Arabidopsis thaliana Mutant bor1-1 is Defective in Boron Translocation

  • Junpei Takano
  • Kyotaro Noguchi
  • Hiroaki Hayashi
  • Toru Fujiwara

Abstract

A molecular genetic approach is potentially powerful to elucidate the molecular mechanisms underlying B uptake, translocation, and utilisation. The Arabidopsis thaliana mutants bor1–1 and bor1–2 require high levels of B for normal growth (Noguchi et al. 1997, Yasumori et al. 1999). Physiological characterisation of the mutant revealed important aspects of B physiology in plants. Along this line of research, we recently identified novel mutant lines which are related to boron nutrition. Line 8–21 was identified from EMS mutagenised Col-0 wild-type plants. The line shows growth retardation in shoots but not in root under low concentration of B supply (Aoki et al. 2001). Line 7A was also identified from EMS mutagenised Col-0 wild-type plants. The line exhibits growth retardation in shoots and root under low concentration of B supply (Takano et al. 2001a). Growth of these two newly isolated mutant lines were similar to the wild-type plants under sufficient supply of B, suggesting that growth defects are specifically related to B nutrition. We also identified another mutant line #11 from mutagenised bor1–1 mutant plants. The line #11 grows better than the bor1–1 mutant plants under moderate levels of B supply. B contents in the line #11 seems to be similar to those of bor1–1 mutant plants, suggesting that the line carries a mutation(s) that allows efficient use of B for growth (Fujiwara et al. 2001).

Keywords

Sucrose Boron Bori Galacturonan Rhamno 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Junpei Takano
    • 1
  • Kyotaro Noguchi
    • 1
  • Hiroaki Hayashi
    • 1
  • Toru Fujiwara
    • 1
  1. 1.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoYayoi, Bunkyo-ku, TokyoJapan

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