Raman Spectroscopy for Whole Organism and Tissue Profiling

  • Sarah Clarke
  • Royston Goodacre

Abstract

The inelastic light scattering process that has become eponymously known as Raman scattering was first observed in 1928 by the Indian physicist Chandrasekhara Venkata Raman and reported in Nature (Raman and Krishnan, 1928). Raman scattering spectra, like infrared (IR) absorption spectra, originate from an exchange of energy between photons and vibrational or rotational motions in molecules (Nelson, 1985). Raman scattering is a light scattering phenomenon in which an incident photon beam of well-defined wavelength (a monochromatic laser) is scattered by molecules. While most of the radiation is scattered elastically (Rayleigh scattering), a small fraction of the photons are modified as an irradiated molecule undergoes a vibrational transition (Adar et al., 1997).

Keywords

Biomass Cholesterol Fermentation Urea Lactate 

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References

  1. Adar F, Geiger R, Noonan J. Raman spectroscopy for process/ quality control. Appl Spectr 32: 45–101 (1997).CrossRefGoogle Scholar
  2. Angel MA, Myrick ML. Wavelength selection for fiber optic Raman spectroscopy. Appl Optics 29: 1350–1352 (1990).CrossRefGoogle Scholar
  3. Asher SA. UV resonance Raman spectroscopy for analytical, physical and biophysical chemistry — Part 1. Anal Chem 65: 59A–66A (1993a).Google Scholar
  4. Asher SA. UV resonance Raman spectroscopy for analytical, physical and biophysical chemistry — Part 2. Anal Chem 65: 201A–210A (1993b).PubMedGoogle Scholar
  5. Asher SA, Munro CH, Chi ZH. UV lasers revolutionize Raman spectroscopy. Laser Focus World 33: 99–109 (1997).Google Scholar
  6. Barthus RC, Poppi RJ. Determination of the total unsaturation in vegetable oils by Fourier transform Raman spectroscopy and multivariate calibration. Vibrat Spectr 26: 99–105 (2001).CrossRefGoogle Scholar
  7. Berger AJ, Itzkan I, Feld MS. Feasibility of measuring blood glucose concentration by near-infrared Raman spectroscopy. Spectrochim Acta 53: 287–292 (1997).Google Scholar
  8. Berger AJ, Koo T-W, Itzkan I et al. Multicomponent blood analysis by near-infrared Raman spectroscopy. Appl Optics 38: 2916–2926 (1999).CrossRefGoogle Scholar
  9. Berger AJ, Wang Y, Feld MS. Rapid, noninvasive concentration measurements of aqueous biological analytes by near-infrared Raman spectroscopy. Appl Optics 35: 209–212 (1996).CrossRefGoogle Scholar
  10. Boustany NN, Crawford JM, Manoharan R et al. Analysis of nucleotides and aromatic amino acids in normal and neoplastic colon mucosa by ultraviolet resonance Raman spectroscopy. Lab Investirai 79: 1201–1214 (1999).Google Scholar
  11. Brown R, Smith WE, Graham D. Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces. Tet Lett 42: 2197–2200 (2001).CrossRefGoogle Scholar
  12. Chase B. A new generation of Raman instrumentation. Appl Spectr 48: 14A–19A (1994).CrossRefGoogle Scholar
  13. Chen XG, Lemmon DH, Bormett RW, Asher SA. Convenient microsampling system for UV resonance Raman spectroscopy. Appl Spectr 47: 248–249 (1993).CrossRefGoogle Scholar
  14. Chi ZH, Asher SA. Ultraviolet resonance Raman examination of horse apomyoglobin acid unfolding intermediates. Biochemistry 38: 8196–8203 (1999).PubMedCrossRefGoogle Scholar
  15. Choo-Smith LP, Maquelin K, van Vreeswijk T et al. Investigating microbial (micro)colony heterogeneity by vibrational spectroscopy. Appl Environ Microbiol 67: 1461–1469 (2001).PubMedCrossRefGoogle Scholar
  16. Colthup NB, Daly LH, Wiberly SE. Introduction to Infrared and Raman Spectroscopy. Academic Press, New York (1990).Google Scholar
  17. Dereniak EL, Crowe DGiR. Optical Radiation Detectors. John Wiley and Sons, New York (1984).Google Scholar
  18. Dou X, Yamaguchi Y, Yamamoto H et al. Quantitative analysis of metabolites in urine using a highly precise, compact near-infrared Raman spectrometer. Vibrat Spectr 13: 83–89 (1996).CrossRefGoogle Scholar
  19. Dou X, Yamaguchi Y, Yamamoto H et al. Quantitative analysis of metabolites in urine by anti-Stokes Raman spectroscopy. Biospectroscopy 3: 113–120 (1997).CrossRefGoogle Scholar
  20. Edwards HGM, Farwell DW, Seaward MRD, Giacobini C. Preliminary Raman microscopic analyses of a lichen encrustation involved in the biodeterioration of Renaissance frescoes in central Italy. Internat Biodeteriorat Biodegrad 27: 1–9 (1991).Google Scholar
  21. Edwards HGM, Holder JM, Wynn-Williams DD. Comparative FT-Raman spectroscopy of Xanthoria lichen-substratum systems from temperate and Antartic habitats. Soil Biol Biochem 30: 1947–1953 (1998).CrossRefGoogle Scholar
  22. Efremov RG, Feofanov AV, Nabiev IR. Quantitative treatment of UV resonance Raman spectra of biological molecules — application to the study of membrane-bound proteins. Appl Spectr 45: 272–278 (1991).CrossRefGoogle Scholar
  23. Ferraro JR, Nakamoto K. Introductory Raman Spectroscopy. Academic Press, London (1994).Google Scholar
  24. Gfrorer A, Schnetter ME, Wolfrum J, Greulich KO. Double and triple helices of nucleic acid polymers, studied by UV resonance Raman spectroscopy. Berichte der Bunsen-Gesellschaft-Phys Chem Chem Phys 97: 155–162 (1993).CrossRefGoogle Scholar
  25. Ghiamati E, Manoharan R, Nelson WH, Sperry JF. UV resonance Raman spectra of Bacillus spores. Appl Spectr 46: 357–364 (1992).CrossRefGoogle Scholar
  26. Goodacre R, Timmins ÉM, Burton R et al. Rapid identification of urinary tract infection bacteria using hyperspectral, whole organism fingerprinting and artificial neural networks. Microbiol 144: 1157–1170 (1998).Google Scholar
  27. Hanlon EB, Manoharan R, Koo T-W et al. Prospects for in vivo Raman spectroscopy. Phys Med Biol 45: Rl–R59 (2000).CrossRefGoogle Scholar
  28. Heise HM, Bittner A, Marbach R. Near-infrared reflectance spectroscopy for noninvasive monitoring of metabolites. Clin Chem Lab Med 38: 137–145 (2000).PubMedCrossRefGoogle Scholar
  29. Holtz JSW, Holtz JH, Chi ZH, Asher SA. Ultraviolet Raman examination of the environmental dependence of bombolitin I and bombolitin III secondary structure. Biophys.776: 3227–3234 (1999).CrossRefGoogle Scholar
  30. Kaminaka S, Imamura Y, Shingu H et al. Studies of bovine enterovirus structure by ultraviolet resonance Raman spectroscopy. J Virol Meth 77: 117–123 (1999).CrossRefGoogle Scholar
  31. Kirschner C, Maquelin K, Pina P et al. Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. J Clin Microbiol 39: 1763–1770 (2001).PubMedCrossRefGoogle Scholar
  32. Kitagawa T. Investigation of higher order structures of proteins by ultraviolet resonance Raman spectroscopy. Prog Biophys Mol Biol 58: 1–18 (1992).PubMedCrossRefGoogle Scholar
  33. Kneipp K, Kneipp H, Itzkan I et al. Surface-enhanced Raman scattering: a new tool for biomedical spectroscopy. Curr Sci 77: 915–924 (1999).Google Scholar
  34. Lecomte S, Moreau NJ, Manfait M et al. Surface-enhanced Raman spectroscopy investigation of fluoroquinoline/ DNA/ DNA gyrase/ Mg2+ interactions: Part 1. adsorption of Pefloxacin on colloidal silver-effect of drug concentration, electrolytes, and pH. Biospectroscopy 1: 423–436 (1995).CrossRefGoogle Scholar
  35. Lednev IK, Karnoup AS, Sparrow MC, Asher SA. Alpha-helix peptide folding and unfolding activation barriers: a nanosecond UV resonance Raman study. J Am Chem Soc 121: 8074–8086 (1999).CrossRefGoogle Scholar
  36. Manoharan R, Ghiamati E, Chadha S et al. Effect of cultural conditions on deep UV resonance Raman spectra of bacteria. Appl Spectr 47: 2145–2150 (1993).CrossRefGoogle Scholar
  37. Manoharan R, Ghiamati E, Dalterio RA et al. UV resonance Raman spectra of bacteria, bacterial spores, protoplasts and calcium dipicolinate. J Microbiol Meth 11: 1–15 (1990).CrossRefGoogle Scholar
  38. Manoharan R, Wang Y, Feld MS. Histochemical analysis of biological tissues using Raman spectroscopy. Spectrochim Acta Part A: 215–249 (1996).Google Scholar
  39. Maquelin K. Confocal Raman Microspectroscopy. A Novel Diagnostic Tool in Medical Microbiology, Erasmus University, Rotterdam (2002).Google Scholar
  40. Maquelin K, Choo-Smith L-P, van Vreeswijk T et al. Raman spectroscopic method for identification of clinically relevant microorganisms growing on solid culture medium. Anal Chem 72: 12–19 (2000).PubMedCrossRefGoogle Scholar
  41. McAnally G, McLaughlin C, Brown R et al. SERRS dyes. Part I. Synthesis of benzotriazole monoazo dyes as model analytes for surface enhanced resonance Raman scattering. Analyst 127: 838–841 (2002).PubMedCrossRefGoogle Scholar
  42. McCreery RL. CCD array detectors for multichannel Raman spectroscopy. In Charge Transfer Devices in Spectroscopy. Sweedler J, Ratzlaff K, Denton M (Ed) pp. 227–229, VCH, New York (1994).Google Scholar
  43. McCreery RL. Instrumentation for dispersive Raman Sspectroscopy. In Modern Techniques in Raman Spectroscopy. Vol. 1. Laserna JJ (Ed) pp. 41–72, John Wiley and Sons, Chichester (1996).Google Scholar
  44. McGovern AC, Broadhurst D, Taylor J et al. Monitoring of complex industrial bioprocesses for metabolite concentrations using modern spectroscopies and machine learning: application to gibberellic acid production. Biotechnol Bioeng 78: 527–538 (2002).PubMedCrossRefGoogle Scholar
  45. Moskovits M. Surface-enhanced Raman spectroscopy. Rev Mod Phys 57: 783 (1985).CrossRefGoogle Scholar
  46. Mukerji I, Shiber MC, Fresco JR, Spiro TG. A UV resonance Raman study of hairpin dimer helices of d(A-G)(10) at neutral pH containing intercalated dA residues and alternating dG tetrads. Nucleic Acids Res 24: 5013–5020 (1996).PubMedCrossRefGoogle Scholar
  47. Munro CH, Asher SA. UV lasers light the way for novel spectroscopy. Photonics Spectra 30: 118–120 (1996).Google Scholar
  48. Nabiev I, Manfait M. Industrial applications of the surface-enhanced Raman-spectroscopy. Rev Industr Fr Petrol 48: 261–285 (1993).Google Scholar
  49. Nelson WH. Instrumental Methods for Rapid Microbiological Analysis. VCH Publishers (1985).Google Scholar
  50. Nelson WH, Manoharan R, Sperry JF. UV resonance Raman studies of bacteria. Appl Spectr Rev 27: 67–124 (1992a).CrossRefGoogle Scholar
  51. Nelson WH, Manoharan R, Sperry JF. UV resonance Raman studies of bacteria. Appl Spectr Rev 27: 67–124 (1992b).CrossRefGoogle Scholar
  52. Nelson WH, Sperry JF. UV resonance Raman spectroscopic detection and identification of bacteria and other microorganisms. In Modern Techniques for Rapid Microbiological Analysis. Nelson WH (Ed) pp. 97–143, VCH Publishers, New York (1991).Google Scholar
  53. Overman SA, Thomas GJ. Novel vibrational assignments for proteins from Raman spectra of viruses. J Raman Spectr 29: 23–29 (1998).CrossRefGoogle Scholar
  54. Pal A, Stokes DL, Alarie JP, Vo-Dinh T. Selective surface-enhanced Raman spectroscopy using a polymer coated-substrate. Anal Chem 67: 3154–3159 (1995).CrossRefGoogle Scholar
  55. Pilotto S, Pacheco MTT, Silveira L et al. Analysis of near-infrared Raman spectroscopy as a new technique for a transcutaneous non-invasive diagnosis of blood components. Lasers Med Sci 6: 2–9 (2000).Google Scholar
  56. Prescott B, Steinmetz W, Thomas GJ. Characterisation of DNA structures by laser Raman spectroscopy. Biopolymers 23: 235–256 (1984).PubMedCrossRefGoogle Scholar
  57. Qu JY, Wilson BC, Suria D. Concentration measurements of multiple analytes in human sera by near-infrared laser Raman spectroscopy. Appl Optics 38: 5491–5498 (1999).CrossRefGoogle Scholar
  58. Raman CV, Krishnan KS. A new type of secondary radiation. Nature 121: 501 (1928).CrossRefGoogle Scholar
  59. Roberts MJ, Garrison AA, Kercel SW, Muly EC. Raman spectrocopy for on-line, real-time, multi-point industrial chemical analysis. Process Control Quality 1: 281–291 (1991).Google Scholar
  60. Sanford CL, Mantooth BA, Jones BT. Determination of ethanol in alcohol samples using a modular Raman spectrometer. J Chem Ed 78: 1221–1224 (2001).CrossRefGoogle Scholar
  61. Sato H, Chiba H, Tashiro H, Ozaki Y. Excitation wavelength-dependent changes in Raman spectra of whole blood and hemoglobin: comparison of the spectra with 514.5-, 720-, and 1064-nm excitation. J Biomed Optics 6: 366–370 (2001).CrossRefGoogle Scholar
  62. Schuster KC, Reese I, Urlaub E et al. Multidimensional information on the chemical composition of single bacterial cells by confocal Raman microspectroscopy. Anal Chem 72: 5529–5534 (2000).PubMedCrossRefGoogle Scholar
  63. Shaw AD, Kaderbhai N, Jones A et al. Noninvasive, on-line monitoring of the biotransformation by yeast of glucose to ethanol using dispersive Raman spectroscopy and chemometrics. Appl Spectr 53: 1419–1428 (1999).CrossRefGoogle Scholar
  64. Stevenson CL, Vo-Dinh T. Signal expressions in Raman spectroscopy. In Modern Techniques in Raman Spectroscopy. Vol. 1. Laserna JJ (Ed) pp. 1–39, John Wiley and Sons, Chichester (1996).Google Scholar
  65. Ullas G, Sudhaker SN, Gopalakrishna K et al. Laser Raman spectroscopy: some clinical applications. Curr Sci 77 908–914 (1999).Google Scholar
  66. Urlaub E, Popp J, Keifer W et al. FT-Raman investigation of alkaloids in the liana Ancistrocladus heyneanus. Biospectroscopy 4: 113–120 (1998).PubMedCrossRefGoogle Scholar
  67. Venkatakrishna K, Kurien J, Pai KM et al. Optical pathology of oral tissue: a Raman spectroscopy diagnostic method. Curr Sci 80: 665–669 (2000).Google Scholar
  68. Wang SY, Hasty CE, Watson PA et al. Analysis of metabolites in aqueous solutions by using laser Raman spectroscopy. Appl Optics 32: 925–929 (1993).CrossRefGoogle Scholar
  69. Wen ZQ, Thomas GJ. UV resonance Raman spectroscopy of DNA and protein constituents of viruses: Assignments and cross sections for excitations at 257, 244, 238, and 229 nm. Biopolymers 45: 247–256 (1998).PubMedCrossRefGoogle Scholar
  70. Williams KPJ, Pitt GD, Batchelder DN, Kip BJ. Confocal Raman microspectroscopy using a stigmatic spectrograph and CCD detector. Appl Spectr 48: 232–235 (1994).CrossRefGoogle Scholar
  71. Williams KPJP, Pitt GD. Smith,BJE, Whitley A. Use of a rapid scanning stigmatic Raman imaging spectrograph in the industrial environment. J Raman Spectr 25: 131–138 (1994).CrossRefGoogle Scholar
  72. Wynn-Williams DD, Edwards HGM, Russell NC. Moisture and habitat structure as regulators for microalgal colonists in diverse Antartic terrestrial habitats. In Ecosystem Processes in Antartic Ice-Free Landscapes. Howard-Williams C, Lyons B, Hawes I (Ed) pp. 77–78, Balkema Press, Rotterdam (1997).Google Scholar
  73. Yazdi Y, Ramanujam N, Lotan R et al. Resonance Raman spectroscopy at 257 nm excitation of normal and malignant cultured breast and cervical cells. Appl Spectr 53: 82–85 (1999).CrossRefGoogle Scholar
  74. Zhelyaskov VR, Milne ET, Hetke JF, Morris MD. Silicon substrate microelectrode array for surface-enhanced Raman spectroscopy. Appl Spectr 49: 1793–1795 (1995).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Sarah Clarke
    • 1
  • Royston Goodacre
    • 1
    • 2
  1. 1.Institute of Biologial Scicences University of WalesAbersytwythUK
  2. 2.Department of ChemistryUniversity of Manchester Institute of ScienceManchesterUK

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