Infectious Bronchitis Virus Envelope Protein Targeting: Implications for Virus Assembly

  • Emily Corse
  • Carolyn E. Machamer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

Coronaviruses acquire their membrane envelope by budding into the lumen of Golgi and pre-Golgi compartments. After budding, virions are thought to move in vesicles through the secretory pathway, and exit the cell when these vesicles fuse with the plasma membrane (Holmes et al., 1981, Tooze et al., 1987). The specific compartment into which coronaviruses bud is the cis-Golgi network (CGN), also known as the endoplasmic reticulum Golgi intermediate compartment (Krijnse Locker et al., 1994). Just as enveloped viruses that bud from the plasma membrane must direct the accumulation of their envelope proteins at the cell surface, coronaviruses must localize their envelope proteins to the membranes of the CGN.

Keywords

Hepatitis Microwave Bronchitis Vaccinia Carol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baudoux, P., Carrat, C., Besnardeau, L., Charley, B., and Laude, H., 1998, Coronavirus pseudoparticles formed with recombinant M and E proteins induce alpha-interferon synthesis by leukocytes. J. Virol. 72:8636–8643.PubMedGoogle Scholar
  2. Brown, W. J., and Farquhar, M. G., 1989, Immunoperoxidase methods for the localization of antigens in cultured cells and tissue sections by electron microscopy. Methods Cell Biol. 31:553–569.PubMedCrossRefGoogle Scholar
  3. Cluett, E. B., and Machamer, C. E., 1996, The envelope of vaccinia virus reveals an unusual phospholipid in Golgi complex membranes. J. Cell Sci. 109:2121–2131.PubMedGoogle Scholar
  4. Corse, E., and Machamer, C. E., 2000, Infectious bronchitis virus E protein is targeted to the Golgi complex and directs release of virus-like particles. J. Virol. 74:4319–4326.PubMedCrossRefGoogle Scholar
  5. Fischer, F., Stegen, C. F., Masters, P. S., and Samsonoff, W. A., 1998, Analysis of constructed E gene mutants of mouse hepatitis virus confirms a pivotal role for E protein in Coronavirus assembly. J. Virol. 72:7885–7894.PubMedGoogle Scholar
  6. Godeke, G.-J., de Haan, C. A. M., Rossen, J. W. A., Vennema, H., and Rottier, P. J. M., 2000, Assembly of spikes into coronaviruses particles is mediated by the carboxy-terminal domain of the spike protein. J. Virol. 74:1566–1571.PubMedCrossRefGoogle Scholar
  7. Holmes, K. V., Doller, E. W., and Sturman, L. S., 1981, Tunicamycin resistant glycosylation of a Coronavirus glycoprotein: demonstration of a novel type of viral glycoprotein. Virol. 115:334–344.CrossRefGoogle Scholar
  8. Krijnse Locker, J., Ericsson, M., Rottier, P. J.M, and Griffiths, G., 1994, Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular transport step. J. Cell Biol. 124:55–70.CrossRefGoogle Scholar
  9. Liu, D. X., Cavanagh, D., Green, P., and Inglis, S. C., 1991, A polycistronic mRNA specified by the Coronavirus infectious bronchitis virus. Virol. 184:531–544.CrossRefGoogle Scholar
  10. Machamer, C. E., Mentone, S. A., Rose, J. K., and Farquhar, M. G., 1990, The El glycoprotein of an avian Coronavirus is targeted to the cis Golgi complex. Proc. Natl. Acad. Sci. USA. 87:6944–6948.PubMedCrossRefGoogle Scholar
  11. Tooze, J., Tooze, S. A., and Fuller, S. D., 1987, Sorting of progeny Coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells. J. Cell Biol. 105:1215–1226.PubMedCrossRefGoogle Scholar
  12. Vennema, H., Heijnen, L., Zijderveld, A., Horzinek, M. C., and Spaan, W. J. M., 1990, Intracellular transport of recombinant Coronavirus spike proteins: implications for viral assembly. J. Virol. 64:339–346.PubMedGoogle Scholar
  13. Vennema, H., Godeke, G.-J., Rossen, J. W. A., Voorhout, W. F., Horzinek, M. C., Opstelten, D.-J. E., and Rottier, P. J. M., 1996, Nucleocapsid-independent assembly of coronavirus-like particles by co-expression of viral envelope protein genes. EMBO J. 15:2020–2028.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Emily Corse
    • 1
  • Carolyn E. Machamer
    • 1
  1. 1.Department of Cell Biology and AnatomyThe Johns Hopkins University School of MedicineBaltimoreUSA

Personalised recommendations