak

Authors
  1. Gian Luigi Russo
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  2. Christian van den Bos
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  3. Daniel R. Marshak
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Editor information

Editors and Affiliations

  1. Cellular and Molecular Biochemistry Research Lab, University of Minnesota, Veterans Affairs Medical Center, One Veterans Drive, Minneapolis, 55417, MN, USA

    Khalil Ahmed

  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark

    Olaf-Georg Issinger

  3. Program de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, 70086, Casilla, Santiago 7, Chile

    Jorge E Allende

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© 2001 Springer Science+Business Media New York

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Russo, G.L., van den Bos, C., Marshak, D.R. (2001). Mutation at the CK2 phosphorylation site on Cdc28 affects kinase activity and cell size in Saccharomyces cerevisiae . In: Ahmed, K., Issinger, OG., Allende, J.E. (eds) Protein Kinase CK2 — From Structure to Regulation. Molecular and Cellular Biochemistry, vol 35. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1723-8_14

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  • DOI: https://doi.org/10.1007/978-1-4615-1723-8_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5696-7

  • Online ISBN: 978-1-4615-1723-8

  • eBook Packages: Springer Book Archive

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