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L-Canavanine as a radiosensitization agent for human pancreatic cancer cells

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Guanidino Compounds in Biology and Medicine

Part of the book series: Molecular and Cellular Biochemistry ((DMCB,volume 40))

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Abstract

This study evaluated the in vitro effect of L-canavanine on cell cycle progression in the two human pancreatic cancer cells lines PANC-1 and MIA PaCa-2. After 72 h of exposure to L-canavanine, the percentage of cells in the radiosensitive G2/M phase of the cell cycle increased 6-fold in PANC-1 cells and 4-fold in MIA PaCa-2 cells, when compared to untreated cells. The capacity of L-canavanine to redistribute cells into the G2/M phase of the cell cycle was both concentration-and time-dependent. Since many drugs that cause cells to accumulate in the G2/M phase of the cell cycle are effective radiosensitization agents, the potential of L-canavanine to synergistically enhance the effects of ionizing radiation also was evaluated. The interaction between these treatment modalities was quantified using the median-effect equation and combination index analysis. LCanavanine was found to be synergistic with radiation when either PANC-1 or MIA PaCa-2 cells were exposed to L-canavanine for 72 h prior to irradiation. These results suggest that L-canavanine in combination with radiation may have clinical potential in the treatment of pancreatic cancer. (Mol Cell Biochem 244: 37-43, 2003)

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Authors and Affiliations

  1. Division of Pharmaceutical Sciences, USA

    Aimee K. Bence & Peter A. Crooks

  2. Division of Pharmacy Practice and Science, College of Pharmacy, University of Kentucky, Lexington, KY, USA

    Val R. Adams

Authors
  1. Aimee K. Bence
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  2. Val R. Adams
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  3. Peter A. Crooks
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Editor information

Editors and Affiliations

  1. Department of Neurology, University of Cincinnati Medical Center, Cincinnati, OH, 45267-0536, USA

    Joseph F. Clark

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© 2003 Springer Science+Business Media New York

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Bence, A.K., Adams, V.R., Crooks, P.A. (2003). L-Canavanine as a radiosensitization agent for human pancreatic cancer cells. In: Clark, J.F. (eds) Guanidino Compounds in Biology and Medicine. Molecular and Cellular Biochemistry, vol 40. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0247-0_5

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  • DOI: https://doi.org/10.1007/978-1-4615-0247-0_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4985-3

  • Online ISBN: 978-1-4615-0247-0

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