Effects of TaClo (1-Trichloromethyl-1,2,3,4-Tetrahydro-ß-Carboline) on Mice Neuronal Culture and Possibilities of Cellular Regeneration in Vitro after Toxin Withdrawal

Abstract

TaClo (l-trichloromethyl-1,2, 3,4-tetrahydro-b-carboline), a highly chlorinated tetrahydro-ß-carboline is known to exert neurotoxic effects an the dopaminergic system both in vivo and in vitro. Toxicity may derive from endogenous formation of addition products of exogenous chloral sources with endogenously present tryptamine chemical. TaClo’s chemical structure is analogous to the dopaminergic neurotoxin MPTP (N-methyl-4-phenyl-l,2,3,6-tetrahydropyridine) and was shown to be a potent toxin that induces neurodegenerative processes thus offering a good model system in cell culture. The aim of the present investigation is to study the effect of TaClo an OF1/SPF mice neuronal culture and to observe the regenerative properties of differentiated dopaminergic neurones in vitro after toxin (50 µM TaClo) withdrawal.