The Possible Role of Intestinal Surfactantlike Particles in the Absorption of Triacylglycerols in the Rat

  • David H. Alpers
  • Michael J. Engle
  • Rami Eliakim


The process of triacylglycerol absorption has passed through at least three quantum leaps of progress. In the 1950s and 1960s there was a tremendous increase in knowledge concerning the intralumenal and intracellular fate of ingested triacylglycerols. By the end of this explosion of information the current concepts were defined: intralumenal hydrolysis, incorporation of fatty acids and monoglycerides into mixed bile salt micelles, uptake of lipid (not by pinocytosis) into the enterocyte with reformation of triacylglycerols, and finally, intracellular packaging and secretion into lymph via lipoproteins (Senior, 1964; Hofmann and Small, 1967; Johnston, 1968; Borgström, 1974; Friedman and Nylund, 1980). In addition, the intracellular pathways of fat absorption were described using transmission electron microscopy (Palay and Karlin, 1959; Strauss, 1966; Rubin, 1966; Sjostrand and Borgstrom, 1967). Although the details and mechanisms of the lumenal events were largely understood at that time, the precise intracellular events and their compartmentalization were unclear.


Lamina Propria Microsomal Triglyceride Transfer Protein Intestinal Alkaline Phosphatase Apical Compartment Triacylglycerol Absorption 
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  1. Alpers, D. H., Zhang, Y., and Ahnen, D. J., 1995, Synthesis and parallel secretion of rat intestinal alkaline phosphatase and a surfactant-like particle protein,Am. J. Physiol.268 (Endocrinol. Metab. 31):E1205–E1214.PubMedGoogle Scholar
  2. Bayer, P. M., and Pointner, H., 1980, The action of somatostatin on intestinal alkaline phosphatase stimulated by secretin and cholecystokinin-pancreozymin,Clin. Chim. Acta 108:129–134.PubMedCrossRefGoogle Scholar
  3. Beil, F.W., and Grundy, S. M, 1980, Studies on plasma lipoproteins during absorption of exogenous lecithin in man,J. Lipid Res.21:525–536.PubMedGoogle Scholar
  4. Borgström B., 1974, Fat digestion and absorption, in:Biomembranes Volume 4B (D. H. Smyth, ed.), Plenum Press, New York, pp. 555–620.Google Scholar
  5. Cardwell, R. R., Jr., Badenhausen, S., and Porter, K. R., 1967, Intestinal triglyceride absorption in the rat. An electron microscopic study,J. Cell Biol.34:123–155.CrossRefGoogle Scholar
  6. Chu, S. W., Geyer, R. P., and Walker, W. A., 1987, Myo-inositol action on gerbil intestine: Alterations in alkaline phosphatase activity upon phosphatidylinositol depletion and repletionin vivo, Biochim. Biophys. Acta 929:220–225.PubMedCrossRefGoogle Scholar
  7. Day, A. P., Fheher, M. D., Chopra, R., and Mayne, P. D., 1991, Triglyceride fatty acid chain length influences the postprandial rise in serum intestinal alkaline phosphatase activity,Ann. Clin. Biochem.29:287–291.Google Scholar
  8. DeSchryver-Kecskemeti, K., Eliakim, R., Carroll, S., Stenson, W. F., Moxley, M. A., and Alpers, D. H., 1990, Intestinal surfactant-like material: A novel secretory product of the rat enterocyte,J. Clin. Invest 84:1355–1361.CrossRefGoogle Scholar
  9. DeSchryver-Kecskemeti, K., Eliakim, R., Green, K., and Alpers, D. H., 1991, A novel intracellular pathway for rat intestinal digestive enzymes (alkaline phosphatase and sucrase) via a lamellar particle,Lab. Invest.65:365–373.PubMedGoogle Scholar
  10. Domar, U., Karpe, F., Hamsten, A., Stigbrand, T., and Olivecrona, T., 1993, Human intestinal alkaline phosphatase-Release to the blood is linked to lipid absorption, but removal from the blood is not linked to lipoprotein clearance,Eur. J. Clin. Invest.23:753–760.PubMedCrossRefGoogle Scholar
  11. Eliakim, R., DeSchryver-Kecskemeti, K., Nogee, L., Stenson, W. F., and Alpers, D. H., 1989, Isolation and characterization of a small intestinal surfactant-like particle containing alkaline phosphatase and other digestive enzymes,J. Biol. Chem.264:20614–20619.PubMedGoogle Scholar
  12. Eliakim, R., Becich, M. J., Green, K., and Alpers, D. H., 1990, Both tissue and serum phospholipases release rat intestinal alkaline phosphatase,Am. J. Physiol.259 (Gastrointest. Liver Physiol. 22):G618–G625.PubMedGoogle Scholar
  13. Eliakim, R., Mahmood, A., and Alpers, D. H., 1991a. Rat intestinal alkaline phosphatase secretion into lumen and serum is coordinately regulated,Biochim. Biophys. Acta 1091:1–8.PubMedCrossRefGoogle Scholar
  14. Eliakim, R., Becich, M. J., Green, K., and Alpers, D. H., 1991b, The developmental expression of intestinal surfactant-like particles in the rat,Am. J. Physiol.261(Gastrointest. Liver Physiol. 24):G269–G279.PubMedGoogle Scholar
  15. Eliakim, R., Goetz, G. S., Rubio, S., Chaille-Heu, B., Shao, J.-S., Ducroc, R., and Alpers, D. H., 1997, Isolation and characterization of surfactant-like particles in rat and human colon,Am. J. Physiol.272(Gastrointest. Liver Physiol. 35):G425–G434.PubMedGoogle Scholar
  16. Engle, M. J., and Alpers, D. H., 1992, The two mRNAs encoding rat intestinal alkaline phosphatase represent two unique nucleotide sequences,Clin. Chem.38:2506–2509.PubMedGoogle Scholar
  17. Engle, M. J., Grove, M. L., Becich, M. J., and Alpers, D. H., 1995, Appearance of surfactant-like particles in apical medium of Caco-2 cells may occur via tight junctions,Am. J. Physiol 268(Cell Physiol. 37):C1401–C1413.PubMedGoogle Scholar
  18. Friedman, H. I., and Nylund, B., 1980. Intestinal fat digestion, absorption, and transport: A review,Am. J. Clin. Nutr.33:1108–1139.PubMedGoogle Scholar
  19. Glickman, R. M., Alpers, D. H., Drummey, G. D., and Isselbacher, K. J., 1970, Increased lymph alkaline phos-phatase after fat feeding; Effects of medium chain triglycerides and inhibition of protein synthesis,Biochim. Biophys. Acta 201:226–235.PubMedCrossRefGoogle Scholar
  20. Goetz, G. S., Shao, J.-S., and Alpers, D. H., 1997. Purification and initial characterization of a surfactant-like particle (SLP) from human stomach,Gastroenterol.112:A128.Google Scholar
  21. Halbhuber, K. J., Schulze, M., Rhode, H., Bublitz, R., Feuerstein, H., Walter, M., Linss, W., Meyer, H. W., and Horn, A., 1994. Is the brush border membrane of the intestinal mucosa a generator of “chymosomes”?Cell Molec. Biol.40:1077–1096.Google Scholar
  22. Havel, R. J., 1980, Lipoprotein biosynthesis and metabolism, in:Lipoprotein Structure, (A. M. Scanu and F. R. Landsberger, eds.), New York Academy of Science, New York, pp. 16–29.Google Scholar
  23. Hills, B. A., Butler, B. D., and Lichtenberger, L. M., 1983, Gastric mucosal barrier: Hydrophobic lining to the lumen of the stomach,Am. J. Physiol.244(Gastrointest. Liver Physiol. 7):G561–G568.PubMedGoogle Scholar
  24. Hofmann, A. F., and Small, D. S., 1967, Detergent properties of bile salts: Correlation with physiological function,Ann. Rev. Med.18:333–376.PubMedCrossRefGoogle Scholar
  25. Johnston, J. M, 1968, Mechanism of fat absorption, in:Handbook of Physiology, Volume 3 (J. Field, ed.), American Physiological Society, Washington, D.C., pp. 1353–1375.Google Scholar
  26. Kao, Y. C., and Lichtenberger, L. M., 1987, Localization of phospholipid-rich zones in rat gastric mucosa: Possible origin of a protective hydrophobic lumenal lining,J. Histochem. Cytochem.35:1285–1298.PubMedCrossRefGoogle Scholar
  27. Kleerekoper, M., Home, M., Cornish, C. J., and Posen, S., 1970, Serum alkaline phosphatase after fat ingestion: An immunological study,Clin. Sci.38:339–345.PubMedGoogle Scholar
  28. Kumar, N. S., and Mansbach, C. M., 1997, Determinants of triacylglycerol transport from the endoplasmic retic-ulum to the Golgi in the intestine,Am. J. Physiol.273(Gastrointest. Liver Physiol. 36):G18–G30.PubMedGoogle Scholar
  29. Lam, K. C, and Mistilis, S. P., 1973, Role of intestinal alkaline phosphatase in fat transport,Aust. J. Exp. Biol. Med. Sci.51:411–416.PubMedCrossRefGoogle Scholar
  30. Langman, M. J. S., Leuthold, E., Robson, E. B., Luffman, J. E., and Harris H., 1966, Influence of diet on the “intestinal” component of serum alkaline phosphatase in people of differerent ABO blood groups and secretor status,Nature 212:41–43.PubMedCrossRefGoogle Scholar
  31. Lin, M. C., Arbeeny, C., Bergquist, K., Kienzle, B., Gordon, D. A., and Wetterau, J. R., 1994, Cloning and regulation of hamster microsomal triglyceride transfer protein. The regulation is independent from that of other hepatic and intestinal proteins which participate in the transport of fatty acids and triglycerides,J. Biol. Chem.269:29138–29145.PubMedGoogle Scholar
  32. Mahley, R. W, Bennett, B. D., Morre, D. J., Gray, M. E., Thistlethwaite, W., and LeQuire, V. S., 1971, Lipoproteins associated with Golgi apparatus isolated from epithelial cells of rat small intestine,Lab. Invest.25:435–444.PubMedGoogle Scholar
  33. Mahmood, A., Mahmood, S., DeSchryver-Kecskemeti, K., and Alpers, D. H., 1993, Characterization of proteins in rat and human intestinal surfactant-like particles,Arch. Biochem. Biophys.300:280–286.PubMedCrossRefGoogle Scholar
  34. Mahmood, A., Yamagishi, F, Eliakim, R., DeSchryver-Kecskemeti, K., Gramlich, T. L., and Alpers, D. H., 1994, A possible role for rat intestinal surfactant-like particles in transepithelial triacylglycerol transport,J. Clin. Invest.93:70–80.PubMedCrossRefGoogle Scholar
  35. Malagelada, J. R., Stolbach, L. L., and Linscheer, W. G., 1977, Influence of carbon chain length of dietary fat on intestinal alkaline phosphatase in chylous ascites,Am. J. Dig. Dis.22:629–632.PubMedCrossRefGoogle Scholar
  36. Mansbach, C. M., and Nevin, P., 1994, Effect of brefeldin A on lymphatic triacylglycerol transport in the rat,Am. J. Physiol.266 (Gastrointest. Liver Physiol. 29):G292–G302.PubMedGoogle Scholar
  37. Miura, S. M., Asakura, H., Miyairi, M., Morishita, T., Ishii, H., and Tsuchiya, M., 1979, Study on the fat absorption and transportation into intestinal lymph of rats. Differences in the absorption of saturated and unsaturated long chain fatty acids and the role of intestinal alkaline phosphatase,JPN J. Gastroenterol.76:871–880.Google Scholar
  38. Miura, S., Asakura, H., Miyairi, M., Morishita, T., Nagata, H., and Tsuchiya, M., 1982, Effect of colchicine on intestinal alkaline phosphatase activity during linoleic acid absorption in rats,Digestion 23:224–231.PubMedCrossRefGoogle Scholar
  39. Palay, S. L., and Karlin, L. J., 1959, An electron microscopic study of the intestinal villus. II. The pathway of fat absorption,J. Biophys. Biochem. Cytol.5:373–384.PubMedCrossRefGoogle Scholar
  40. Parlier, R. D., Frase, S., and Mansbach, C. M. II., 1989, Intraenterocyte distribution of absorbed lipid and effects of phosphatidylcholine,Am. J. Physiol 256 (Gastrointest. Liver Physiol. 19):G349–G355.PubMedGoogle Scholar
  41. Redgrave, T. G., 1971, Association of Golgi membranes with lipid droplets (prechylomicrons) from within intestinal epithelial cells during absorption of fat,Aust. J. Exp. Biol. Med. Sci.49:209–224.PubMedCrossRefGoogle Scholar
  42. Rubin, C. E., 1966, Electron microscopic studies of triglyceride absorption in man,Gastroenterol 50:65–77.Google Scholar
  43. Rufo, M. B., Malagelada, J. R., Linscheer, W. G., and Fishman, W. H., 1973, Metabolic variants of intestinal alkaline phosphatase in relation to fat absorption: In situ demonstration with the organ-specific inhibitors l-phenylalanine and l-homoarginine,Histochemie 33:313–322.PubMedCrossRefGoogle Scholar
  44. Senior, J. R., 1964, Intestinal absorption of fat,J. Lipid Res.5:495–521.PubMedGoogle Scholar
  45. Sjostrand, F. S., and Borgström, B., 1967, The lipid components of the smooth-surfaced membrane-bounded vesicles of the columnar cells of the rat intestinal epithelium during fat absorption,J. Ultrastruct. Res.20:140–160.PubMedCrossRefGoogle Scholar
  46. Strauss, E. W., 1966, Electron microscopic study of intestinal fat absorption in vitro from mixed micelles containing linolenic acid, monoolein and bile salt,J. Lipid Res.7:307–323.PubMedGoogle Scholar
  47. Sussman, N. L., Eliakim, R., Rubin, D., Perlmutter, D. H., DeSchryver-Kecskemeti, K., and Alpers, D. H., 1989, Intestinal alkaline phosphatase is secreted bidirectionally from villous enterocytes,Am. J. Physiol.257 (Gastrointest. Liver Physiol. 20):G14–G23.PubMedGoogle Scholar
  48. Tietze, C. C., Becich, M. J., Engle, M., Stenson, W. F., Eliakim, R., and Alpers, D. H., 1992, Caco-2 cell trans-fection by rat intestinal alkaline phosphatase cDNA increases surfactant-like particles,Am. J. Physiol.263 (Gastrointest. Liver Physiol. 26):G756–G766.PubMedGoogle Scholar
  49. Tso, P., Drake, D. S., Black, D. D., and Sabesin, S. M., 1984, Evidence for separate pathways of chylomicron and very low-density lipoprotein assembly and transport by rat small intestine,Am. J. Physiol.247 (Gastrointest. Liver Physiol. 10):G599–G610.PubMedGoogle Scholar
  50. Yamagishi, F., Komoda, T., and Alpers, D. H., 1994a. Secretion and distribution of rat intestinal surfactant-like particles following fat feeding,Am. J. Physiol.266 (Gastrointest. Liver Physiol. 29):G944–G952.PubMedGoogle Scholar
  51. Yamagishi, E., Becich, M. J., Evans, B. A., Komoda, T., and Alpers, D. H., 1994b, The clearance of surfactant-like particle (SLP) proteins from the circulation in rats,Am. J. Physiol.266 (Gastrointest. Liver Physiol. 29):G596–G605.PubMedGoogle Scholar
  52. Young, G. P., Friedman, S., Yedlin, S. T., and Alpers, D. H., 1981, Effect of fat-feeding on intestinal alkaline phosphatase activity in tissue and serum,Am. J. Physiol.241 (Gastrointest. Liver Physiol. 4):G461–G468.PubMedGoogle Scholar
  53. Wang, W., Wang, P., and Chaudry, I. H., 1997. Intestinal alkaline phosphatase: Role in the depressed gut lipid transport after trauma-hemorrhagic shock,Shock 8:40–44.PubMedCrossRefGoogle Scholar
  54. Wu, A. L., Clark, S. B., and Holt, P. R., 1975, Transmucosal triglyceride transport rates in proximal and distal rat intestinein vivo, J. Lipid Res.16:251–257.PubMedGoogle Scholar
  55. Zhang, Y., Shao, J.-S., Xie, Q., and Alpers, D. H., 1996, Immunolocalization of intestinal alkaline phosphatase and surfactant-like particle proteins in rat duodenum,Gastroenterol.110:478–488.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • David H. Alpers
    • 1
  • Michael J. Engle
    • 1
  • Rami Eliakim
    • 2
  1. 1.Division of Gastroenterology, Department of MedicineWashington University School of MedicineSt. Louis
  2. 2.Hadassah Medical SchoolJerusalemIsrael

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