Expression of Transcriptional Units Using Transmissible Gastroenteritis Coronavirus Derived Minigenomes and Full-length cDNA Clones
Many factors including RNA primary and secondary structure and protein-RNA interactions may regulate mRNA abundance. The nature of the transcription regulatory sequences (TRSs) and the extent of their complementarity to the leader 3’ end may be the most relevant and is discussed below. The TRSs include the core sequence (CS), previously named intergenic sequence (IG), that is a short conserved sequence element upstream the transcription units, and flanking sequences located upstream and downstream the CS. A helper dependent expression system based on transmissible gastroenteritis Coronavirus (TGEV) derived minigenomes, encoding new subgenomic mRNAs, has been used to study the elements that regulate transcription in Coronavirus. The optimization of TRSs can lead to the improvement of mRNA levels using both minigenomes and full-length cDNA clones.
KeywordsGreen Fluorescent Protein Bacterial Artificial Chromosome Flank Sequence Core Sequence Heterologous Gene
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