Bcl10 Protein can Act as a transcription activator in yeast

  • Ming Chen
  • Ling Yun Li
  • Yi-Peng Qi
Part of the Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease book series (DMCB, volume 41)

Abstract

The Bcl10 gene has recently been cloned from the chromosomal translocation t(1:14) (p22; q32) in a low-grade mucosa-associated lymphoid tissue (MALT) lymphoma, and was implicated in the pathogenesis of this and several other tumor types. In yeast two hybrid systems, when it was fused to Ga14 DNA-binding domain of pGBT9, this fusion protein can activate the expression of reporter genes without Ga14-AD domain. Through deletion assay and secondary structure prediction, we found that the N-terminal of Bcl10 contributed more to activation than the C-terminal. It has been recently reported that Bcl10 expression is correlated with the activation of NF-KB in eukaryotic cells, so it may represent an important role in protein expression. Our research is the first to demonstrate a new function of Bcl10: transcription activation in yeast. (Mol Cell Biochem 246: 97–103,2003)

Keywords

Bcl10 transcription activator yeast hybrid system 

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Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  • Ming Chen
    • 1
  • Ling Yun Li
    • 1
  • Yi-Peng Qi
    • 1
  1. 1.Wu Han University, School of Life SciencesMolecular Virology LaboratoryWu HanP.R. China

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