Morphological and Electrophysiological Studies of Substantia Nigra, Tegmental Pedunculopontine Nucleus, and Subthalamus in Organotypic Co-Culture

  • S. T. Kitai
  • N. Ichinohe
  • J. Rohrbacher
  • B. Teng
Part of the Advances in Behavioral Biology book series (ABBI, volume 54)


Recently we incorporated an organotypic culture method in our basal ganglia research. In our preparation, we were successful in co-culturing more than two structures of interest1–3. This in vitro organotypic preparation combines the advantage of in vitro slice preparation for ease of intracellular sharp or patch recording under improved controlled experimental chemical environment with the in vivo preparation in which the structure of interest is not isolated from the source of their major afferents. In this report, we would like to present a triple culture preparation consisting of the tegmental pedunculopontine nucleus (PPN), the subthalamic nucleus (STN) and the substantia nigra (SN).


Substantia Nigra Cholinergic Neuron Subthalamic Nucleus Organotypic Culture Slice Preparation 
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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • S. T. Kitai
    • 1
  • N. Ichinohe
    • 1
  • J. Rohrbacher
    • 1
  • B. Teng
    • 1
  1. 1.Department of Anatomy and NeurobiologyThe University of Tennessee, College of MedicineTennesseeUSA

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