Plastid Ribosome Biogenesis During the Early Steps of Chloroplast Differentiation

Elements Controlling the Activation of Nuclear Genes Encoding Plastid Ribosomal Proteins
  • Regis Mache
  • Jean-Luc Gallois
  • Patrick Achard


Chloroplast differentiation is accomplished in several steps. In embryonic and meristematic cells, proplastids are present, characterised by their small size and the absence of internal membranes. They contain a low copy number of plastid DNA and few plastid ribosomes. Then, plastid differentiation follows several ways depending on plant organs or tissues. Because of its importance for photosynthesis, differentiation of proplastids into chloroplasts has been studied in details. It involves the co-ordinated expression of the nuclear genome and of the plastid genome. In the course of differentiation two main steps can be distinguished: firstly an early step during which the complexes necessary for plastid gene expression are synthesised and secondly, the formation of the photosynthetic apparatus. We will focus the present study on the expression of nuclear genes encoding plastid ribosomal proteins (r-proteins) that are important for early plastid differentiation.


Ribosomal Protein Nuclear Gene Ribosomal Protein Gene Plastid Gene Expression Chloroplast Differentiation 
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  1. Agafonov, D.E., Kolb, v.A., Nazimov, I.V., and Spirin, A.S., 1999, A protein residing at the interface of the bacterial ribosome. Proc. Natl. Acad. Sci. USA 96: 12345–12349.PubMedCrossRefGoogle Scholar
  2. Bisanz-Seyer, C., Li, Y-F., Seyer, P., and Mache, R, 1989, The components of the plastid ribosome are not accumulated synchronously during the early development of spinach plants. Plant. Mol. Biol. 12: 201–211.CrossRefGoogle Scholar
  3. Franzetti, B., Carol, P., and Mache, R, 1992a, Characterization and RNA-binding properties of a chloroplast Sl-like ribosomal protein. J. Biol Chem. 267: 19075–19081.PubMedGoogle Scholar
  4. Franzetti, B., Zhou, D.-X., and Mache, R, 1992b, Structure and expression of the nuclear gene coding for the plastid CS1 ribosomal protein from spinach. Nucl. Acids Res. 20: 4153–4157.PubMedCrossRefGoogle Scholar
  5. Harrak, H., Lagrange, T., Bisanz-Seyer, C., Lerbs-Mache, S., and Mache, R., 1995, The expression of nuclear genes encoding plastid ribosomal proteins precedes the expression of chloroplast genes during early phases of chloroplast development. Plant Physiol 108: 685–692.PubMedCrossRefGoogle Scholar
  6. Lagrange, T., Franzetti, B., Axelos, M., Mache, R., and Lerbs-Mache, S., 1993, Structure and expression of the nuclear gene coding for the chloroplast ribosomal protein L21: developmental regulation of a housekeeping gene by alternative promoters. Mol. Cell.Biol 13: 2614–2622.PubMedGoogle Scholar
  7. Lagrange, T., Gauvin, S., Hye-Jeong, Y., and Mache, R., 1997, S2F, a leaf-specific transacting factor, binds to a novel cis-acting element and differently activates the rpl21 gene. Plant Cell 9: 1469–1479.PubMedGoogle Scholar
  8. Li, Y-F., Zhou, D.-X., Clabault, G., Bisanz-Seyer, C., and Mache, R., 1995, Cis-acting elements and expression pattern of the spinach rps22 gene coding for a plastid-specific ribosomal protein. Plant Mol. Biol. 28: 595–604.PubMedCrossRefGoogle Scholar
  9. Martin, W., Lagrange, T., Li, Y -F., Bisanz-Seyer, C., and Mache, R., 1990, Hypothesis for the evolutionary origin of the chloroplast ribosomal protein L21 of spinach. Curr. Genet 18: 553–556.PubMedCrossRefGoogle Scholar
  10. Trifa, Y., Privat, I., Gagnon, J., Baeza, L., and Lerbs-Mache, S., 1998, The nuclear RPL4 gene encodes a chloroplast protein that co-purifies with the T7-like transcription complex as weIl as plastid ribosomes. J. Biol Chem. 273: 3980–3985.PubMedCrossRefGoogle Scholar
  11. Villain, P., Mache, R., and Zhou, D-X., 1996, GT element-mediated cell type-specific transcriptional control. J. Biol Chem. 271: 32593–32598.PubMedCrossRefGoogle Scholar
  12. Zhou, D.-X., Li, Y.-F., Rocipon, M., and Mache, R., 1992, Sequence-specific interaction between SIF, a spinach nuclear factor, and a negative cis-element conserved in proplastid-related genes. J. Biol Chem. 267: 23515–23519.PubMedGoogle Scholar
  13. Zhou, D.-X. and Mache, R., 1989, Presence in the stroma of chloroplasts of a large pool of a ribosomal protein not structurally related to any Escherichia coli protein. Mol. Gen.Genet. 219: 204–208.PubMedCrossRefGoogle Scholar
  14. Zhou, D.-X. and Mache, R., 1989, Presence in the stroma of chloroplasts of a large pool of a ribosomal protein not structurally related to any Escherichia coli protein. Erratum, Mol. Gen. Genet 1990, 223: 167.PubMedCrossRefGoogle Scholar
  15. Zhou, D.-X., 1999, Regulatory mechanism of plant gene transcription by GT-elements and GT-factors. Trends Plant Science 4: 210–214.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Regis Mache
    • 1
  • Jean-Luc Gallois
    • 1
  • Patrick Achard
    • 1
  1. 1.Laboratoire de Génétique Moléculaire des Plantes, UMR 5575. CNRSUniversité J. FourierGrenoble cedex 9France

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