Abstract
Free radicals and other paramagnetic species, play an important role in cellular injury and pathophysiology. EPR spectroscopy and imaging has emerged as an important tool for non-invasivein vivomeasurement and spatial mapping of free radicals in biological tissues. Extensive applications have been performed in small animals such as mice and recently applications in humans have been performed. Spatial EPR imaging enables 3D mapping of the distribution of a given free radical while spectral-spatial EPR imaging enables mapping of the spectral information at each spatial position, and, from the observed line width, the localized tissue oxygenation can be determined. A variety of spatial, and spectral-spatial EPR imaging applications have been performed. These techniques, along with the use of biocompatible paramagnetic probes including particulate suspensions and soluble nitroxide radicals, enable spatial imaging of the redox state and oxygenation in a variety of biomedical applications. With spectral-spatial EPR imaging, oxygenation was mapped within the gastrointestinal (GI) tract of living mice, enabling measurement of the oxygen gradient from the proximal to the distal GI tract. Using spatial EPR imaging, the distribution and metabolism of nitroxide radicals within the major organs of the body of living mice was visualized and anatomically co-registered by proton MRI enablingin vivomapping of the redox state and radical clearance. EPR imaging techniques have also been applied to non-invasively measure the distribution and metabolism of topically applied nitroxide redox probes in humans, providing information regarding the penetration of the label through the skin and measurement of its redox clearance. Thus, EPR spectroscopy and imaging has provided important information in a variety of applications ranging from small animal models of disease to topical measurement of redox state in humans. (Mol Cell Biochem 234/235: 359-367, 2002)
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References
Josephson RA, Silverman HS, Lakatta EG, Stem MD, Zweier JL: Study of the mechanisms of hydrogen-peroxide and hydroxyl free radical-induced cellular injury and calcium overload in cardiac myocytes. J Biol Chem 266: 2354–2361, 1991
Serra JA, Dominguez RO, de Lustig ES, Guareshi EM, Famulari AL, Bartolome EL, Marschoff ER: Parkinson’s disease is associated with oxidative stress: Comparison of peripheral antioxidant profiles in living Parkinson’s, Alzheimer’s and vascular dementia patients. J Neural Trans 108: 1135–1148, 2001
Winterbourn CC: Free-radical toxicology and antioxidant defense. Clin Exp Pharmacol Physiol 22: 877–880, 1995
Berliner LJ, Fujii H: Magnetic resonance imaging of biological specimens by electron paramagnetic resonance of nitroxide spin labels. Science 227: 517–519, 1985
Zweier JL, Kuppusamy P: Electron paramagnetic resonance measurements of free radicals in the intact beating heart: A technique for detection and characterization of free radicals in whole biological tissues. Proc Natl Acad Sci USA 85: 5703–5707, 1988
Halpern HJ, Spencer DP, Polen JV, Bowman, Nelson AC, Dowey EM, Teicher BA: Imaging radio frequency electron spin resonance spectrometer with high resolution and sensitivity forin vivomeasurements. Rev Sci Instrum 60: 1040–1050, 1989
Chen K, Ng C, Zweier JL, Glickson JD, Swartz HM: Measurement of the intracellular concentration of oxygen in a cell perfusion system. Magn Reson Med 31: 668–672, 1994
Kuppusamy P, Zweier JL: Evaluation of nitroxides for the study of myocardial metabolism and oxygenation. Magn Reson Chem 33: 5123–5128, 1995
Kuppusamy P, Chzhan M, Vij K, Shteynbuk M, Gianella E, Lefer DJ, Zweier JL: 3-Dimensional spectral spatial EPR imaging of free radicals in the heart. A technique for imaging tissue metabolism and oxygenation. Proc Natl Acad Sci USA 91: 3388–3392, 1994
Kuppusamy P, Chzhan M, Zweier JL: Development and optimization of three dimensional spatial EPR imaging for biological organs and tissues. J Magn Reson B106: 122–130, 1995
Kuppusamy P, Wang P, Zweier JL: Three-dimensional spatial EPR imaging of the rat heart. Magn Reson Med 34: 99–105, 1995
Kuppusamy P, Shankar RA, Zweier JL:In vivomeasurement of arterial and venous oxygenation in the rat using 3D spectral-spatial electron paramagnetic resonance imaging. Phys Med Biol 43: 1837–1844, 1998
Fuchs J, Groth NK, Herrling TE, Zimmer G: Electron paramagnetic resonance studies on nitroxide radical 2,2,5,5-tetramethyl-4-piperidin1-oxyl (TEMPO) redox reactions in human skin. Free Rad Biol Med 22: 967–976, 1997
Swartz HM, Clarkson RB: The measurement of oxygenin vivousing EPR techniques. Phys Med Biol 43: 1957–1975, 1998
Chzhan M, Kuppusamy P, Samouilov A, He G, Zweier JL: A tunable reentrant resonator with transverse orientation of electric field forin vivoEPR spectroscopy. J Magn Reson 137: 373–378, 1999
He G, Petryakov S, Samouilov A, Chzhan M, Kuppusamy P, Zweier JL: Development of a resonator with automatic tuning and coupling capability to minimize sample motion noise forin vivoEPR spectroscopy. J Magn Reson 149: 218–227, 2001
Petryakov S, Chzhan M, Samouilov A, He G, Kuppusamy P, Zweier JL: A bridged loop-gap S-band surface resonator for topical EPR spectroscopy. J Magn Reson 151: 124–128, 2001
Kuppusumy P, Chzhan M, Vij K, Shteynbuk M, Gianella E, Lefer DJ, Zweier JL: 3-Dimensional spectral spatial EPR imaging of free-radicals in the heart: A technique for imaging tissue metabolism and oxygenation. Proc Nail Acad Sci USA 91: 3388–3392, 1994
Kuppusamy P, Chzhan M, Samouilov A, Wang P, Zweier JL: Development and optimization of 3-dimensional spatial EPR imaging for biological organs and tissues. J Magn Reson Series B 107: 116–125, 1995
Kuppusamy P, Wang P, Shankar RA, Ma L, Trimble CE, Hsia CJC, Zweier JL:In vivotopical EPR spectroscopy and imaging of nitroxide free radicals and polynitroxyl-albumin. Magn Reson Med 40: 806–811, 1998
Zweier JL, Li H, Deng Y, Kuppusamy P, Lurie D: Application of PEDRI to measure the invivodistribution and clearance of a triaryl methyl radical in mice. Proc Intl Soc Magn Reson Med: 938, 2001
Sheridan WG, Lowndes RH, Young HL: Intraoperative tissue oximetry in the human gastrointestinal-tract. Am J Surg 159: 314–319, 1990
Hauser CJ, Locke RR, Kao HW, Patterson J, Zipser RD: Visceral surface oxygen-tension in experimental colitis in the rabbit. J Lab Clin Med 112: 68–71, 1988
Cooper GJ, Sherry KM, Thorpe JA: Changes in gastric tissue oxygenation during mobilization for esophageal replacement. Eur J Cardiothorac Surg 9: 158–160, 1995
Larsen PN, Moesgaard F, Naver L, Rosenberg J, Gottrup F, Kirkegaard P, Helledie N: Gastric and colonic oxygen-tension measured with a vacuum-fixed oxygen-electrode. Scand J Gastroenterol 26: 409–418, 1991
Berliner LJ, Reuben J: In: Biological Magnetic Resonance: Spin Labeling-Theory and Applications. Plenum Press, New York and London, 1989
Sachse JH, Marsh D: Line intensities in spin-exchanged nitroxide ESR spectra. J Magn Reson 68: 540–543, 1986
Kuppusamy P, Zweier JL: A forward subtraction procedure for removing hyperfine artifacts in electron paramagnetic resonance imaging. Magn Reson Med 35: 316–322, 1996
Liu KJ, Miyake M, James PE, Swartz HM: Separation and enrichment of the active component of carbon based paramagnetic materials for use in EPR oximetry. J Magn Reson 133: 291–298, 1998
Swartz HM, Clarkson RB: The measurement of oxygenin vivousing EPR techniques. Phys Med Biol 43: 1957–1975, 1998
He G, Samouilov A, Kuppusamy P, Zweier JL:In vivoEPR imaging of the distribution and metabolism of nitroxide radicals in human skin. J Magn Reson 148: 155–164, 2001
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He, G., Samouilov, A., Kuppusamy, P., Zweier, J.L. (2002). In vivo imaging of free radicals: Applications from mouse to man. In: Vallyathan, V., Shi, X., Castranova, V. (eds) Oxygen/Nitrogen Radicals: Cell Injury and Disease. Developments in Molecular and Cellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1087-1_40
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DOI: https://doi.org/10.1007/978-1-4615-1087-1_40
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