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Polyclonal Antisera Against Estuarine Crustacean Vitellins: A Molecular Approach to Reproductive Endocrinology and Toxicology

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Modern Approaches to the Study of Crustacea

Abstract

To fully elucidate the action of crustacean hormones, or their agonists, on vitellogenesis and reproduction, it has become increasingly important to develop sensitive assays that indicate a stimulatory or inhibitory effect on easily measured endpoints. Because of the relative abundance of vitellin in crustacean yolk and the ease with which it can be isolated, this protein makes an excellent model for studying the mechanisms that control and regulate reproduction. Vitellin was purified from eggs of adult female Lepidophthalmus louisianensis, Palaemonetes pugio, Rhithropanopeus harrisii, Americamysis (Mysidopsis) bahia, and Uca panacea which were collected from estuarine localities in Santa Rosa Sound, Gulf Breeze, Florida during late spring and summer of 1999. The purified proteins were used to immunize rabbits for polyclonal antibody production. Specificity of antisera was tested against respective vitellins by Western blotting. These antisera will be used to develop assays useful to the study of reproductive endocrinology, vitellogenesis, and toxicology.

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Authors and Affiliations

  1. Center for Environmental Diagnostics and Bioremediation, University of West Florida, Pensacola, Florida, 32514, USA

    Shea R. Tuberty

  2. Zeigler Bros., Inc., Gardners, Pennsylvania, 17324, USA

    Sergio F. Nates

  3. U.S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Breeze, Florida, 32561, USA

    Charles L. McKenney Jr.

Authors
  1. Shea R. Tuberty
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  2. Sergio F. Nates
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  3. Charles L. McKenney Jr.
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Editor information

Editors and Affiliations

  1. Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, México D.F., México

    Elva Escobar-Briones

  2. Instituto del Biología, Universidad Nacional Autónoma de México, México D.F., México

    Fernando Alvarez

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Tuberty, S.R., Nates, S.F., McKenney, C.L. (2002). Polyclonal Antisera Against Estuarine Crustacean Vitellins: A Molecular Approach to Reproductive Endocrinology and Toxicology. In: Escobar-Briones, E., Alvarez, F. (eds) Modern Approaches to the Study of Crustacea. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0761-1_5

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  • DOI: https://doi.org/10.1007/978-1-4615-0761-1_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5228-0

  • Online ISBN: 978-1-4615-0761-1

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