Mass Spectrometric Investigations of Human Milk Oligosaccharides
Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDITOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retentiontime GPC fractions with masses up to 4000 Da, which has not been reported before.
KeywordsSugar Citrate Fractionation Lactose Oligosaccharide
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- Pfenninger A, Karas M, Finke B, Stahl B, Sawatzki G. MS Investigations of large-size neutral and acidic oligosaccharides from human milk. Proceedings of the 45`hASMS Conference on Mass Spectrometry and Allied Topics; Palm Springs CA; June 1997. p 1020.Google Scholar
- Pfenninger A, Karas M, Finke B, Stahl B, Sawatzki G. Structural analysis of underivatized human milk oligosaccharides by NanoESl/Ion Trap MS“. Proceedings of the 46thASMS Conference on Mass Spectrometry and Allied Topics; Orlando FL; June 1998.Google Scholar