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Mass Spectrometric Investigations of Human Milk Oligosaccharides

  • Anja Pfenninger
  • M. Karas
  • B. Finke
  • B. Stahl
  • G. Sawatzki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 501)

Abstract

Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDITOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retentiontime GPC fractions with masses up to 4000 Da, which has not been reported before.

Keywords

Sialic Acid Human Milk Dihydroxy Benzoic Acid Fucose Residue Mass Spectrometric Investigation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Anja Pfenninger
    • 1
  • M. Karas
    • 1
  • B. Finke
    • 2
  • B. Stahl
    • 2
  • G. Sawatzki
    • 3
  1. 1.Instrumental Analytical ChemistryUniversity of Frankfurt/MGermany
  2. 2.Numico Research GermanyGermany
  3. 3.SciNuTec GmbHGermany

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