Gene Expression Changes and Progression to a BBB Phenotype in a Dynamic Model of the BBB
It is well established that mammalian endothelial cells (EC) acquire specialized functions in response to a variety of cellular, molecular and physical stimuli. While for most cell types, chemical signals play a pivotal, if not unique, role in determining cell physiology. Vascular EC differentiation depends on simultaneous exposure to both cellular (vascular smooth muscle, glia) and physical (shear stress, cyclical strain) signals. Shear stress in particular has been shown to cause profound changes in EC morphology ( Ott et al.,1995), gene expression (Akimoto et al.,2000), and function (Ngai and Winn, 1995). Some of these changes share similarities with EC responses to inflammatory cytokines. Thus, hemodynamic forces affecting ECs may play a role in both the development and prevention of vascular disease (Pohlman and Harlan, 2000).
KeywordsIschemia Lactate Pyruvate Luminal NADH
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