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Mechanisms and Consequences of Proton Transport pp 233–245Cite as

Characterization, Regulation and Localization of the Gastric Cl- Channel Associated with Gastric Acid Secretion

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Abstract

The Cl-channel of the apical membrane of the gastric parietal cell was characterized in native rabbit H/K ATPase-containing membrane vesicles in bilayer reconstitution studies. It is a linear Cl- channel which exhibits activation by reduction of extracellular pH and PKA. The ClC-2 Cl- channel cloned from rabbit gastric mucosa and expressed in Xenopus laevis oocytes, exhibited similar electrophysiological characteristics and regulation as the native gastric Cl- channel. Potential PKA phosphorylation sites in rabbit ClC-2 appear to underlie PKA activation. The pH sensor on the extracellular surface of ClC-2 was identified as E419 and amidation resulted in activation of the channel. Recombinant ClC-2 stably expressed in HEK-293 cells showed Cl- currents that were activated by forskolin and IBMX, low extracellular pH, amidation, arachidonic acid and acid-activated omeprazole. Using immunofluorescent confocal microscopy, ClC-2 was localized to the secretory canalicular membrane of stimulated parietal cells and to intracellular structures (tubulovesicles) in resting parietal cells, which was confirmed by immunogold electron microscopy. Distribution of the gastric H/K ATPase and ClC-2 were similar. These findings strongly support the view that ClC-2 is the Cl- channel in the apical secretory membrane of the gastric parietal cell which, together and in concert with the H/K ATPase and a K+ channel, result in HCl secretion.

Keywords

  • Parietal Cell
  • Proton Transport
  • Gastric Parietal Cell
  • Physiol Cell
  • Immunofluorescent Confocal Microscopy

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Authors and Affiliations

  1. Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267-0576, USA

    Danuta H. Malinowska, Ann M. Sherry, Kirti P. Tewari & John Cuppoletti

Authors
  1. Danuta H. Malinowska
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  2. Ann M. Sherry
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  3. Kirti P. Tewari
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  4. John Cuppoletti
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Editor information

Editors and Affiliations

  1. Graduate School of Pharmaceutical Science, The University of Tokyo, Japan

    Tetsuro Urushidani

  2. Dept. of Molecular & Cell Biology, University of California at Berkeley, USA

    John G. Forte

  3. Center for Ulcer Research & Education, University of California, Los Angeles, USA

    George Sachs

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Malinowska, D.H., Sherry, A.M., Tewari, K.P., Cuppoletti, J. (2002). Characterization, Regulation and Localization of the Gastric Cl- Channel Associated with Gastric Acid Secretion. In: Urushidani, T., Forte, J.G., Sachs, G. (eds) Mechanisms and Consequences of Proton Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0971-4_27

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  • DOI: https://doi.org/10.1007/978-1-4615-0971-4_27

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