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Enzyme Immunoassays for 15-F2t Isoprostane-M, an Urinary Biomarker for Oxidant Stress

  • Diane M. Sasaki
  • Ying Yuan
  • Katherine Gikas
  • Kazuo Kanai
  • Douglass Taber
  • D. Jason Morrow
  • L. Jackson RobertsII
  • Denis M. Callewaert
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)

Abstract

Isoprostane assays have significant potential for use as molecular biomarkers of human oxidant stress (Morrow et al. 1999, Lawson et al. 1999). Recent studies indicate that: (a) significant amounts of F2 isoprostanes derived from systemic oxidant stress are metabolized before excretion, (b) a significant quantity of free F2 isoprostanes are produced by the kidney, so free isoprostanes detected in urine may not accurately reflect systemic oxidant stress and (c) 2,3-dinor-5,6-dihydro-15-F2tIsoprostane (F2-IsoP-M, IsoP-M), a major urinary metabolite of systemic 8-iso-PG F2Œ, has recently been identified (Figure 1). Urinary F2-IsoP-M may, therefore, prove to be an excellent marker for systemic oxidant stress. Our goal is to develop a sensitive and specific immunoassay for F2-IsoP-M, which should provide a simple non-invasive method for routine quantitative assessment of human oxidant stress.

Keywords

High Pressure Liquid Chromatography Caprylic Acid Immunometric Assay Systemic Oxidant Stress Competitive Immunoassay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Diane M. Sasaki
    • 1
  • Ying Yuan
    • 1
  • Katherine Gikas
    • 1
  • Kazuo Kanai
    • 2
  • Douglass Taber
    • 2
  • D. Jason Morrow
    • 3
  • L. Jackson RobertsII
    • 3
  • Denis M. Callewaert
    • 1
    • 4
  1. 1.Oxford Biomedical ResearchOxfordUSA
  2. 2.Dept. of Chemistry and BiochemistryUniversity of DelawareNewarkUSA
  3. 3.Dept. of Medicine and PharmacologyVanderbilt UniversityNashvilleUSA
  4. 4.Dept. of ChemistryOakland UniversityRochesterUSA

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