Detection of Four Human Milk Groups with Respect to Lewis-Bloodgroup-Dependent Oligosaccharides by Serologic and Chromatographic Analysis
Oligosaccharides from human milk samples obtained from individual donors were analyzed using high-pH anion-exchange chromatography. Three patterns of neutral oligosaccharides were detected corresponding to milk groups already described. These oligosaccharide groups correspond to the Lewis blood types Lea-b+Lea+b-and Lea-b-. A new carbohydrate pattern was detected in a milk sample from a Lea-b-person in which only nonfucosylated oligosaccharides and compounds bearing al,3-linked fucosyl residues were found. This finding led to the hypothesis that there exist 4 different oligosaccharide milk groups that fit well to the genetic basis of the Lewis blood group system.
KeywordsMilk Sample Human Milk Fucose Residue Milk Oligosaccharide Human Milk Sample
Unable to display preview. Download preview PDF.
- Bruntz R, Dabrowski U, Dabrowski J, Ebersold A, Peter-Katalinic J, Egge H. Fucose-containing oligosaccharides from human milk from a donor of blood group O Lea nonsecretor. Biol Chem HoppeSeyler 1988;369:257–273.Google Scholar
- Haeuw-Fievre S, Wieruszeski JM, Plancke Y, Michalski JC, Montreuil J, Strecker G. Primary structure of human milk octa-, dodeca-and tridecasaccharides determined by a combination of 1 H-NMR spectroscopy and fast-atom-bombardment mass spectrometry. Evidence for a new core structure, the para-lacto-N-octaose. Eur J Biochem 1993;215:361–371.PubMedCrossRefGoogle Scholar
- Mollison PL. Blood Transfusion in Clinical Medicine, 6th ed. Oxford: Blackwell, 1979.Google Scholar