Oxygenation of Arachidonic Acid by Cyclooxygenases Generates Reactive Intermediates That Form Adducts with Proteins
Cyclooxygenase-2 (COX-2) catalyzes the oxygenation of arachidonic acid into the prostaglandin endoperoxide, PGH2. PGH2 is then processed by different isomerases into prostaglandins and thromboxane A2. PGH2 also undergoes rearrangement in aqueous solution into PGE2, PGD2 and into the levuglandins (LG) E2 and D2 1. LG’s are highly reactive γ-ketoaldehydes that have been shown to form adducts and cross-links with proteins 2, 3, primarily through their reaction with the ε-amine of lysine. The potential that these levuglandin adducts of proteins could be biologically important may be inferred from the knowledge that lipid-modification of proteins is known to influence their function and cellular localization 4–7.
KeywordsArachidonic Acid Covalent Binding Adduct Formation Sinapinic Acid Prostaglandin Endoperoxide
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