Mutation of the Immunodominant CD8+ Epitope in the MHV-4 Spike Protein
Our lab focuses on understanding the mechanism of murine Coronavirus mouse hepatitis virus (MHV) pathogenesis in the central nervous system (CNS). MHV strains MHV-4 (JHM) and A59 induce acute encephalitis, followed by chronic demyelination in weanling C57B1/6 (B6) mice; virus is cleared from the CNS by about 2 weeks post infection and demyelination occurs in the absence of infectious virus. We have previously used targeted recombination to select isogenic viruses differing only in the spike gene, expressing either the A59 spike (SA59R16) or the MHV-4 spike (S4R29) in the background of the A59 genome (Phillips et al., 1999). While the MHV-4 spike expressing virus (S4R29) is, like MHV-4, highly neurovirulent, the SA59R16, like MHV-A59, is significantly less neurovirulent
KeywordsHepatitis Chromium Recombination Encephalitis Asparagine
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