Abstract
The variability associated with testing wheat for deoxynivalenol (DON) was measured using a 0.454 kg sample, a Romer mill, 25 g of comminuted subsample and the Romer Fluoroquant analytical method. The total variability was partitioned into sampling, sample preparation, and analytical variability components. Each variance component was found to be a function of the DON concentration and equations were developed to predict each variance component using regression techniques. The effects of sample size, subsample size, and number of aliquots on reducing the variability of the DON test procedure were also determined. Using the test procedure described above, the coefficient of variation (CV) associated with testing wheat at 5 ppm DON was found to be 13.4%. The CVs associated with sampling, sample preparation, and analysis were 6.3, 10.0, and 6.3%, respectively. The sample variations associated with testing wheat are relatively small when compared to CVs associated with testing other commodities for other mycotoxins such as aflatoxin in peanuts. Even with the use of a small sample size (0.454 kg), the sampling variation was not the largest source of error as found in other mycotoxin test procedures.
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References
Food and Agriculture Organization, 1995, Worldwide Regulations for MycotoxinsFAO Food and Nutrition Paper 64.Food and Agriculture Organization of the United Nations, Vialle delle Terme di Caracalla, 00100 Rome, Italy, pp. 43.
Hart, L.P. and Schabenberger, O., 1998, Variability of vomitoxin in truckloads of wheat in awheat scab epidemic yearPlant Disease.82:625–630.
Malone, B.L., Humphrey, C.W., Romer, T.R., and Richard, J.L., 1998, One-step solid-phase cleanup and fluorometric analysis of deoxynivalenol in grains J. Assoc. Offic Anal. Chem., Inc. 82:448–452.
Mirocha, C.J., Pathre, S.V., Schauerhamer, B., and Christensen, C.M., 1976, Natural occurrence of fusarium toxins in feedstuffsAppl. Environ. Microbiol.32:553.
Statistical Analysis System Institute, Inc., 1997SAS/STAT Software: Changes and Enhancements through Release 6.12SAS Institute, Inc., Cary, NC 27511.
Vesonder, R.F. and Hesseltine, C.W., 1981, Vomitoxin: Natural occurrence on cereal grains and significance as a refusal and emetic factor to swineProcess Biochem.16:12–15.
Whitaker, T.B., Dowell, F.E., Hagler, W.M. Jr., Giesbrecht, F.G. and Wu, J., 1994, Variability associated with sampling, sample preparation, and chemical testing for farmers’ stock peanuts J. Assoc.Off. Anal. Chem., Int. 77:107–116.
Whitaker, T.B., Giesbrecht, F.G., and Wu, J., 1996, Suitability of several statistical models to simulate observed distribution of sample test results in inspection of aflatoxin-contaminated peanut lotsJ. Assoc. Official Analytical Chem. Int. 79:981–988.
Whitaker, T.B., Dickens, J.W. and Monroe, R.J., 1979, Variability associated with testing corn for aflatoxinJ. Amer. Oil Chem. Soc.56:789–794.
Whitaker, T.B, Dickens, J.W. and Monroe, R.J., 1972, Comparison of the observed distribution of aflatoxin in shelled peanuts to the negative binomial distributionJ Amer. Oil Chem. Soc.49:590–593.
Whitaker, T.B., Dickens, J.W. and Monroe, R.J., 1974, Variability of aflatoxin test results, J.Amer. Oil Chem.Soc.51:214–218 (1974).
Whitaker, T.B., Whitten, M.E. and Monroe, R.J., 1976, Variability associated with testing cottonseed for aflatoxinJ. Amer. Oil Chem. Soc.53:502–505.
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© 2002 Springer Science+Business Media New York
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Whitaker, T.B., Hagler, W.M., Giesbrecht, F.G., Johansson, A.S. (2002). Sampling Wheat for Deoxynivalenol. In: DeVries, J.W., Trucksess, M.W., Jackson, L.S. (eds) Mycotoxins and Food Safety. Advances in Experimental Medicine and Biology, vol 504. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0629-4_8
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DOI: https://doi.org/10.1007/978-1-4615-0629-4_8
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