Potency and Duration of Action of Synthetic P2Y2 Receptor Agonists on Schirmer Scores in Rabbits

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 506)


P2Y2 receptors belong to the family of G-protein coupled receptors and are activated by nucleotides such as ATP and UTP.1,2 Activation of P2Y2 receptors (P2Y2-Rs) on various epithelial cell types enhances chloride, fluid and mucin secretion and thereby stimulates the body’s natural mucosal lubrication and clearance mechanisms.3,4 On the ocular surface, P2Y2-R mRNA is localized in various epithelial cells in the eyelid, cornea and conjunctiva. Previous work using conventional non-isotopic in situ hybridization techniques demonstrated localization of P2Y2-R mRNA in the palpebral conjunctiva of the eyelid, the epithelium of the lid margin and the meibomian gland; the corneal epithelium and adjacent bulbar conjunctiva, particularly the conjunctival goblet cells.5


Case Group Ocular Surface Back Surface Lesion Development Sodium Fluorescein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    V. Ralevic and G. Burnstock. Receptors for purines and pyrimidines. Pharmacol Rev. 50:413–792 (1998).PubMedGoogle Scholar
  2. 2.
    J.T. Turner, G.A. Weisman and J.S. Redan. (Eds.) The P2 nucleotide receptor. Humana Press: Totowa, New Jersey (1997).Google Scholar
  3. 3.
    S.J. Mason, A.M. Paradiso and R.C. Boucher. Regulation of transepithelial ion transport and extracellular ATP in human normal and cystic fibrosis airway epithelium. Br J Pharmacol. 103:1649–1656 (1991).CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    D. Drutz, C. Shaffer, K. LaCroix, et al. Uridine 5′-triphosphate (UTP) regulates mucociliary clearance via purinergic receptor activation. Drug Dev Res. 37:185 (1996).Google Scholar
  5. 5.
    B.R. Yerxa, V.Z. Zhang, L. Sheridan, et al. Cellular localization of P2Y2 receptor gene expression in primate ocular tissues by nonisotopic in situ hybridization. Invest Ophthalmol Vis Sci. 41:S540 Abstract #2630 (2000).Google Scholar
  6. 6.
    Y. Li, K. Kuang, Q. Wen, et al. Rabbit conjunctival epithelium transports fluid. Invest Ophthal Vis Sci. 40:S90 Abstract #482 (1999).Google Scholar
  7. 7.
    J.E. Jumblatt and M.M. Jumblatt. Regulation of ocular mucin secretion by P2Y2 nucleotide receptors in rabbit and human conjunctiva. Exp Eye Res. 67:341–346 (1998).CrossRefPubMedGoogle Scholar
  8. 8.
    B.R. Yerxa, P.P. Elena, T. Caillaud, et al. INS365, a P2Y2 receptor agonist, increases Schirmer scores in albino rabbits. Invest Ophthalmol Vis Sci. 40:S540 Abstract #2848 (1999).Google Scholar
  9. 9.
    W. Pendergast, B.R. Yerxa, J.G. Douglass III, R. W. Dougherty, A.C. Jones, G. Olins, K. Redick, J.L. Rideout and S.R. Shaver. Synthesis and P2Y receptor activity of a series of uridine dinucleoside 5’-polyphosphates. Bioorg Med Chem Lett. 11:157–160 (2001).CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 2002

Authors and Affiliations

  1. 1.Inspire PharmaceuticalsDurhamUSA
  2. 2.Iris PharmaLa GaudeFrance

Personalised recommendations