Abstract
A reliable quantitative assay is necessary, when the possible biological role of PPi is studied. As presented below many different methods have been described, but the ideal one is still missing. In this chapter I shall describe with my comments all those methods that I have been able to find in the literature. However, before that I shall review the two, often combined phases, that precede the actual quantification: sampling and extraction. It is crucial to the successful assay that the PPi concentration is kept on the in vivo level in these processes.
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© 2001 Springer Science+Business Media New York
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Heinonen, J.K. (2001). Determination of PPi Concentration in Living Systems. In: Biological Role of Inorganic Pyrophosphate. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1433-6_2
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DOI: https://doi.org/10.1007/978-1-4615-1433-6_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5551-9
Online ISBN: 978-1-4615-1433-6
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