A gene located at 72A in Drosophila melanogaster encodes a novel zinc-finger protein that interacts with protein kinase CK2

  • Madhavi Kalive
  • Regina L. Trott
  • Ashok P. Bidwai
Part of the Molecular and Cellular Biochemistry book series (DMCB, volume 35)


Drosophila melanogaster protein kinase CK2 (DmCK2) is a Ser/Thr protein kinase composed of catalytic α and regulatory β subunits associated as an α2β2 heterotetramer. Using the two hybrid system, we have screened a Drosophila embryo cDNA library in order to identify proteins that interact with DmCK2α. One of these cDNAs encodes a novel previously undescribed zinc-finger protein, which we call ZFP47. ZFP47 interacts with DmCK2α but not with DmCK2β, indicating that this interaction is specific for the catalytic subunit of CK2. In situ hybridization to polytene chromosomes indicates that the corresponding gene is located at the 72A interval of chromosome III. Sequence analysis indicates that ZFP47 contains a consensus site for phosphorylation by CK2, 4 C2H2-type zinc-fingers, and a bipartite nuclear localization signal. Consistent with the prediction of a site for phosphorylation by CK2, we demonstrate that ZFP47 is phosphorylated by CK2 purified from Drosophila embryos. These studies demonstrate that ZFP47 is a new physiological partner and substrate of CK2. (Mol Cell Biochem 227: 99–105, 2001)

Key words

protein kinase CK2 Drosophila two hybrid 


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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Madhavi Kalive
    • 1
  • Regina L. Trott
    • 1
  • Ashok P. Bidwai
    • 1
    • 2
  1. 1.Department of BiologyUSA
  2. 2.Mary Babb Randolph Cancer CenterWest Virginia UniversityMorgantownUSA

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