Raman Spectroscopy for Whole Organism and Tissue Profiling

  • Sarah Clarke
  • Royston Goodacre


The inelastic light scattering process that has become eponymously known as Raman scattering was first observed in 1928 by the Indian physicist Chandrasekhara Venkata Raman and reported in Nature (Raman and Krishnan, 1928). Raman scattering spectra, like infrared (IR) absorption spectra, originate from an exchange of energy between photons and vibrational or rotational motions in molecules (Nelson, 1985). Raman scattering is a light scattering phenomenon in which an incident photon beam of well-defined wavelength (a monochromatic laser) is scattered by molecules. While most of the radiation is scattered elastically (Rayleigh scattering), a small fraction of the photons are modified as an irradiated molecule undergoes a vibrational transition (Adar et al., 1997).


Raman Spectroscopy Ultra Violet Surface Enhance Raman Spectroscopy Resonance Raman Spectroscopy Resonance Raman Scattering 
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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Sarah Clarke
    • 1
  • Royston Goodacre
    • 1
    • 2
  1. 1.Institute of Biologial Scicences University of WalesAbersytwythUK
  2. 2.Department of ChemistryUniversity of Manchester Institute of ScienceManchesterUK

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