Boron in Plant Reproduction

  • B. Dell
  • L. Huang
  • R. W. Bell


In the period since the first B symposium in 1997 (Bell and Rerkasem 1997; Dell et al., 1997), progress in defining either structural or metabolic roles for B in reproductive parts of seed plants has been disappointing. However, better control of B in solution (Huang et al., 1999) has allowed B effects on anther and pollen development in wheat to be more fully documented (Huang et al., 2000, Huang et al., 2001). This work on wheat is briefly reviewed to highlight principles that are emerging about the function of B in reproductive development.


Pollen Tube Pollen Development Flag Leaf Pollen Growth Plant Reproduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ahn, A. and Jarvis, B.C., 1988, Effects of auxin and boron on nucleic acid metabolism and cell division during adventitious root regeneration. New Phytol. 108, 383–391.CrossRefGoogle Scholar
  2. Bell, R.W. and Rerkasem, B. (eds), 1997, Boron in Soils and Plants. Kluwer Academic Publishers, Dordrecht.Google Scholar
  3. Blackmore, S. and Barnes, S.H., 1990, Pollen wall development in angiosperms. In Blackmore S, Knox RB eds Microspores: evolution and ontogeny. Academic Press, London pp 173–192.Google Scholar
  4. Blevins, D.G. and Lukasewski, K.M., 1998, Boron in plant structure and function. Ann. Rev. Plant Physiol. Plant MoL Biol. 49, 481–500.CrossRefGoogle Scholar
  5. Busby, C.H. and O’Brien, T.P., 1979,. Aspects of vascular anatomy and differentiation of vascular tissues and transfer cells in vegetative nodes of wheat. Aust. J. Biol. 27, 703–711.CrossRefGoogle Scholar
  6. Chapman, G.P., 1987, The tapetum. Int. Rev. Cytol. 107, 111–125.CrossRefGoogle Scholar
  7. Clement, C. and Pacini, E., 2000, Anther plastids in angiosperms. Bot. Rev. 67, 54–73.CrossRefGoogle Scholar
  8. Clement, C., Mischler, P., Burrus, M., and Audran, J.C., 1997a, Characteristics of the photosynthetic apparatus and C02-fixation in the flower bud of Lilium. 1. Corolla. Int. J. Plant Sci. 158,794–800.CrossRefGoogle Scholar
  9. Clement, C., Mischler, P., Burru,S M., and Audran, J.C., 1997b, Characteristics of the photosynthetic apparatus and C02-fixation in the flower bud of Lilium. 2. Anther. Int. J. Plant Sci. 158:801–810.CrossRefGoogle Scholar
  10. Dell, B. and Huang, L., 1997, Physiological response of plants to low boron. Plant Soil 193: 103–120.CrossRefGoogle Scholar
  11. Dell, B., Brown, .PH., and Bell, R.W. (eds.), 1997, Boron in Soils and Plants: Reviews. Kluwer Academic Publishers, Dordrecht.Google Scholar
  12. Dorian, S., Lalonde, S., and Saini, H.S., 1996, Induction of male sterility in wheat by meiotic-stage water deficit is preceded by a decline in invertase activity and changes in carbohydrate metabolism in anthers. Plant Physiol. 111: 137–145.Google Scholar
  13. Feller, U., 1989, Transfer of rubidium from the xylem to the phloem in wheat internodes. J. Plant Physiol. 133:764–767.CrossRefGoogle Scholar
  14. Fleischer, A., O’Neill, M.A., and Ehwald, R., 1999, The pore size of non-graminaceous plant cell walls is rapidly decreased by borate ester cross-linking of the pectic polysaccharide rhamnogalacturonan II. Plant Physiol. 121: 829–838.PubMedCrossRefGoogle Scholar
  15. Geitmann, A., Hudák, J., Vennigerholz, F., and Walles, B., 1995, Immunogold localization of pectin and callose in pollen grains and pollen tubes of Brugsmansia suaveolens - Implications for the self-incompatibility reaction. J.Plant Physiol. 147: 225–235.CrossRefGoogle Scholar
  16. Goldbach, H.E., Yu, Q., Wingender, R., Schulz, M., Wimmer, M., Findeklee, P., and Baluska, F., 2001, Rapid response reactions of roots to boron deprivation. J. Plant Nutr. Soil Sci. 164: 173–181.CrossRefGoogle Scholar
  17. Hasegawa, Y., Nakamura, S., Uheda, E. and Nakamura, N., 2000, Immunolocalization and possible roles of pectins during pollen growth and callose plug formation in angiosperms. Grana 39: 46–55.CrossRefGoogle Scholar
  18. Huang, L., Bell, R.W. and Dell, B., 2001a, Boron supply into wheat (Triticum aestivum L. cv. Wilgoyne) ears whilst still enclosed within leaf sheaths. J. Exp. Bot. 52: 1–8.CrossRefGoogle Scholar
  19. Huang, L., Bell, R.W. and Dell, B., 1999, Factors controlling equilibrium boron (B) concentration in nutrient solution buffered with B-specific resin (Amberlite IRA-743). Plant Soil 208: 233–241.CrossRefGoogle Scholar
  20. Huang, L., Dell, B., and Bell, R.W., 2001b, Seasonal Conditions Modify Pollen Viability Responses to B Deficiency in Wheat (Triticum aestivum L. cv. Wilgoyne). Boron Conference, Bonn.Google Scholar
  21. Huang, L., Pant, J., Dell, B., and Bell, R.W., 2000, Effects of boron deficiency on anther development and floret fertility in wheat (Triticum aestivum L. ’Wilgoyne“). Ann. Bot. 85: 493–500.CrossRefGoogle Scholar
  22. Huang, L., Pant, J., Bell, R.W., Dell, B., and Deane, K., 1996, Effects of boron deficiency and low temperature on wheat sterility. In: Rawson HM, Subedi KD, eds.. Sterility in wheat in subtropical Asia: extent, causes and solutions. ACIAR Proceedings No. 72, 90– 101.Google Scholar
  23. Jackson, J.F., 1989, Borate control of protein secretion from Petunia pollen exhibits critical temperature discontinuities. Sex Plant Reprod. 2: 11–14.CrossRefGoogle Scholar
  24. Jackson, J.F., 1991, Borate control of energy-driven protein secretion from pollen and interaction of borate with auxin or herbicide - a possible role for boron in membrane events. Curr. Topics Plant Biochem. Physiol. 10: 221–229.Google Scholar
  25. Jewell, A.W., Murray, B.G., Alloway, B.J., 1988, Light and electron microscope studies on pollen development in barley (Hordeum vulgare L.) grown under copper-sufficient and deficient conditions. Plant Cell Env. 11: 273–281.CrossRefGoogle Scholar
  26. Kirkby, E.L.M. and Rymer, J.L., 1974, Development of the vascular system in the ear of barley. Ann. Bot. 38:565–573.Google Scholar
  27. Kirichenko, E.B., Krendéléva, T.E., Kukarskikh, G.P., and Nizovskaya, N.V., 1993, Photochemical activity in chloroplasts of anthers and caryopsis in cereals. Russ. Plant Physiol. 40: 229–233.Google Scholar
  28. Kobayashi, M., Matoh, T., and Azuma, J. 1996, Two chains of rhamnogalacturonan-II are cross-linked by borate-diol ester bonds in higher plant cell walls. Plant Physiol. 110: 1017– 1020.PubMedGoogle Scholar
  29. Kobayashi, M., Nakagawa, H., Asaka, T., and Matoh, T., 1999, Borate-rhamnogalacturonan II bonding reinforced by Ca2+ retains pectic polysaccharides in higher-plant cell walls. Pl. Physiol. 119: 199–203.CrossRefGoogle Scholar
  30. Kuppelwieser, H. and Feller, U., 1991, Transport of Rb and Sr to the ear in mature, exercised shoots of wheat: effects of temperature and stem length on Rb removal from the xylem. Plant Soil 132: 281–288.Google Scholar
  31. Lalonde, S., Beebe, D.U., and Saini, H.S., 1997, Early signs of disruption of wheat anther development associated with the induction of male sterility by meiotic-stage water deficit. Sex Plant Reprod. 10: 40–48.CrossRefGoogle Scholar
  32. Lalonde, S., Morse, D., and Saini, H.S., 1997, Expression of a wheat ADP-glucose pyrophosphorylase gene during development of normal and water-stress-affected anthers. PL Mol. Biol. 34: 445–453.CrossRefGoogle Scholar
  33. Matoh, T., 1997, Boron in plant cell walls. Plant Soil 193: 59–70.CrossRefGoogle Scholar
  34. Matoh, T. and Kobayashi, M., 1998, Boron and calcium, essential inorganic constituents of pectic polysaccharides in higher plant cells. J. Plant Res. 111: 179–190.CrossRefGoogle Scholar
  35. Matoh, T., Takasaki, M., Takabe, K., and Kobayashi, M., 1998, Immunocytochemistry of rhamnogalacturonan II in cell walls of higher plants. Plant Cell Biol. 39: 483–491.Google Scholar
  36. McCormick, S., 1993, Male gametophyte development. Plant Cell 5: 1265–1275.PubMedGoogle Scholar
  37. Mi, G.H., Liang, Z.X. and Mei, N., 1995, Studies on the effect of the formation of the ear-stem vascular connection structure on the growth and development of the young ear in winter wheat. Acta Agronomica Sinica 21: 210–214.Google Scholar
  38. Mogami, N., Nakamura, S. and Nakamura, N., 1999, Immunolocalization of the cell wall components in Pinus densiflora pollen. Protoplasma 206: 1–10.CrossRefGoogle Scholar
  39. Namuco, O.S. and O’Toole, J.C., 1986, Reproductive stage water stress and sterility. I. Effect of stress during meiosis. Crop Sci. 26: 317–321.CrossRefGoogle Scholar
  40. O’Neill, M., Warrenfeltz, D., Kates, K., Pellerin, P., Doco, T., Darvill, A.G., and Albersheim, P., 1996, Rhamnogalacturonan-II, a pectic polysaccharide in the walls of growing plant cell, forms a dimer that is covalently cross-linked by a borate ester. J. Biol. Chem. 271: 22923–22930.CrossRefGoogle Scholar
  41. Owen, H.A. and Makaroff, C.A., 1995, Ultrastructure and microsporogenesis in Arabidopsis thaliana (L.) Heynh. ecotype Wassilewskija (Brassicaceae). Protoplasma 185: 7–21.CrossRefGoogle Scholar
  42. Pacini, E., 1994, Cell biology of anther anther and pollen development. In: Williams E, Clark A, Knox RB, eds. Genetic control of self-incompatibility and reproductive development in flowering plants. Kluwer, Dordrecht pp 289–308.Google Scholar
  43. Pacini, E., 1996, Types and meaning of pollen carbohydrate reserves. Sex Plant Reprod. 9: 362–366.CrossRefGoogle Scholar
  44. Pacini, E., Franchi, G.G., and Hesse, M., 1985, The tapetum: its form, function, and possible phylogeny in Embryophyta . PL Syst. Evol. 149: 155–185.CrossRefGoogle Scholar
  45. Pizzolato, T.D., 1998, Procambial initiation for the vascular system in the spikelet of wheat Int. J. Plant Sci. 159:46–56.CrossRefGoogle Scholar
  46. Rawson, H.M., 1996, The developmental stage during which boron limitation causes sterility in wheat genotypes and the recovery of fertility. Aust. J. Plant Physiol. 23: 709–717.CrossRefGoogle Scholar
  47. Rerkasem, B., Lordkaew, S., and Dell, B., 1997, Boron requirement for reproductive development in wheat Soil Sci. Plant Nutr. 43: 953–957.Google Scholar
  48. Rhee, S.Y. and Somerville, C.R., 1998, Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall. Plant J. 15: 79–88.PubMedCrossRefGoogle Scholar
  49. Saini, H.S. and Aspinall, D., 1981, Effect of water deficit on sporogenesis in wheat (Triticum aestivum L.). Ann. Bot. 48: 623–633.Google Scholar
  50. Saini, H.S., Sedgley, M., and Aspinall, D., 1984, Developmental anatomy in wheat of male sterility induced by heat stress, water deficit or abscisic acid. Aust. J. Plant Physiol. 11: 243–253.CrossRefGoogle Scholar
  51. Sheoran, I.S. and Saini, H.S., 1996, Drought-induced male sterility in rice: changes in carbohydrate levels and enzyme activities associated with the inhibition of starch accumulation in pollen. Sex Plant Reprod. 9: 161–169.CrossRefGoogle Scholar
  52. Wakeley, P.R., Rogers, H.J., Rozycka, M., Greenland, A.J., and Hussey, P.J., 1998, A maize pectin methylesterase-like gene, ZmC5, specifically expressed in pollen. Pl. Mol. Biol. 37: 187–192.CrossRefGoogle Scholar
  53. Weir, C.E. and Dale, H.M., 1960, A developmental study of wild rice Zizania aquatica. Can. J. Bot. 38: 719–739.CrossRefGoogle Scholar
  54. Weirman, R. and Gubatz, S., 1992, Pollen wall and sporopollenin. Int. Rev. Cytol. 140: 35– 72.CrossRefGoogle Scholar
  55. Xu, H., Huang, Q., Shen, K., and Shen, Z., 1993, Anatomical studies on the effects of boron on the development of stamen and pistil of rape (Brassica napus). Acta Bot. Sinica 35: 453–457.Google Scholar
  56. Yang, X.D., Sun, S.Q. and Li, Y.Q., 1999, Boron deficiency causes changes in the distribution of major polysaccharides of pollen tube wall. Acta Bot. Sinica 41: 1169–1176.Google Scholar
  57. Zhang, X.S., Shen, Z.G., and Shen, K., 1994, Effect of boron on floral organ development and seed-setting of rapeseed. Acta Ped. Sinica 31: 146–152.Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • B. Dell
    • 1
  • L. Huang
    • 1
  • R. W. Bell
    • 1
  1. 1.Division of Science and EngineeringMurdoch University, MurdochPerthAustralia

Personalised recommendations