A Role for Ectoapyrases and Extracellular ATP in Plant Growth and Development

  • Stanley J. Roux
  • Collin Thomas
  • Asha Rajagopal


For those studying signal transduction, familiarity with the enzyme apyrase is likely to be restricted to the context of its use for scavenging unwanted ATP from a solution. Apyrases hydrolyze both the γ and β phosphate on ATP and the terminal phosphate on ADP, and they do so very efficiently. Assays that require the removal of ATP from solutions typically utilize apyrase to achieve this goal.


Cystic Fibrosis Transmembrane Conductance Regulator Purinergic Receptor ABCI Gene Study Signal Transduction Medullary Collect Duct Cell 
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  1. Abraham, E., Prat, A., Gerweck, L., Seneveratne, T., Arceci, R., Kramer, R., Guidotti, G., and Cantiello, H., 1993, The multidrug resistance (mdrI) gene product functions as an ATP channel.Proc. Natl. Acad. Sci. USA 90: 312–316.PubMedCrossRefGoogle Scholar
  2. Azam, F., and Hodson, R., 1977, Dissolved ATP in the sea and its utilization by marine bacteria.Nature 267: 696–697.PubMedCrossRefGoogle Scholar
  3. Boyum, R., and Guidotti, G., 1997, Glucose dependent, cAMP- mediated ATP efflux fromSaccharomyces cerevisiae.Microbiology 1 43: 1901–1908.Google Scholar
  4. Chen, Y., and Roux, S., 1986, Characterization of nucleoside triphosphatase activity in isolated pea nuclei and its photoreversible regulation by light.Plant Physiol.81: 609–613.PubMedCrossRefGoogle Scholar
  5. Dudler, R., and Hertig, C., 1992, Structure of anmdr-like gene fromArabidopsis thaliana.J.Biol. Chem. 261: 5882–5888.Google Scholar
  6. Froldi, G., Gallo, U., Ragazzi, E., and Caparrotta, L., 1999, 6-Benzylaminopurine: A plant derived cytokinin inducing positive inotropism by P2-purinoceptors.Planta Medica 55: 245–249.CrossRefGoogle Scholar
  7. Hsieh, H., Tong, C., Thomas, C., and Roux, S., 1996, Light-modulated abundance of an mRNA encoding a calmodulin-regulated, chromatin-associated NTPase in pea.Plant Mol.Biol.30: 135–147.PubMedCrossRefGoogle Scholar
  8. Matin, A., and Matin, M., 1982, Cellular levels, excretion, and synthesis rates of cyclic AMP inEscherichia coli grown in continuous culture.J. Bacteriol 149: 801–807.PubMedGoogle Scholar
  9. Reisin, I., Prat, A., Abraham, E., Amara, J., Gregory, R., Ausiello, D., and Cantiello, H., 1994, The cystic fibrosis transmembrane conductance regulator is a dual ATP and chloride channel. J.Biol. Chem.269: 20584–20591.Google Scholar
  10. Schwiebert, E., 1999, ABC transporter-facilitated ATP conductive transport.Am. J. Physiol.276: Cl–8Google Scholar
  11. Sidler, M., Hassa, P., Hasan, S., Ringli, C., and Dudler, R., 1998, Involvement of an ABC transporter in developmental pathway regulating hypocotyl cell elongation in the light.Plant Cell 10: 1623–1636.PubMedGoogle Scholar
  12. Suko, Y., Kawahara, K., Fukuda ,Y., and Masuda, Y., 1997, Nuclear and cytosolic calcium signaling induced by extracellular ATP in rat kidney inner medullary collecting duct cells.Biochem.Biophys. Res. Commun.234: 224–229.CrossRefGoogle Scholar
  13. Thomas, C., Sun, Y., Naus, K., Lloyd, A., and Roux, S., 1999, Apyrase functions in plant phosphate nutrition and mobilizes phosphate from extracellular ATP.Plant Physiol.99: 543–551.CrossRefGoogle Scholar
  14. Todorov, L., Mihaylova-Todorova, S., Westfall, T., Sneddon, P., Kennedy, C., Bjur, R., and Westfall, D., 1997, Neuronal release of soluble nucleotidases and their role in neurotransmitter inactivation.Nature 387: 76–79.PubMedCrossRefGoogle Scholar
  15. Tong, C., Dauwalder, M., Clawson, G., Hatem, C., and Roux, S., 1993, The major nucleoside triphosphatase in Pea(Pisum sativum L.) nuclei and rat liver share common epitopes also present on nuclear lamins.Plant Physiol. 101: 1005–10011.PubMedCrossRefGoogle Scholar
  16. Urban M., Bhargava, T., and Hamer, J., 1999, An ATP-driven efflux pump is a novel pathogenicity factor in rice blast disease.EMBO J. 18: 512–521.PubMedCrossRefGoogle Scholar
  17. Wang, T-F., Ou, Y., and Guidotti, G., 1998, The transmembrane domains of ectoapyrase CD39) affect its enzymatic activity and quaternary structure.J. Biol. Chem.273: 24814–24821PubMedCrossRefGoogle Scholar
  18. Zheng, L., Zychlinsky, A., Liu, C, Ojcius, D., and Young, J., 1991, Extracellular ATP as a trigger for apoptosis or programmed cell death.J. Cell Biol. 112: 279–288.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Stanley J. Roux
    • 1
  • Collin Thomas
    • 1
  • Asha Rajagopal
    • 1
  1. 1.Institute for Cellular and Molecular BiologyUniv. of Texas at AustinAustinUSA

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