Mass Spectrometric Investigations of Human Milk Oligosaccharides
Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDITOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retentiontime GPC fractions with masses up to 4000 Da, which has not been reported before.
KeywordsSialic Acid Human Milk Dihydroxy Benzoic Acid Fucose Residue Mass Spectrometric Investigation
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