Abstract
The official patulin LC procedure was further examined (AOAC 995.10). Juice or juice concentrate was extracted with ethyl acetate and cleaned up with sodium carbonate. Patulin in the dried extract was determined by reversed-phase LC with UV detection (280 nm) in 1% THE aqueous solution after evaporation of the ethyl acetate. An end-capped C18 column was required to separate patulin from hydroxymethylfurfural. Patulin was detected in approximately half of the >1000 extracts examined. Only ca 10% of the extracts contained patulin at levels greater than 50 µg/L (50 ppb). Some presumptive findings were confirmed by capillary gas chromatography/mass spectrometry as the trimethyl silyl derivative using electron ionization or as underivatized patulin using negative ion chemical ionization. Trifluoropropylmethyl polysiloxane capillary columns provided superior gas chromatography of underivatized patulin compared to phenyl/methyl polysiloxane and methyl polysiloxane columns.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brause, A., Trucksess, M., Thomas, F., Page, S., 1996, Determination of patulin in apple juice by liquid chromatography: collaborative study, J. Assoc. Off Anal. Chem. 79:451.
Forbito, P.R., Babsky, N.E., 1985, Rapid liquid chromatographic determination of patulin in apple juice, J. Assoc. Off. Anal. Chem. 68:950.
Official Methods of Analysis, 1995, 16t Ed. March 1996 Supplement, AOAC International, Gaithersburg, MD, sec 995.10.
Rails, J.W., Lane, R.M., 1977, Examination of cider vinegar for patulin using mass spectrometry, J. Food Sci. 42:1117.
Roach, J.A.G., White, K.D., Trucksess, M.W., Thomas, F.W., 2000, Capillary gas chromatography/mass spectrometry with chemical ionization and negative ion detection for confirmation of identity of patulin in apple juice, Int. J. Assoc. Off Anal. Chem. 83:104.
Rupp, H.S., Turnipseed, S.B., 2000, Confirmation of patulin and 5-hydroxymethylfurfural in apple juice by gas chromatography/mass spectrometry, Int. J Assoc. Off Anal. Chem. 83:612.
Rychlik, M., Schierberle, P., 1998, Synthesis of “C-labeled patulin [4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one] to be used as internal standard in a stable isotope dilution assay, J. Agric. Food Chem. 46:5163.
Rychlik, M., Schierberle, P., 1999, Quantification of the mycotoxin patulin by a stable isotope dilution assay, J. Agric. Food Chem. 47:3749.
Stray, H., 1978, High pressure liquid chromatographic determination of patulin in apple juice, J. Assoc. Off. Anal. Chem. 61:1359.
Van Egmond, H., 1989, Current situation on regulations for mycotoxins. Overview of tolerances and status of standard methods of sampling and analysis, Food Addit. Contain. 6:139.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Science+Business Media New York
About this chapter
Cite this chapter
Roach, J.A.G., Brause, A.R., Eisele, T.A., Rupp, H.S. (2002). HPLC Detection of Patulin in Apple Juice with GC/MS Confirmation of Patulin Identity. In: DeVries, J.W., Trucksess, M.W., Jackson, L.S. (eds) Mycotoxins and Food Safety. Advances in Experimental Medicine and Biology, vol 504. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0629-4_13
Download citation
DOI: https://doi.org/10.1007/978-1-4615-0629-4_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5166-5
Online ISBN: 978-1-4615-0629-4
eBook Packages: Springer Book Archive