Expression of Transcriptional Units Using Transmissible Gastroenteritis Coronavirus Derived Minigenomes and Full-length cDNA Clones

  • Isabel Sola
  • Sara Alonso
  • Carlos Sanchez
  • J. Manuel Sanchez-Morgado
  • Luis Enjuanes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

Many factors including RNA primary and secondary structure and protein-RNA interactions may regulate mRNA abundance. The nature of the transcription regulatory sequences (TRSs) and the extent of their complementarity to the leader 3’ end may be the most relevant and is discussed below. The TRSs include the core sequence (CS), previously named intergenic sequence (IG), that is a short conserved sequence element upstream the transcription units, and flanking sequences located upstream and downstream the CS. A helper dependent expression system based on transmissible gastroenteritis Coronavirus (TGEV) derived minigenomes, encoding new subgenomic mRNAs, has been used to study the elements that regulate transcription in Coronavirus. The optimization of TRSs can lead to the improvement of mRNA levels using both minigenomes and full-length cDNA clones.

Keywords

Hepatitis Recombination Gastroenteritis 

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References

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Isabel Sola
    • 1
  • Sara Alonso
    • 1
  • Carlos Sanchez
    • 1
  • J. Manuel Sanchez-Morgado
    • 1
  • Luis Enjuanes
    • 1
  1. 1.Department of Molecular and Cell Biology, Centro Nacional de BiotecnologíaCSIC Campus Universidad AutónomaMadridSpain

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