Models of Neuronal Bursting Behavior: Implications for In-Vivo Versus In-Vitro Respiratory Rhythmogenesis

  • Ilya A. Rybak
  • Walter M. St John
  • Julian F. R. Paton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 499)

Abstract

Neonatal in-vitro preparations can generate respiratory-like oscillations based on the intrinsic bursting or pacemaker properties in some neurons within the medullary preBötzinger complex (PBC)1-3. This discovery provided a basis for a hybrid pacemaker-network theory that suggests that pacemaker PBC neurons form a kernel of the central pattern generator and hence provide a necessary contribution to the genesis of the respiratory rhythm4. However, it is controversial whether a pacemaker-based mechanism necessarily operates in vivo. Specifically, the pacemaker-based models face principal problems in providing explanations to systems-level phenomena such as an independent regulation of respiratory phases, respiratory reflexes (e.g. the Hering-Breuer reflex), various phase resetting phenomena. On the other hand, the network theories and models based on in-vivo data can explain these phenomena5,6, but demand the respiratory phase transitions to be based on the reciprocal inhibitory interactions between respiratory neurons. Therefore, the network-based models have so far been unable to explain a pacemaker-driven rhythm recorded in vitro which is resistant to blockade of synaptic inhibition7. The current state of knowledge in the field requires a comprehensive computational study of the relationships between in-vivo and in-vitro data. The objective of this study was to explore the concept that the respiratory network can generate a breathing pattern by either a network or a hybrid pacemaker-network mechanism, which is state-dependent.

Keywords

Depression Glycine Respiration Washout Strychnine 

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Ilya A. Rybak
    • 1
  • Walter M. St John
    • 2
  • Julian F. R. Paton
    • 3
  1. 1.School of Biomedical EngineeringScience and Health Systems, Drexel UniversityPhiladelphiaPAUSA
  2. 2.Department of PhysiologyDartmouth Medical SchoolLebanonUSA
  3. 3.Department of PhysiologySchool of Medical Sciences, University of BristolUK

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