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The Physiological Genomics of the Critically Ill Mouse pp 73–88Cite as

Optical Spectroscopy in Mice

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Part of the book series: Basic Science for the Cardiologist ((BASC,volume 16))

Abstract

The major role of genetically engineered mice in cardiovascular research can hardly be overlooked. At present, part of the research on molecular biology is shifted from the cellular level to the level of intact animals. However, the researcher wanting to study the influence of molecular perturbations on the level of integrated physiology in mice is faced with major challenges. Now suddenly the advantages of using mice, i.e. small size and fast breeding speed, are becoming a burden to the researcher. This is especially true in cardiovascular research where emphasis lies on measurements of hemodynamic parameters in vivo.Here, many standard operations and measurement methods, used in larger animals, prove to be useless in mice because of small size and circulating blood volume. For example, taking standard blood samples of 0.2 ml appears to have major impact on blood pressure and microvascular oxygen levels’. Also the use of anesthesia and its various effects in different strains of mice can have a major impact on the results.

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Reference

  1. Van Bommel J, Maas MAW, Sinaasappel M, and Ince C. Intestinal microvascular P02 measurement with Pd-porphyrin phosphorescence in the mechanically ventilated mouse.Ad. Exp Med Bio1454: 189–193, 1998.

    Article  Google Scholar 

  2. Zuurbier CJ, Emons VM, and Ince C. Hemodynamics of anesthetized ventilated mouse models: aspects of anesthetics, fluid support, and strain. Am J Physiol 282: H2099–H2105, 2002.

    Google Scholar 

  3. Dumont EA, Reutelingsperger CPM, Smits JFM, Daemen MJAP, Doevendans PAF, Wellens HJJ, and Hofstra L. Real-time imaging of apoptotic cell-membrane changes at the single-cell level in the beating murine heart. Nat Med 7: 1352–1355, 2001.

    Article  PubMed  CAS  Google Scholar 

  4. Carlsen H, Moskaug JO, Fromm SH, and Blomhoff R. In vivo imaging of NF-xB activity. J Immunol 168: 1441–1446, 2002.

    PubMed  CAS  Google Scholar 

  5. Budinger TF, Benaron DA, and Koretsky AP. Imaging transgenic animals. Annu Rev Biomed Eng 01: 611–648, 1999.

    Article  CAS  Google Scholar 

  6. Balaban RS and Hampshire VA. Challenges is small animal noninvasive imaging. ILAR J 42: 248–262, 2001.

    PubMed  CAS  Google Scholar 

  7. Shonat RD, Wachman ES, Niu W, Koretsky AP, and Farkas DL. Near-simultaneous hemoglobin saturation and oxygen tension maps in mouse brain using an AOTF microscope.BiophysJ 73:1223–1231, 1997.

    Article  CAS  Google Scholar 

  8. Baxter WT, Davidenko JM, Loew LM, Wuskell JP, and Jalife J. Technical features of a CCD video camera system to record cardiac fluorescence data. Ann Biomed Eng 25: 713–725, 1997.

    Article  PubMed  CAS  Google Scholar 

  9. Green TJ, Wilson DF, Vanderkooi JM, and DeFeo SP. Phosphorimeters for analysis of decay profiles and real time monitoring of exponential decay and oxygen concentrations.Anal Biochem174: 73–79, 1988.

    Article  PubMed  CAS  Google Scholar 

  10. Mik EG, Donkersloot C, Raat NJH, and Ince C. Excitation pulse deconvolution in luminescence lifetime analysis for oxygen measurements in vivo. Photochem Photobiol 76: 12–21, 2002.

    Article  PubMed  CAS  Google Scholar 

  11. Coremans JMCC, Ince C, and Bruining HA. NADH fluorimetry and diffuse reflectance spectroscopy on rat heart. In:Medical Optical Tomography: Functional Imaging and Monitoring.Edited by Müller et al. Washington, SPIE-The International Society for Optical Engineering, p. 589–617, 1993.

    Google Scholar 

  12. Ince C, Coremans JM, and Bruining HA. In vivo NADH fluorescence. Adv Exp Med Biol 317: 277–296, 1992.

    Article  PubMed  CAS  Google Scholar 

  13. Chance B, Leigh JS, Miyake H, Smith DS, Nioka S, Greenfeld R, Einander M, Kaufmann K, Levy W, Young M, Cohen P, Yoshioka H, and Boretsky R. Comparison of time-resolved and -unresolved measurements of deoxyhemoglobin in brain. Proc Natl Acad Sci 85: 4971–4975, 1988.

    Article  PubMed  CAS  Google Scholar 

  14. Stein JC, and Ellsworth ML. Microvascular oxygen transport: impact of a left-shifted dissociation curve. Am J Physiol 262: H5I7–522, 1992.

    Google Scholar 

  15. Lakowicz JR. Quenching of fluorescence. In:Principles of Fluorescence SpectroscopyKluwer Academic,Plenum Publishers, New York, p. 237–65, 1999.

    Google Scholar 

  16. Vanderkooi JM, Maniara G, Green TJ, and Wilson DF. An optical method for measurement of dioxygen concentration based upon quenching of phosphorescence. J Biol Chem 262: 5476–5482, 1987.

    PubMed  CAS  Google Scholar 

  17. Lo LW, Jenkins WT, Vinogradov SA, Evans SM, and Wilson DF. Oxygen distribution in the vasculature of mouse tissue in vivo measured using a near-infrared phosphorAdv Exp Med Biol411: 577–83, 1997.

    Article  PubMed  CAS  Google Scholar 

  18. Buise M., van Bommel J, and Ince C. Reflection spectrophotometryYearbook of Intensive Care and Emergency Medicine 2003(in press)

    Google Scholar 

  19. Benaron DA. Measuring and imaging in tissue using near-IR light.Optics & Photonics News:27–37, 1992.

    Google Scholar 

  20. Steinberg F, Rohrborn HJ, Otto T, Scheufler KM, and Streffer C. NIR reflection measurements of hemoglobin and cytochrome aa3 in healthy tissue and tumors. Correlations to oxygen consumption: Preclinical and clinical data. Adv Exp Med Biol 428: 69–77, 1997.

    CAS  Google Scholar 

  21. Masters BR. Functional imaging of cells and tissues: NADP(H) and flavoprotein redox imaging. In:Medical Optical Tomography: Functional Imaging and Monitoring.Edited by Müller et al. Washington, SPIE-The International Society for Optical Engineering, p. 555–575, 1993.

    Google Scholar 

  22. Chen Z, Milner TE, Wang X, Srinivas S, and Nelson JS. Optical Doppler tomography: imaging in vivo blood flow dynamics following pharmacological intervention and photodynamic therapy. Photochem Photobiol 67: 56–60, 1998.

    Article  PubMed  CAS  Google Scholar 

  23. Li Q, Timmers AM, Hunter K, Gonzalez-Pola C, Lewin AS, Reitze DH, and Hauswirth WW. Noninvasive imaging by optical coherence tomography to monitor retinal degeneration in the mouse. IOVS 42: 2981–2989, 2001.

    CAS  Google Scholar 

  24. Alamouti B, and Funk J. Retinal thickness decreases with age: an OCT study. Br J Ophtalmol 87: 899–901, 2003.

    Article  CAS  Google Scholar 

  25. Bremer C, Bredow S, Mahmood U, Weissleder R, and Tung C-H. Optical imaging of matrix metalloproteinase-2 activity in tumors: feasibility study in a mouse model. Radiology 221: 523–529, 2001.

    Article  PubMed  CAS  Google Scholar 

  26. Dumont EAWJ, Hofstra L, van Heerde WL, van den Eijnde S, Doevendans PAF, DeMuinck E, Daemen MARC, Smits JFM, Frederik P, Wellens HJJ, Daemen MJAP, and Reutelingsperger CPM. Cardiomyocyte death induced by myocardial ischemia and reperfusion: measurement with recombinant human annexin-V in a mouse model. Circulation 102: 1564–1568, 2000.

    Article  PubMed  CAS  Google Scholar 

  27. Mahmood U, Tung C-H, Tang Y, and Weissleder R. Feasibility of in vivo multichannel optical imaging of gene expression: experimental study in mice. Radiology 224: 446–451, 2002.

    Article  PubMed  Google Scholar 

  28. Huang WY, Aramburu J, Douglas PS, and Izumo S. Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy. Nat Med 6: 482–483, 2000.

    Article  PubMed  CAS  Google Scholar 

  29. Vanderkooi JM, and Berger JW. Excited triplet states used to study biological macromolecules at room temperature. Biochim Biophys Acta 976: 1–27, 1989.

    Article  PubMed  CAS  Google Scholar 

  30. Sinaasappel M, and Ince C. Calibration of Pd-porphyrin phosphorescence for oxygen concentration measurements in vivo.JAppl Physiol81: 2297–2303, 1996.

    CAS  Google Scholar 

  31. Vinogradov SA, Fernandez-Seara MA, Dupan BW, and Wilson DF. A method for measuring oxygen distributions in tissue using frequency domain phosphorometry. Comp Biochem Physiol A Mol Integr Physiol 132: 147–152, 2002.

    Article  PubMed  Google Scholar 

  32. Sinaasappel M, van Iterson M, and Ince C. Microvascular oxygen pressure in the pig intestine during hemorrhagic shock and resuscitation.J Physiol514: 245–253, 1999.

    Article  PubMed  CAS  Google Scholar 

  33. Van Iterson M, Sinaasappel M, Burhop K, Trouwborst A, and Ince C. Low-volume resuscitation with a hemoglobin-based oxygen carrier after hemorrhage improves gut microvascular oxygenation in swine.JLab Clin Med132: 421–431, 1998.

    Google Scholar 

  34. Van Iterson M, Siegemund M, Burhop K, and Ince C. Heart and gut microvascular oxygenation in pigs after resuscitation from hemorrhage by different doses of a hemoglobin based oxygen carrier. J Trauma (in press), 2003.

    Google Scholar 

  35. Sato N, Takenobu K, Motoaki S, Kawano S, Abe H, and Hagihara B. Measurement of hemoperfusion and oxygen sufficiency in gastric mucosa in vivo.Gastroeneterology76: 814–819, 1979.

    CAS  Google Scholar 

  36. Dümmler W. Bestimmung von Hämoglobin-Oxygenierung und relativer Hämoglobin-Konzentration in biologische systemen durch auswertung von remissionspektren met hilfe der Kubelka-Munk-theorie. Friedrich-Alexander -Universität, Erlangen-Nürnberg, 1988.

    Google Scholar 

  37. Kubelka P, and Munk F. Ein beitrag zur optik der farbanstriche.Z.Technische Physik 11a: 76–77, 1931.

    Google Scholar 

  38. Kessler M, Frank KH, Höper J, Tauschek D, and Zündorf J. Reflection Spectrometry. In:Oxygen Transport to Tissue XIV.Edited by Erdmann W. and Bruley D.F. New York, Plenum Press, p. 203–212, 1992.

    Book  Google Scholar 

  39. Frank KH, Kessler M, Appelbaum K, and Dummler W. The Erlangen micro-lightguide spectrophotometer EMPHO 1.Phys Med Biol34(12): 1883–1900, 1989.

    Article  PubMed  CAS  Google Scholar 

  40. Kakihana Y, Kessler M, Douplik AJ, and Krug A. Stable and reliable measurements of human skin oxygenation by EMPHO II.SPIE proc optical tomography and spectroscopy of tissue2979, 1997.

    Google Scholar 

  41. Krug A, and Kessler M. Validation and improvements of an algorithm for determination of hemoglobin oxygenations, based on spectral data recorded by tissue spectrophotometer.SPIE2979: 344–354, 1997.

    Article  CAS  Google Scholar 

  42. Siegemund M, van Bommel J, and Ince C. Assessment of regional tissue oxygenation.Intensive Care Med25: 1044–1060, 1999.

    Article  PubMed  CAS  Google Scholar 

  43. Chance B, and Thorell B. Localization and kinetics of reduced pyridine nucleotide in living cells by microfluorometry. J Biol Chem 234: 3044–3050, 1959.

    PubMed  CAS  Google Scholar 

  44. Chance B, and Lieberman M. Intrinsic fluorescence emission from the cornea at low temperatures: evidence of mitochondrial signals and their differing redox states in epithelial and endothelial sides. Exp Eye Res 26: 111–117, 1978.

    Article  PubMed  CAS  Google Scholar 

  45. Ince C, Ashruf JF, Avontuur JAM, Wieringa PA, Spaan JAE, and Bruining HA. Heterogeneity of the hypoxic state in the rat heart is determined at the capillary level.Am JPhysiol264: H294–H301, 1993.

    CAS  Google Scholar 

  46. Duebener LF, Hagino I, Schmitt K, Sakamoto T, Stamm C, Zurakowski D, Schäfers H-J, and Jonas RA. Direct visualization of minimal cerebral capillary flow during retrograde cerebral perfusion: an intravital fluorescence microscopy study in pigs. Ann Thorac Surg 75: 1288–1293, 2003.

    Article  PubMed  Google Scholar 

  47. Coremans JM, Ince C, Bruining HA, and Puppels GJ. (Semi-)quantitative analysis of reduced nicotinamide adenine dinucleotide fluorescence images of blood-perfused rat heart. Biophys J 72: 1849–1860, 1997.

    Article  PubMed  CAS  Google Scholar 

  48. Eng J, Lynch RM, Balaban RS. Nicotinamide adenine dinucleotide fluorescence spectroscopy and imaging of isolated cardiac myocytes. Biophys J 55: 621–630, 1989.

    Article  PubMed  CAS  Google Scholar 

  49. Slaaf DW, Tangelder GJ, Reneman RS, Jäger K, and Bollinger A. A versatile incident illuminator for intravital microscopy.Int JMicrocirc Clin Exp6: 391–397, 1987.

    CAS  Google Scholar 

  50. Hecht E. Optics. Second Edition. Addison-Wesley Publishing Company, Massachusetts, 1987.

    Google Scholar 

  51. Jacques SL, Roman JR, and Lee K. Imaging superficial tissues with polarized light.Lasers Surg Med26: 119–129, 2000.

    Article  PubMed  CAS  Google Scholar 

  52. Groner W, Winkelman WJ, Harris AG, Ince C, Bouma GJ, Messmer K, and Nadeau RG. Orthogonal polarization spectral imaging: a new method for study of the microcirculation.Nat Med5: 1209–1213, 1999.

    Article  PubMed  CAS  Google Scholar 

  53. Mathura KR, Vollebregt KC, Boer K, De Graaff JC, Ubbink DT, and Ince C. Comparison of OPS imaging and conventional capillary microscopy to study the human microcirculation. J Appl Physiol 91: 74–78, 2001.

    PubMed  CAS  Google Scholar 

  54. Mathura KR, Bouma GJ, and Ince C. Abnormal microcirculation in brain tumours during surgery. Lancet 358: 1698–1699, 2001.

    Article  PubMed  CAS  Google Scholar 

  55. Spronk PE, Ince C, Gardien MJ, Mathura KR, Oudemans-van Straaten HM, and Zandstra DF. Nitroglycerin in septic shock after intravascular volume resuscitation. Lancet 360: 1395–1396, 2002.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Academic Medical Center, University of Amsterdam, the Netherlands

    Egbert G. Mik & Can Ince

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  1. Egbert G. Mik
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  2. Can Ince
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  1. Department of Physiology Academic Medical Center, University of Amsterdam, The Netherlands

    Can Ince

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Mik, E.G., Ince, C. (2004). Optical Spectroscopy in Mice. In: Ince, C. (eds) The Physiological Genomics of the Critically Ill Mouse. Basic Science for the Cardiologist, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0483-2_6

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  • DOI: https://doi.org/10.1007/978-1-4615-0483-2_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5099-6

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