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Expression of Sodium-Calcium Exchanger Genes in Heart and Skeletal Muscle Development. Evidence for a Role of Adjacent Cells in Regulation of Transcription and Splicing

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Signal Transduction and Cardiac Hypertrophy

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 7))

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Summary

Sodium-calcium exchangers (NCX) are universal plasmalemma proteins regulating intracellular calcium concentration in animal tissues. While gene structure and regulation of the cardiac-specific isoform NCX1 are known in considerable detail, no data are available on the effect of extrinsic and/or extrinsic factors that govern tissue-specific transcription of all three isoforms (NCX1-NCX3) as well as their still hypothetical alternative splicing. We studied NCX gene expression in cardiac and skeletal muscles and in brain during rat development, as well as their expression in muscle cells grown in presence of neuron-conditioned medium. Our data demonstrate that NCX1 and NCX3 expression in cardiac and skeletal muscle is coordinately regulated and is sensitive to as yet unidentified factors apparently produced by the neighboring cells. Correct splicing of NCX3 transcript is rapidly disrupted within minutes of tissue dissection, consequently most of the previously reported splicing variants featuring exon skipping appear to be artefactual.

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Authors and Affiliations

  1. Faculté des Sciences et des Techniques, Université de Nantes, CNRS UMR, 2, rue de la Houssinière, 6018, Nantes cedex 3, France

    Marie Millour & Dmitri O. Levitsky

  2. INSERM U-437, ITERT, CHU Hôtel Dieu et Faculté des Sciences et des Techniques, Université de Nantes, France

    Laurent Lescaudron

  3. Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada

    Alexander Kraev

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  1. Marie Millour
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  2. Laurent Lescaudron
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  3. Alexander Kraev
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  4. Dmitri O. Levitsky
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Correspondence to Dmitri O. Levitsky .

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Editors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla PhD, MD (Hon), DSc (Hon) (Distinguished Professor and Director), Larry V. Hryshko PhD (Associate Professor), Elissavet Kardami PhD (Professor) & Pawan K. Singal PhD, DSc (Professor) (Distinguished Professor and Director),  (Associate Professor),  (Professor) &  (Professor)

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Millour, M., Lescaudron, L., Kraev, A., Levitsky, D.O. (2003). Expression of Sodium-Calcium Exchanger Genes in Heart and Skeletal Muscle Development. Evidence for a Role of Adjacent Cells in Regulation of Transcription and Splicing. In: Dhalla, N.S., Hryshko, L.V., Kardami, E., Singal, P.K. (eds) Signal Transduction and Cardiac Hypertrophy. Progress in Experimental Cardiology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0347-7_9

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  • DOI: https://doi.org/10.1007/978-1-4615-0347-7_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5032-3

  • Online ISBN: 978-1-4615-0347-7

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