Ultraviolet a Radiation-Induced Signal Transduction in Human Keratinocytes

  • Jean Krutmann
  • Susanne Grether-Beck


The ultraviolet (UV) radiation solar spectrum that reaches the earth consists of short wavelength UVC (< 280 nm), intermediate wavelength UVB (280–320 nm) and long wavelength UVA (320–400 nm) radiation. Under physiological conditions, human cells are exclusively exposed to UVB and UVA radiation, because ozone in the stratosphere completely absorbs wavelengths < 290 nm. The amount of UVA radiation reaching the earth’s surface is approximately 20 times greater than that of UVB radiation, and solar UVA radiation contributes to photoageing and photocarcinogenesis of human skin and is responsible for triggering the most frequent photodermatoses, that is polymorphous light eruption (Urbach, 1992).


Singlet Oxygen Human Keratinocytes Rose Bengal Localize Scleroderma Urticaria Pigmentosa 
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  1. Basu-Modak, S. and Tyrrell, R.M. (1993) Singlet oxygen: A primary effector in the ultraviolet A/ near-visible light induction of the human heme oxygenase gene. Cancer Res. 53, 4505–4519.PubMedGoogle Scholar
  2. Bauer, R., Imhof, A., Pscherer, A., Kopp, H., Moser, M., Seegers, S., Kerscher, M., Tainky, M.A., Hofstaedter, F., Buettner, R. (1994). The genomic structure of the human AP-2 transcription factor. Nucl. Acids. Res. 22, 1413–1420.PubMedCrossRefGoogle Scholar
  3. Bender, K., Blattner, C., Knebel, A., Iordanov, M., Herrlich, P., Rahmsdorf, H.J. (1997). UV-induced signal transduction. Photochem. Photobiol B 37, 1–17.CrossRefGoogle Scholar
  4. Buettner, R., Kannan, P., Imhof, A., Bauer, R., Yim, S.O.,Glockshuber, R., van Dyke, M.W., Tainsky, M.A. (1993). An alternatively spliced mRNA from the AP-2 gene enodes a negative regulator of transcriptional activation by AP-2. Mol. Cell. Biol. 13, 4174–4185.PubMedGoogle Scholar
  5. Duan, C. and Clemmons, D.R. (1995). Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J. Biol. Chem. 270, 24844–24851.PubMedCrossRefGoogle Scholar
  6. Grether-Beck, S., Olaizola-Horn, S., Schmitt, H., Grewe, M., Jahnke, A., Johnson, J.P., Briviba, K., Sies, H., Krutmann, J. (1996). Activation of transcription factor AP-2 mediates UVA radiation-and singlet oxygen-induced expression of the human intercellular adhesion molecule 1 gene. Proc. Natl. Acad. Sci. USA 93:14586–14591.PubMedCrossRefGoogle Scholar
  7. Felsner, I., Grether-Beck, S., Schmitt-Brenden, H., Krutmann, J. (2001) Mitochondrial cytochrome C mediates ceramide-induced gene expression in human keratinocytes through oxidation of transcription factor AP-2. 295 Abstract. Arch. Dermatol. Res. 403, 30.Google Scholar
  8. Grether-Beck, S., Schmitt, H., Grewe, M., Buettner, R., Krutmann, J. (1997) The balance between expression of transcription factor AP2 and its alternative splice product AP2B controls ultraviolet A radiation (UVAR)-induced gene expression in human cells. J. Invest. Dermatol. 108, 594.Google Scholar
  9. Grether-Beck, S., Bonizzi, G., Schmitt-Brenden, H., Felsner, I., Timmer, A., Sies, H., Johnson, J.P., Piette, J., Krutmann, J. (2000) Non-enzymatic triggering of the ceramide signalling cascade by solar UVA radiation. EMBO J. 19, 5793–5800.PubMedCrossRefGoogle Scholar
  10. Karin, M., and Herrlich, P. (1989) Cis-and trans-acting elements responsible for induction of specific genes by tumor promotors, serum factors, and stress. In: Genes and Siganl Transduction in Multistage Carcinogenesis, Colburn, N.H., ed., Marcel Dekker, New York, pp. 415–440.Google Scholar
  11. Kochevar, I.E. (1995). Primary processes in photobiology and photosensitation. In: Photoimmunology. Krutmann, J., and Elmets, C.A., eds. (Blackwell Science Ltd.) pp. 19–33.Google Scholar
  12. Krutmann, J., Czech W, Diepgen T, Niedner R, Kapp A, Schöpf, E. (1992). High-dose UVA1 therapy in the treatment of patients with atopic dermatitis. J Am Acad Dermatol 26, 225–230.PubMedCrossRefGoogle Scholar
  13. Krutmann, J., and Grewe, M. (1995) Involvement of cytokines, DNA damage, and reactive oxygen intermediates in ultraviolet radiation-induced modulation of intercellular adhesion molecule-1 (ICAM-1) expression. J. Invest. Dermatol. 105, 67S–70S.PubMedCrossRefGoogle Scholar
  14. Morita, A., Grewe, M., Grether-Beck, S., Olaizola-Horn, S., Krutmann, J. (1997). Induction of proinflammatory cytokines in human epidermoid carcinoma cells by in vitro ultraviolet A1 irradiation. Photochem. Photobiol. 65, 630–635.PubMedCrossRefGoogle Scholar
  15. Morita, A., Werfel, T., Stege, H., Ahrens, C., Karmann, K., Grewe, M., Grether-Beck, S., Ruzicka, T., Kapp, A., Klotz, L.O., Sies, H., Krutmann, J. (1997) Evidence that singlet oxygen-induced human T-helper cell apoptosis is the basic mechanism of ultraviolet-A radiation phototherapy. J. Exp. Med. 186, 1763–1768.PubMedCrossRefGoogle Scholar
  16. Punchard, N.A., and Kelly, F.J. (1996). Free radicals. In: Free Radicals-A practical approach eds. N.A. Punchard and F.J. Kelly. (IRL press, Oxford, New York, Tokyo) pp. 1–8.Google Scholar
  17. Stege, H., Humke, S., Berneburg, M. et al., 1996. High-dose UVAl therapy in the treatment of patients with localized scleroderma (morphea). J. Am. Acad. Dermatol. 36, 938–944.CrossRefGoogle Scholar
  18. Stege H., Schöpf, E., Ruzicka, T., Krutmann, J. (1996). High-dose UVAl for urticaria pigmentosa. Lancet 347, 64.PubMedCrossRefGoogle Scholar
  19. Tyrrell, R.M. (1996). Activation of mammalian gene expression by the UV component of sunlight -from models to reality. Bioassays 18, 139–148.CrossRefGoogle Scholar
  20. Urbach, F., ed. (1992) Biological responses to ultraviolet A radiation. Valdenmar, Overland Park, Kansas.Google Scholar
  21. Wlaschek, M. Briviba, K., Stricklin, G.P., Sies, H., and Scharfetter-Kochanek, K. (1995). Singlet oxygen may mediate the ultraviolet A-induced synthesis of interstitial collagenase. J. Invest. Dermatol. 104, 194–198.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Jean Krutmann
    • 1
  • Susanne Grether-Beck
    • 1
  1. 1.Clinical and Experimental Photodermatology, Department of DermatologyHeinrich-Heine-UniversityDüsseldorfGermany

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