Tumor immunity in mice immunized with fibroblasts transfected with tumor DNA

  • Edwin F. de Zoeten
  • Donghua Li
  • Edward P. Cohen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 495)


Accumulating evidence from multiple sources’ indicates that cancer cells form unique tumor associated antigens (TAAs). Under appropriate circumstances, they can become the targets of immune-mediated attack. Immunization of tumor-bearing animals, and patients, with tumor vaccines can stimulate anti tumor immune responses23leading to tumor rejection.


Squamous Cell Carcinoma Cell Anti Tumor Immunogenic Property Melanoma Associate Antigen Small Tumor Burden 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Boon, T., Cerottini, J-C, Van den Eynde, B., van der Bruggen, P. And Van Pel, A. Tumor antigens recognized by T lymphocytes. Annul Rev Immunol 12: 337–361, 1994CrossRefGoogle Scholar
  2. 2.
    Boon T. Tumor antigens recognized by cytolytic T lymphocytes: present perspectives for specific immunotherapy. Int. J. Cancer54: 177–180, 1993.PubMedCrossRefGoogle Scholar
  3. 3.
    Storkus, W.J., Zeh, H.J. III, Maeurer, MJ., Salter, R.D. and Lotze, M.T. Identification of human melanoma peptides recognized by class I restricted tumors infiltrating T lymphocytes. J. Immunol. 151: 3719–3727, 1993PubMedGoogle Scholar
  4. 4.
    Cavallo, F., Pierro, F.D., Giovarelli, M., Gulino, A., Vacca, A., Stoppacciaro, A., Fomi, M., Modesti, A., and Fomi, G. Protective and curative potential of vaccination with interleukin-2-gene-transfected cells from a spontaneous mouse mammary adenocarcinoma. Cancer Res. 53: 5067–5070, 1993.PubMedGoogle Scholar
  5. 5.
    Golumbek, P.T., Lazenby, A.J., Levitsky, H.I., Jaffee, L.M., Karasuyama, H., Baker, M., and Pardoll, D.M. Treatment of established renal cancer by tumor cells engineered to secrete interleukin-4. Science 254: 713–716, 1991.PubMedCrossRefGoogle Scholar
  6. 6.
    Mullen, C.A., Coale, M.M., Levy, A.T., Stetler-Stevenson, W.G., Liotta, L.A., Brandt, S., and Blaese, R.M. Fibrosarcoma cells transduced with the IL-6 gene exhibit reduced tumorigenicity, increased immunogenicity, and decreased metastatic potential. Cancer Res. 52: 6120–6124, 1992.Google Scholar
  7. 7.
    Townsend S. E. and Allison, J.P. 1993. Tumor rejection after direct costimulation of CD8+T cells by B7 transfected melanoma cells Science 259:368.Google Scholar
  8. Geraghty PJ, Fields RC, Mule JJ. Vaccination with tumor-pulsed splenic dendritic cells mediates immunity to poorly-immunogenic tumor. Surg. Forum 47: 459–461, 1996.Google Scholar
  9. 9.
    Mayordomo JI, Zorina Y, Storkus WJ, Zitvogel L, Celluzzi C, et al Bone marrow derived dendritic cells pulsed with synthetic tumor peptides elicit protective and therapeutic anti turno rimmunity. Nat Med 1: 1297–1302, 1995PubMedCrossRefGoogle Scholar
  10. 10.
    Palgia P, Chiodoni C, Rodolfo M and Colombo MP. Murine dendritic cells loaded in vitro with soluble protein prime cytotoxic T lymphocytes against tumor antigen in vivo. J Exp Med 183: 317–322, 1996CrossRefGoogle Scholar
  11. 11.
    Boczkowski D, Nair SK, Nam JH, Lyerly HK, Gilboa E. Induction of tumor immunity and cytotoxic T lymphocyte responses using dendritic cells transfected with messenger RNA amplified from tumor cells. Cancer Res. 2000 Feb 15;61(4):1028–34.Google Scholar
  12. 12.
    Kim, T.S., Russell, S.J., Collins, M.K.L., and Cohen, E.P. Immunity to B16 melanoma in mice immunized with IL-2-secreting allogeneic mouse fibroblasts expressing melanoma associated antigens. Int. J. Cancer 51: 283–289, 1992PubMedCrossRefGoogle Scholar
  13. 13.
    Kim, T. S. and E. P. Cohen. 1994. Interleukin-2-secreting mouse fibroblasts transfected with genomic DNA from murine melanoma cells prolong the survival of mice with melanoma.Cancer Research54 (May):2531–2535.PubMedGoogle Scholar
  14. 14.
    deZoeten, E.F., Carr-Brendel, V., Cohen, E.P. Resistance to melanoma in mice immunized with semiallogeneic fibroblasts transfected with DNA from mouse melanoma cells.J. Immuno161: 2915–2922, 1998.Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Edwin F. de Zoeten
    • 1
  • Donghua Li
    • 1
  • Edward P. Cohen
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of IllinoisChicagoIllinois

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