Photosensitization of Hepatitis A Virus and Other Non-Enveloped RNA Viruses by Meso-Substituted Porphyrins in Water and Human Plasma

  • Michael J. Casteel
  • Avram Gold
  • Mark D. Sobsey
  • Mark E. Brecher


Some viruses that cause disease in humans are transmissible by consumption of contaminated water or through the transfusion of blood or its products. Human viruses such as hepatitis A virus (HAV) and parvovirus B19 (PVB19) have caused disease or have been detected in drinking water9 or in blood products such as pooled plasma15 and clotting factor11,18,21. HAV and PVB19 are small (22–27 nm), non-enveloped human viruses that contain ribonucleic acid or deoxyribonucleic genomes, respectively. A residual risk of infection from these agents is always present, because the effectiveness of bairiers (e.g., blood donor exclusion17, microbiological testing, inactivation or treatment techniques14,16) against HAV and other viral pathogens for the prevention of contaminated blood products and water from reaching patients and consumers is finite. Non-enveloped viruses such as HAV are resistant to the removal and inactivation procedures used in both water safety19,25 and transfusion medicine16. Procedures in transfusion medicine with demonstrated efficacy against lipid-enveloped viruses such as the human immunodeficiency virus, and hepatitis B and C viruses3 are ineffective against HAV, PVB19, and perhaps other viruses with similar biological features. Hence, alternative methods for virus inactivation in water and in blood components are needed.


Virus Inactivation Cationic Porphyrin Factor Viii Concentrate Porphyrin Solution Similar Biological Feature 
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  1. 1.
    Adams, MH. Bacteriophages, Wiley-Interscience, New York, 1959.Google Scholar
  2. 2.
    Ben-Hur, E, Horowitz B. 1995. Advances in photochemical approaches for blood sterilization. Photochem Photobiol 1995; 62:383–388.PubMedCrossRefGoogle Scholar
  3. 3.
    Ben-Hur, E, Horowitz B. Virus inactivation in blood. AIDS 1996; 10:1183–1190.PubMedCrossRefGoogle Scholar
  4. 4.
    Benimetskaya, L, Takle GB, Vilenchik, M, Lebedeva I, Miller, P, Stein, CA. Cationic porphyrins: novel delivery vehicles for antisense oligodeoxynucleotides. Nucleic Acids Research 1998; 26(23):5310–7.PubMedCrossRefGoogle Scholar
  5. 5.
    Bonnett, R. Photosensitizers of the Porphyrin and Phtalocyanine Series for Photodynamic Therapy. Chem Soc Reviews 1995; 19–33.Google Scholar
  6. 6.
    Caughey, WS, Raymond LD, Horiuchi, M, Caughey, B. Inhibition of protease-resistant prion protein formation by porphyrins and phtalocyanines. Proc Natl Acad Sci USA 1998; 95:12117–12122.PubMedCrossRefGoogle Scholar
  7. 7.
    Chamberland, ME, Alter, HJ, Busch, MP, Nemo, G, Ricketts, M. Emerging Infectious Disease Issues in Blood Safety.Emerg Infect Dis 2001; 7(3).Google Scholar
  8. 8.
    Cromeans, T, Sobsey MD, Fields, HA. Development of a Plaque Assay for a Cytopathic, Rapidly Replicating Isolate of Hepatitis A Virus. J Med Virol 1987; 22:45–56.PubMedCrossRefGoogle Scholar
  9. 9.
    DeSerres, G, Cromeans TL, Levesque B, Brassard, N, Barthe, C, Dionne M, Prud’homme H, Paradis D, Shapiro CN, Nainan OV, Margolis HS. Molecular confirmation of hepatitis A virus from well water: epidemiology and public health implications. J Infect Dis 1999; 179(l):37–43.CrossRefGoogle Scholar
  10. 10.
    DiMauro, E., R. Saladino, P. Tagliaesta, V. De Sanctis, and R. Negri. 1998. Manganese Water-soluble porphyrin Senses DNA Conformation. J Molecular Biol 282:43–57.CrossRefGoogle Scholar
  11. 11.
    Erdman DD, Anderson BC, Torok TJ, Finkel TH, Anderson LJ. Possible transmission of parvovirus B19 from intravenous immune globulin. J Med Virol 1997; 53(3):233–6.PubMedCrossRefGoogle Scholar
  12. 12.
    Flynn SM, George ST, White L, Devonish W, Takle GB. Water-Soluble, Meso-Substituved Cationic Porphyrins — a Family of Compounds for Cellular Delivery of Oligonucleotides. BioTechniques 1999; 26:736–746.PubMedGoogle Scholar
  13. 13.
    Gerba CP, Wallis C, Melnick JL. Application of photodynamic oxidation to disinfection of tapwater, seawater, and sewage contaminated with poliovirus. Photochem Photobiol 1977; 26:499–504.PubMedCrossRefGoogle Scholar
  14. 14.
    Hilfenhaus J, Nowak, T. Inactivation of Hepatitis A Virus by Pasteurization and Elimination of Picornaviruses during Manufacture of Factor VIII Concentrate. Vox Sang 1994;67(suppll):62–66.PubMedCrossRefGoogle Scholar
  15. 15.
    Koenigbauer UF, Eastlund T, Day JW. Clinical illness due to parvovirus B19 infection after infusion of solvent/detergent-treated pooled plasma.Transfusion 2000; 40(10): 1203–6.PubMedCrossRefGoogle Scholar
  16. 16.
    Lemon SM, Murphy PC, Smith A, Zou J, Hammon J, Robinson S, Horowitz B. Removal/neutralization of hepatitis A virus during manufacture of high purity, solvent/detergent factor VIII concentrate. J Med Virol 1994; 43:44.PubMedCrossRefGoogle Scholar
  17. 17.
    Moor ACE, Dubbelman TMAR, VanSteveninck J, Brand, A. Transfusion-transmitted diseases: risks, prevention and perspectives. Eur J Haemotl 1999; 62:1–18.CrossRefGoogle Scholar
  18. 18.
    Normann A, Graff J, Gerritzen A, Brackmann HH, Flehmig B. Detection of hepatitis A virus RNA in commercially available factor VIII preparation. Lancet 1992; 340(14): 1232.PubMedCrossRefGoogle Scholar
  19. 19.
    Peterson DA, Hurley TR, Hoff JC, Wolfe, LG. Effect of chlorine treatment on infectivity of hepatitis A virus. Appl Envr Microbiol 1983; 45(l):223–7.Google Scholar
  20. 20.
    Priola SA, Raines A, Caughey WS. Porphyrin and Phtalocyanine Antiscrapie Compounds. Science 2000; 287:1503–1506.PubMedCrossRefGoogle Scholar
  21. 21.
    Robertson BH, Alter MJ, Bell BP, Evatt B, McCaustland KA, Shapiro CN, Sinha SD, Souci JM. Hepatitis A Virus Sequences Detected in Clotting Factor Concentrates Associated With Disease Transmission. Biologicals 1998; 26:95–99.PubMedCrossRefGoogle Scholar
  22. 22.
    Santus R, Gellier P, Schrevel J, Maziere JC, Stoltz JF. Photodecontamination of blood components: advantages and drawbacks. Clin Hemor Microcirc 1998; 18:299–308.Google Scholar
  23. 23.
    Sloand EM. Viral risks associated with blood transfusion. Photochem Photobiol 1997; 65(3):428–431.PubMedCrossRefGoogle Scholar
  24. 24.
    Sobsey MD, Carrick RJ, Jensen HR. Improved Methods for Detecting Enteric Viruses in Oysters. Appl Envr Microbiol 1978; 36:121.Google Scholar
  25. 25.
    Sobsey MD, Fuji T, Hall RM. Comparing Inactivation of Cell-Associated and Dispersed Hepatitis A Virus in Water by Free Chlorine and Monochloramine. J Am Water Works Assoc 1991; November issueGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Michael J. Casteel
    • 1
  • Avram Gold
    • 1
  • Mark D. Sobsey
    • 1
  • Mark E. Brecher
    • 2
  1. 1.Department of Environmental Sciences & Engineering, School of Public HealthUniversity of North Carolina at Chapel HillUSA
  2. 2.Department of Pathology and Laboratory MedicineUniversity of North Carolina Hospitals Chapel HillUSA

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