Abstract
Demyelination is the hallmark pathologic process of several viral-induced, and inflammatory, immune-mediated diseases of the central nervous system (Allen and Bradkin 1993). Several experimental animal model systems are used to study demyelination, including MHV-induced demyelination in mice (Stohlman et al 1981; Wege et al 1982; Lavi et al 1984; Lavi et al 1984b; Lavi and Weiss 1989; Houtman et al 1996; Lavi et al 1999). Previous studies with this model suggested that the S protein and gene may be important for pathogenesis since the spike (S) protein participates in many functions related to virus-host interactions (Dalziel et al 1986). Thus we wanted to explore the possibility that the S gene contains molecular determinants of demyelination. We used targeted RNA recombination as previously described (Peng et al 1995; Leparc-Goffart et al 1998; Phillips et al 1999) to insert the S gene of a non-demyelinating virus (MHV-2) into the background of a demyelinating virus (MHV-A59). We then studied the pathogenesis of the new recombinant viruses.
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Sarma, J.D., Fu, L., Weiss, S.R., Lavi, E. (2001). Demyelination Determinants in the S Gene of MHV. In: Lavi, E., Weiss, S.R., Hingley, S.T. (eds) The Nidoviruses. Advances in Experimental Medicine and Biology, vol 494. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1325-4_21
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DOI: https://doi.org/10.1007/978-1-4615-1325-4_21
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