HPLC Profiling of Trp-related Metabolites In Humans

  • Eliška Marklová
  • Ziad Albahri
  • Marie Nožičková
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 527)


Screening for metabolic abnormalities of Trp has been introduced using SPE pretreatment, TLC and/or two HPLC procedures. The excretory pattern in urine (occasionally also plasma and CSF levels) has been followed in a group of 390 children showing various symptoms of a metabolic defect and in 195 patients with skin diseases, namely those associated with photosensitivity, such as porphyria, vitiligo, alopecia, psoriasis, erythematodes, and others. Excretory abnormalities of either indican, kynurenine, 3-hydroxyanthranilic acid or indolylacryloylglycine have been occasionally combined with myopathy, seizures, liver and intestinal symptoms. Several indoles and kynurenine derivatives present changes in the alopecia group and the vitiliginous patients.


Metabolic Defect Common Variable Immunodeficiency Porphyria Cutanea Tarda HPLC Procedure Sulphosalicylic Acid 
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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Eliška Marklová
    • 1
  • Ziad Albahri
    • 2
  • Marie Nožičková
    • 2
  1. 1.Faculty of Medicine, Department of PaediatricsChaṙles UniversityCzech Republic
  2. 2.Department of DermatologyChaṙles UniversityCzechRepublic

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